96 Well PCR Cleanup Kit (96DFH04, 96DFH10)

96 Well PCR Cleanup Kit
96 Well PCR Cleanup Kit (96DFH04, 96DFH10)
Presto™ 96 Well PCR Cleanup Kits were designed for DNA fragments purification from PCR or other enzymatic reactions using an efficient 96 well binding plate system. This PCR Purification Kit uses chaotropic salt to denature enzymes, allowing DNA fragments to be bound by the glass fiber matrix in each well of the 96 well binding plate. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer, TE or water. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation and the purified DNA is ready for use in subsequent reactions. The Presto™ 96 Well PCR Cleanup Kit is a cost effective, high performance alternative for efficient DNA fragments PCR purification.
 

Advantages (Cat. # 96DFH04, 96DFH10)

  • Purify up to 100 µl of PCR products per well in 20 minutes!
  • High Recovery: up to 95%
  • Format: Presto™ PCR Cleanup 96 Well Binding Plates
  • Broad Fragment Size Range: 70 bp–20 kb
  • Centrifuge or vacuum manifold protocol
  • Elution Volume: 60 µl from 80 µl elution buffer volume, 40 µl from 60 µl elution buffer volume
  • Storage: dry at room temperature (15-25ºC)

Applications

PCR, Fluorescent or Radioactive Sequencing, Restriction Enzyme Digestion, DNA Labeling and Ligation

Components

  • Binding Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  • Presto™ PCR Cleanup 96 Well Binding Plates
  • 0.35 ml Collection Plates
  • Adhesive Film

Quality Control

The quality of the Presto™ 96 Well PCR Cleanup Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. DNA fragments of various sizes are purified from PCR products and analyzed by electrophoresis.

Presto™ 96 Well PCR Cleanup Kit Interactive Protocol

Presto™ 96 Well PCR Cleanup Kit Sequencing Data

Figure 1. Sequencing data of PCR product (E. coli 16S ribosomal DNA fragment) purified using the Presto™ 96 Well PCR Cleanup Kit.

Gel Extraction and PCR Cleanup Kits Specifications

Specifications
 
Cat. #
DFG100
DFG300
DFC100
DFC300
DFH100
DFH300
DFM010
DFM025
96DFH04
96DFH10
DF101
DF301
DFL100
DFL300
Format
Spin Column
Spin Column
Spin Column
Maxi Spin Column
96 Well Plate
Spin Column
Silica Matrix
Binding Capacity 10 µg 10 µg 10 µg 200 µg 10 µg 10 µg 5 µg
Sample Agarose Gel Slice PCR Product Agarose Gel Slice/PCR Product Agarose Gel Slice/Restriction Enzyme Digest Product PCR Product Agarose Gel Slice/PCR Product Agarose Gel Slice/PCR Product
Fragment Size 70 bp-20 kb 70 bp-20 kb 70 bp-20 kb 100 bp-10 kb 70 bp-20 kb 40-200 bp 100 bp-50 kb
pH Indicator Yes Yes Yes Yes N/A N/A Yes
Recovery Up to 90% Up to 95% Up to 95% Up to 80% Up to 95% Up to 95%
Up to 95% (<10 kb)
Up to 80% (10-50 kb)
Elution Volume 20-50 µl 20-50 µl 20-50 µl 500-1000 µl 60-80 µl 20-50 µl 10-20 µl
Operation Time 20 minutes 10 minutes <20 minutes <25 minutes 20 minutes <20 minutes <20 minutes
Related Gel Extraction and PCR Cleanup Products
Product Name
RXNS
Catalogue Number
100, 300
DFG100/300
100, 300
DFC100/300
100, 300
DFH100/300
10, 25
DFM010/25
4 x 96, 10 x 96
96DFH04/10
100, 300
DF101/301
100, 300
DFL100/300
100, 300
DF100/300
Presto™ 96 Well Gel Extraction Kit
4 x 96, 10 x 96
96DFG04/10