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96 Well Plant DNA Kit (96GPP04, 96GPP10)
The Presto™ 96 Well Plant Genomic DNA Extraction Kit is designed for high-throughput purification of total DNA (including genomic DNA, mitochondrial and chloroplast DNA) from various plant species. Homogenized samples are treated with RNase A then centrifuged to remove cell debris and salt precipitates. In the presence of a binding buffer, genomic DNA in the lysate binds to the glass fiber matrix of the Presto™ 96 Well gDNA Binding Plate. Contaminants are removed using a Wash Buffer and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed within 60 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA (approximately 20-30 kb) is suitable for use in PCR or other enzymatic reactions.
 

 

 

Advantages (Cat. # 96GPP04, 96GPP10)

  • Purify 96 wells of pure genomic DNA from a variety of plant species in 60 minutes!
  • Sample Volume: 100 mg of fresh plant tissue and 25 mg of dried plant tissue per well
  • Yield: up to 30 μg of genomic DNA from 100 mg of fresh plant tissue per well 
  • Format: Presto™ 96 Well gDNA Binding Plates
  • Elution Volume: 200~400 µl
  • Centrifuge or vacuum manifold protocol
  • Storage: dry at room temperature (15-25ºC)

Applications

PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR

Components

  • GP1 Buffer and GPX1 Buffer
  • GP2 Buffer
  • GP3 Buffer
  • RNase A
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
  • Presto™ gDNA 96 Well Binding Plates
  • 96 Deep Well Plates
  • Microtubes (Racked)
  • Microtubes (8-strip)
  • Caps for Microtubes (8-strip)

Quality Control

The quality of the Presto™ 96 Well Plant Genomic DNA Extraction Kit is tested on a lot-to-lot basis by purifying genomic DNA from corn leaf samples. The purified DNA is quantified with a spectrophotometer and analyzed by electrophoresis.
 
Presto™ 96 Well Plant Genomic DNA Extraction Kit Functional Test Data
Figure 1. Genomic DNA was extracted from 50 mg of fresh corn leaf samples using the Presto™ 96 Well Plant Genomic DNA Extraction Kit. The purified genomic DNA was eluted in 200 µl of Elution Buffer and 15 µl aliquots of the final sample (chosen from 3 random wells) were analyzed by electrophoresis on a 1% agarose gel.
M = Geneaid™ 1 Kb DNA Ladder
Sample
ng/µl
260/280260/230Yield
1. Corn Leaf
77.7
1.80
2.02
13.6 µg
2. Corn Leaf
78.8
1.80
2.05
13.8 µg
3. Corn Leaf
76
1.79
1.99
13.3 µg

 
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