96 Well Gel Extraction Kit (96DFG04, 96DFG10)

96 Well Gel Extraction Kit
96 Well Gel Extraction Kit (96DFG04, 96DFG10)
Presto™ 96 Well Gel Extraction Kits were designed to recover or concentrate DNA fragments from agarose gel. QG Buffer (yellow color indicating optimal pH≤7.5) is premixed with a pH indicator to ensure optimal pH, facilitate DNA binding and allow for easy observation of undissolved agarose gel. If pH exceeds the optimal level (>7.5), the color of the solution will appear purple instead of yellow. 3M Sodium Acetate (pH5.0) which is included with the kit, can then be added to the solution to adjust pH and return the color to yellow. Chaotropic salt is used to dissolve agarose gel and denature enzymes while DNA fragments are bound by the glass fiber matrix of the Presto™ Gel Extraction 96 Well Binding Plate. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer, TE or water. The pH indicator, salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation and the purified DNA is ready for use in subsequent reactions.
 

Advantages (Cat. # 96DFG04, 96DFG10)

  • Purify up to 300 mg of agarose gel slices per well in 45 minutes!
  • High Recovery: up to 80%
  • Format: Presto™ Gel Extraction 96 Well Binding Plates
  • Broad Fragment Size Range: 70 bp–20 kb
  • Centrifuge or vacuum manifold protocol
  • Elution Volume: 60 µl from 80 µl elution buffer volume, 40 µl from 60 µl elution buffer volume
  • Storage: dry at room temperature (15-25ºC) for up to 18 months without showing any reduction in performance

Applications

PCR, Fluorescent or Radioactive Sequencing, Restriction Enzyme Digestion, DNA Labeling and Ligation

Components

  • QG Buffer (premixed with pH indicator)
  • 3M Sodium Acetate (pH5.0)
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  • Presto™ Gel Extraction 96 Well Binding Plates
  • 0.35 ml Collection Plates
  • Adhesive Film

Quality Control

The quality of the Presto™ 96 Well Gel Extraction Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. DNA fragments of various sizes are purified from agarose gel and analyzed by electrophoresis.

Presto™ 96 Well Gel Extraction Kit Functional Test Data

Figure 1. Gel slice DNA fragments ranging from 250 bp-10 kb were extracted using the Presto™ 96 Well Gel Extraction Kit (lane 1, 2, 3, 4). The purified DNA from a 60 µl eluate (chosen from 4 random wells) was analyzed by electrophoresis on a 0.8% agarose gel.
M = Geneaid 1 Kb DNA Ladder (control, total DNA = 1100 ng)
Test
DNA Conc.
Yield
Recovery
1
14 ng/µl
840 ng 
76.4%
2
13.9 ng/µl
834 ng
75.8%
3
13.4 ng/µl
804 ng
73.1%
4 14.6 ng/µl 876 ng 79.6%
Optimal pH        pH Too High
Figure 2. A pH indicator is premixed with QG Buffer to ensure optimal pH, facilitate DNA binding and allow for easy observation of undissolved agarose gel. If pH exceeds the optimal level (>7.5), the color of the solution will appear purple instead of yellow. 3M Sodium Acetate (pH5.0), which is included with the kit, can then be added to the solution to adjust pH and return the color to yellow.

Gel Extraction and PCR Cleanup Kits Specifications

Specifications
 
Cat. #
DFG100
DFG300
DFC100
DFC300
DFH100
DFH300
DFM010
DFM025
96DFG04
96DFG10
DF101
DF301
DFL100
DFL300
Format
Spin Column
Spin Column
Spin Column
Maxi Spin Column
96 Well Plate
Spin Column
Silica Matrix
Binding Capacity 10 µg 10 µg 10 µg 200 µg 10 µg 10 µg 5 µg
Sample Agarose Gel Slice PCR Product Agarose Gel Slice/PCR Product Agarose Gel Slice/Restriction Enzyme Digest Product Agarose Gel Slice Agarose Gel Slice/PCR Product Agarose Gel Slice/PCR Product
Fragment Size 70 bp-20 kb 70 bp-20 kb 70 bp-20 kb 100 bp-10 kb 70 bp-20 kb 40-200 bp 100 bp-50 kb
pH Indicator Yes Yes Yes Yes N/A N/A Yes
Recovery Up to 90% Up to 95% Up to 95% Up to 80% Up to 80% Up to 95%
Up to 95% (<10 kb)
Up to 80% (10-50 kb)
Elution Volume 20-50 µl 20-50 µl 20-50 µl 500-1000 µl 60-80 µl 20-50 µl 10-20 µl
Operation Time 20 minutes 10 minutes <20 minutes <25 minutes 45 minutes <20 minutes <20 minutes

Related Gel Extraction and PCR Cleanup Products

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Catalogue Number
100, 300
DFG100/300
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4 x 96, 10 x 96
96DFH04/10
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Presto™ 96 Well Gel Extraction Kit 4 x 96, 10 x 96 96DFG04/10
GenepHlow™ DNA Cleanup Midi Kit 100, 300 DFI100/300