Bacteria RNA Mini Kit (RBBD050/RBBD100/RBBD300) with DNase

Bacteria RNA Kit with DNase Set
Bacteria RNA Mini Kit (RBBD050/RBBD100/RBBD300) with DNase
The Presto™ Mini RNA Bacteria Kit was designed for total RNA purification from Gram (-) negative bacteria and Gram (+) positive bacteria using an efficient RNA miniprep system. This RNA Kit includes Bacteria Lysis Buffer and Lysozyme to reduce sample preparation time and minimize hands on time.  The Presto™ Mini RNA Bacteria Kit uses detergents and chaotropic salt to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound by the glass fiber matrix of the RNA spin column and once any contaminants have been removed, using the Wash Buffer, the purified total RNA is eluted by RNase-free Water. High quality total RNA can be purified within 20 minutes and is ready for use in RT-PCR, Northern Blotting, Primer Extension, mRNA Selection and cDNA Synthesis.  Each Geneaid RNA Kit includes individually packaged certified DNase and RNase-free spin columns and collection tubes and DNase I for DNA sensitive downstream applications.

 

 

Advantages (Cat. # RBBD050, RBBD100, RBBD300)

  • Purify total RNA within 20 minutes!
  • High Yield: up to 60 μg of pure RNA (1 x 109 Escherichia coli: 40-45 µg, 1 x 109 Bacillus subtilis: 50-55 µg)
  • Wide Variety of Bacteria Sample Strains: Gram (+) Positive and Gram (-) Negative bacterial cells (up to 1 x 109)
  • Convenient: Includes Bacteria Lysis Buffer and Lysozyme
  • Spin Columns: glass fiber membrane optimized for total RNA extraction
  • Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
  • Storage: dry at room temperature (15-25°C), Lysozyme and DNase I are shipped at room temperature and should be stored at -20°C for extended periods
Applications
 
RT-PCR, Northern Blotting, Primer Extension, mRNA Selection, cDNA Synthesis
 
Components
  • Bacteria Lysis Buffer
  • Lysozyme
  • RB Buffer
  • DNase I
  • DNase Reaction Buffer
  • W1 Buffer
  • Wash Buffer
  • RNase-free Water
  • RB Columns
  • 2 ml Collection Tubes

Quality Control

The quality of the Presto™ Mini RNA  Bacteria Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. The purified RNA from Escherichia coli cells (up to 1 x 109), (A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.

Presto™ Mini RNA Bacteria Kit Functional Test Data



Figure 1. RNA was extracted using the Presto™ Mini RNA Bacteria Kit. An Escherichia coli (1×109) culture (OD600=1.3, 1 ml) was harvested by centrifugation at 16,000 x g for 1 minute. 10 µl from a 50 µl eluate of purified RNA was analyzed by electrophoresis on a 0.8% agarose gel.

Sample Yield 260/280 260/230
1 41.56 µg 2.14 2.35
2 40.87 µg 2.15 2.32

Total RNA Extraction Kits Specifications

Specifications
Cat. #
RBD050
RBD100
RBD300
RTD050
RTD100
RTD300
RPD050
RPD100
RPD300
RBBD050
RBBD100
RBBD300
RBYD050
RBYD100
RBYD300
RMI050
GZXD050
GZXD100
GZXD200
Format
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Tri Reagent Spin Column
Binding Capacity 50 µg 50 µg 50 µg 50 µg 50 µg 50 µg 50 µg
Sample
blood, cells
animal tissue plant tissue bacterial cells  yeast cells animal tissue, cells
blood,cells,
tissue, body fluids, bacteria
Micropestle N/A Yes N/A N/A N/A Yes N/A
Filter Column N/A Yes Yes N/A N/A N/A N/A
Enzyme
Dnase I
Dnase I
Dnase I
Lysozyme
Dnase I
Dnase I
N/A
DNase I
Typical Yield
2-3 µg from
300 µl blood,
25-30 µg from
106 cells
5-30 µg
5-30 µg
40-50 µg from 1x109 bacterial cells
15-20 µg from 5x107
yeast cells
5-30 µg
5-30 µg
Elution Volume 50 µl 50 µl 50 µl 50 µl 50 µl 50 µl 25-50 µl
Time <20 min. <15 min. <20 min. <20 min. <20 min. <30 min. <15 min.

Related RNA Extraction Products

Product Name                       RXNS              Catalogue Number
50, 100, 300
RB050/100/300, RBD050/100/300
50, 100, 300
RT050/100/300, RTD050/100/300
50, 100, 300
RP050/100/300, RPD050/100/300
50, 100, 300
RBB050/100/300, RBBD050/100/300
50, 100, 300
RBY050/100/300, RBYD050/100/300
50
RMI050
GENEzol™ TriRNA Pure Kit 50, 100, 200 GZX050/100/200, GZXD050/100/200
GENEzol™ 96 Well TriRNA Pure Kit 4 x 96, 10 x 96 96GZX04/10