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Q&A

How do I increase yields of total RNA?

Plasmid DNA Extraction Kit(PD004, PD100, PD300) --Q&A
　

Q	Why do we sometimes obtain a lower yield?
Q	Why doesn’t the Eluted DNA perform well in downstream applications?
Q	Can the plasmid DNA extraction Kit be applied on Gram (+) bacteria?
Q	Why do we get eluted plasmid contaminated with RNA?
Q	Why can’t the eluted plasmid be cut by restriction enzymes properly?
Q	Why does the Plasmid DNA Extraction Kit require two washings with two types of wash buffers (W1 and Wash Buffer) while competitors kits require one washing?
Q	Eluted plasmid appeared degraded after storage for an extended period of time.
Q	Why was Restriction enzyme digestion unsuccessful?

Genomic DNA Extraction Kit(GT004,GT050,GT300,GB004,GB100,GB300,GP004,GP100)--Q&A
　

Q	What should be done if the Column is clogged?
Q	Why is the DNA recovery lower than expected?
Q	Why doesn’t the Eluted DNA perform well in downstream applications?
Q	Why does the genomic DNA band appear smeared in agarose gel electrophoresis?
Q	What is the key to successfully isolating genomic DNA of good yield and quality?
Q	Why does the sample appear viscous after sample Lysis and not pass through the column easily, even when less than the suggested maximum sample amount is used?
Q	When Buffy coat is used, the column tends to be clogged.

Gel/PCR DNA Fragments Extraction Kit (DF004, DF100, DF300)--Q&A

Q	Can the Gel/PCR DNA Kit completely remove primers or dimer products from PCR reactions?
Q	Can the Gel/PCR DNA Fragments Extraction Kit be used to clean up DIG-labeled DNA fragment?
Q	A band, smaller than the desired DNA fragment is present in the eluant.
Q	Why DNA recovery is lower than expected?
Q	Why doesn’t the Eluted DNA perform well in downstream applications?

RNA Related Products(RT050,RT100,RT300,RB050,RB100,RB300,RP050,RP100)--Q&A

Q	How do I increase yields of total RNA?
A	(1) Poor yield of total RNA is mostly due to incomplete sample Lysis, thus leading to incomplete release of RNA. Since good yield and good quality of total RNA are only assured when the sample is properly handled and lysed completely, DO NOT use more than the amount of sample suggested in the protocol. (2) Thorough cellular disruption is critical for high RNA quality and yield. RNA that is trapped in intact cells is often removed with cellular debris and is unavailable for subsequent isolation. Therefore, it is crucial to choose the disruption method best suited to a specific tissue or organism to maximize yield. Mechanical cell disruption techniques include grinding, homogenization, vortexing, sonication etc. Complete disruption of some tissues may require using a combination of these techniques. (3) Another, more common cause of low RNA yield is overloading the column, which can cause the column to clog or can prevent the RNA from binding to the membrane efficiently. Methods that reduce viscosity, such as reducing sample amount, disrupting the sample more extensively, and centrifuging to remove insoluble remains, will increase RNA yield. If yields are still lower than expected, consider diluting the clarified lysate and splitting loading into two columns, which will further reduce the concentration of contaminants and improve RNA binding and recovery. (4) When RNA is to be eluted, make sure that RNase-free ddH2O is added onto the membrane and penetrate into it. If ddH2O still remains on the membrane, pulse centrifuge the column for a few seconds to drag it into the membrane.
Q	Why does the obtained RNA appear smeared and degraded?
Q	Is total RNA isolated by the Total RNA Extraction System free of genomic DNA?
Q	What is the most suitable kit for Cytoplasmic RNA extraction?
Q	What is the function of the lysate filter columns?
Q	Which kit is suitable for RNA extraction from yeast?