96 Well Blood DNA Kit (96GBP04, 96GBP10)

96 Well Blood Genomic DNA Extraction Kit
96 Well Blood DNA Kit (96GBP04, 96GBP10)
The Presto™ 96 Well Blood Genomic DNA Extraction Kit was designed for high-throughput purification of genomic, mitochondrial and virus DNA from whole blood (fresh blood and frozen blood), plasma, serum, body fluids, lymphocytes and cultured cells. This DNA extraction kit uses protease and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the Presto™ gDNA 96 Well Binding Plate. Contaminants are removed using a Wash Buffer and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The procedure can be completed within 45 minutes without phenol/chloroform extraction or alcohol precipitation. The purified genomic DNA (approximately 20-30 kb) is suitable for use in PCR or other enzymatic reactions.

 

 

Advantages (Cat. # 96GBP04, 96GBP10)

  • Purify 96 wells of pure genomic DNA in 45 minutes!
  • Sample Volume: up to 200 µl of whole blood, plasma, serum, body fluids and up to 5 x 106 lymphocytes or cultured cells per well
  • Yield: up to 6 μg of genomic DNA from 200 µl of whole blood per well 
  • Format: Presto™ gDNA 96 Well Binding Plates
  • Elution Volume: 200 ~ 400 µl
  • Centrifuge or vacuum manifold protocol
  • Storage: dry at room temperature (15-25ºC)

Applications

PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR

Components

  • GB Buffer
  • Protease
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
  • Presto™ gDNA 96 Well Binding Plates
  • 96 Deep Well Plates
  • Microtubes (Racked)
  • Microtubes (8-strip)
  • Caps for Microtubes (8-strip)

Quality Control

The quality of the Presto™ 96 Well Blood Genomic DNA Extraction Kit is tested on a lot-to-lot basis by purifying genomic DNA from 200 μl of whole blood samples. The purified DNA is quantified with a spectrophotometer and analyzed by electrophoresis.
 
Presto™ 96 Well Blood Genomic DNA Extraction Kit Functional Test Data
Figure 1. Genomic DNA was extracted from 200 μl whole human blood samples using the Presto™ 96 Well Genomic DNA Extraction Kit. The purified genomic DNA was eluted in 200 µl of Elution Buffer and 15 µl aliquots of the final sample (chosen from 4 random wells) were analyzed by electrophoresis on a 1% agarose gel. M = Geneaid™ 1 Kb DNA Ladder
Sample
ng/µl
260/280 Yield
1. 200 µl whole blood 27.6 1.81 4.7 µg
2. 200 µl whole blood 28.2 1.75 4.8 µg
3. 200 µl whole blood 32.9 1.74 5.6 µg
4. 200 µl whole blood 25.9 1.81 4.4 µg

 

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