Proteinase K

Proteinase K
Proteinase K
Proteinase K is a non-specific serine protease which remains active in detergents and reagents over a broad pH range (4.0-12.5, optimum pH8.0) and is also stable over the temperature range of 25ºC to 65ºC during use. Proteinase K is useful for protein digestion and contamination removal during nucleic acid purification to prepare RNA and high molecular weight DNA for subsequent reactions. Maintaining consistent storage conditions is essential as temperature fluctuations can affect product stability. For optimal performance, prepare Proteinase K just prior to use.

Advantages (Cat. # PK000011, PK000100)  

  • Active in detergents and reagents
  • Broad pH range (4.0-12.5, optimum pH8.0)
  • Active temperature range of 25°C to 65°C during use
  • Lyopholized Proteinase K retains enymatic activity at room temperature for extended periods
  • Effective protein digestion and contamination removal

Applications

Protein digestion and contamination removal during nucleic acid purification to prepare RNA and high molecular weight DNA for subsequent reactions

Storage

Lyophilized Proteinase K should be stored at 2-8ºC for extended periods. Proteinase K solutions are stable for 6 months at 2-8ºC.

Quality Control

The quality of Proteinase K is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system.

Proteinase K Functional Test Data            

Figure 1. 0.5 cm mouse tail samples following incubation at 60ºC for 24 hours. The mouse tail on the right was digested in 20 µl of Proteinase K.

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