Genomic DNA Extraction Kit

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Q&A

What is the key to successfully isolating genomic DNA of good yield and quality?

Plasmid DNA Extraction Kit(PD004, PD100, PD300) --Q&A
　

Q	Why do we sometimes obtain a lower yield?
Q	Why doesn’t the Eluted DNA perform well in downstream applications?
Q	Can the plasmid DNA extraction Kit be applied on Gram (+) bacteria?
Q	Why do we get eluted plasmid contaminated with RNA?
Q	Why can’t the eluted plasmid be cut by restriction enzymes properly?
Q	Why does the Plasmid DNA Extraction Kit require two washings with two types of wash buffers (W1 and Wash Buffer) while competitors kits require one washing?
Q	Eluted plasmid appeared degraded after storage for an extended period of time.
Q	Why was Restriction enzyme digestion unsuccessful?

Genomic DNA Extraction Kit(GT004,GT050,GT300,GB004,GB100,GB300,GP004,GP100)--Q&A
　

Q	What should be done if the Column is clogged?
Q	Why is the DNA recovery lower than expected?
Q	Why doesn’t the Eluted DNA perform well in downstream applications?
Q	Why does the genomic DNA band appear smeared in agarose gel electrophoresis?
Q	What is the key to successfully isolating genomic DNA of good yield and quality?
A	The key is to use fresh samples and not to overload the column. Low yield or purity of genomic DNA is usually due to incomplete digestion or incomplete Lysis of the sample. Starting with a maximum amount or volume of samples does NOT usually give the best yield of DNA. On the contrary, it usually results in incomplete sample Lysis and degradation of proteins, thus making extraction of all DNA from the sample unfeasible. Further, it always requires subsequent removal of undigested residues and yields viscous sample lysate. When the lysate is too viscous, it not only has difficulty in passing the column, but also indicates the presence of an abundant amount of contaminants such as proteins and salts. High amounts of contaminants not only affect DNA binding, but also may not be washed off completely, leading to carry over to the eluted genomic DNA. Therefore, a good quality and yield of DNA is only expected when a sample is completely digested. We advise starting with half of the maximum amount of sample suggested. When there aren’t any problems with digestion or passing the lysate through the column, the sample amount can be increased gradually in the subsequent preparations.
Q	Why does the sample appear viscous after sample Lysis and not pass through the column easily, even when less than the suggested maximum sample amount is used?
Q	When Buffy coat is used, the column tends to be clogged.

Gel/PCR DNA Fragments Extraction Kit (DF004, DF100, DF300)--Q&A

Q	Can the Gel/PCR DNA Kit completely remove primers or dimer products from PCR reactions?
Q	Can the Gel/PCR DNA Fragments Extraction Kit be used to clean up DIG-labeled DNA fragment?
Q	A band, smaller than the desired DNA fragment is present in the eluant.
Q	Why DNA recovery is lower than expected?
Q	Why doesn’t the Eluted DNA perform well in downstream applications?

RNA Related Products(RT050,RT100,RT300,RB050,RB100,RB300,RP050,RP100)--Q&A

Q	How do I increase yields of total RNA?
Q	Why does the obtained RNA appear smeared and degraded?
Q	Is total RNA isolated by the Total RNA Extraction System free of genomic DNA?
Q	What is the most suitable kit for Cytoplasmic RNA extraction?
Q	What is the function of the lysate filter columns?
Q	Which kit is suitable for RNA extraction from yeast?