96 Well PCR Cleanup Kit (96DFH04, 96DFH10)

Presto™ 96 Well PCR Cleanup Kits were designed for DNA fragments purification from PCR or other enzymatic reactions using an efficient 96 well binding plate system. This PCR Purification Kit uses chaotropic salt to denature enzymes, allowing DNA fragments to be bound by the glass fiber matrix in each well of the 96 well binding plate. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer, TE or water. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation and the purified DNA is ready for use in subsequent reactions. The Presto™ 96 Well PCR Cleanup Kit is a cost effective, high performance alternative for efficient DNA fragments PCR purification.

Protocol download

Advantages (Cat. # 96DFH04, 96DFH10)

Purify up to 100 µl of PCR products per well in 20 minutes!
High Recovery: up to 95%
Format: Presto™ PCR Cleanup 96 Well Binding Plates
Broad Fragment Size Range: 70 bp–20 kb
Centrifuge or vacuum manifold protocol
Elution Volume: 60 µl from 80 µl elution buffer volume, 40 µl from 60 µl elution buffer volume
Storage: dry at room temperature (15-25ºC)

Applications

PCR, Fluorescent or Radioactive Sequencing, Restriction Enzyme Digestion, DNA Labeling and Ligation

Components

Binding Buffer
Wash Buffer
Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
Presto™ PCR Cleanup 96 Well Binding Plates
0.35 ml Collection Plates
Adhesive Film

Quality Control

The quality of the Presto™ 96 Well PCR Cleanup Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. DNA fragments of various sizes are purified from PCR products and analyzed by electrophoresis.

Presto™ 96 Well PCR Cleanup Kit Interactive Protocol

Presto™ 96 Well PCR Cleanup Kit Sequencing Data

Figure 1. Sequencing data of PCR product (E. coli 16S ribosomal DNA fragment) purified using the Presto™ 96 Well PCR Cleanup Kit.

Gel Extraction and PCR Cleanup Kits Specifications

Specifications	GenepHlow™ Gel Extraction Kit	GenepHlow™ PCR Cleanup Kit	GenepHlow™ Gel/PCR Kit	GenepHlow™ DNA Cleanup Maxi Kit	Presto™ 96 Well PCR Cleanup Kit	Small DNA Fragments Extraction Kit	Presto™ Max Gel/PCR Kit
Cat. #	DFG100 DFG300	DFC100 DFC300	DFH100 DFH300	DFM010 DFM025	96DFH04 96DFH10	DF101 DF301	DFL100 DFL300
Format	Spin Column	Spin Column	Spin Column	Maxi Spin Column	96 Well Plate	Spin Column	Silica Matrix
Binding Capacity	10 µg	10 µg	10 µg	200 µg	10 µg	10 µg	5 µg
Sample	Agarose Gel Slice	PCR Product	Agarose Gel Slice/PCR Product	Agarose Gel Slice/Restriction Enzyme Digest Product	PCR Product	Agarose Gel Slice/PCR Product	Agarose Gel Slice/PCR Product
Fragment Size	70 bp-20 kb	70 bp-20 kb	70 bp-20 kb	100 bp-10 kb	70 bp-20 kb	40-200 bp	100 bp-50 kb
pH Indicator	Yes	Yes	Yes	Yes	N/A	N/A	Yes
Recovery	Up to 90%	Up to 95%	Up to 95%	Up to 80%	Up to 95%	Up to 95%	Up to 95% (<10 kb) Up to 80% (10-50 kb)
Elution Volume	20-50 µl	20-50 µl	20-50 µl	500-1000 µl	60-80 µl	20-50 µl	10-20 µl
Operation Time	20 minutes	10 minutes	<20 minutes	<25 minutes	20 minutes	<20 minutes	<20 minutes

Related Gel Extraction and PCR Cleanup Products

Product Name	RXNS	Catalogue Number
GenepHlow™ Gel Extraction Kit	100, 300	DFG100/300
GenepHlow™ PCR Cleanup Kit	100, 300	DFC100/300
GenepHlow™ Gel/PCR Kit	100, 300	DFH100/300
GenepHlow™ DNA Cleanup Maxi Kit	10, 25	DFM010/25
Presto™ 96 Well PCR Cleanup Kit	4 x 96, 10 x 96	96DFH04/10
Small DNA Fragments Extraction Kit	100, 300	DF101/301
Presto™ Max Gel/PCR Kit	100, 300	DFL100/300
Gel/PCR DNA Fragments Extraction Kit	100, 300	DF100/300
Presto™ 96 Well Gel Extraction Kit	4 x 96, 10 x 96	96DFG04/10