96 Well DNA Bacteria Advanced Kit (96GBBA04, 96GBBA10)

96 Well Bacteria Genomic DNA Extraction Kit
96 Well DNA Bacteria Advanced Kit (96GBBA04, 96GBBA10)
The Presto™ 96 Well DNA Bacteria Advanced Kit is optimized for high-throughput genomic DNA purification from Gram (-) negative and Gram (+) positive bacterial cells. After directly adding bacteria samples into beadbeating microtubes pre-filled with zirconia/ceramic beads and lysis buffer, cells are efficiently lysed via bead beating instrument without enzymatic digestion. The cell lysate is then mixed with a binding buffer and the genomic DNA is bound by the membrane in the 96 well plate, followed by wash and elution. The entire procedure can be completed within 90 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA (approximately 20-30 kb) is suitable for use in PCR or other enzymatic reactions.
 
Advantages (Cat. # 96GBBA04, 96GBBA10)
  • 96 well bead beating microtubes pre-filled with zirconia/ceramic beads
  • Enzymatic digestion is not required!
  • High-throughput bacteria DNA extraction
  • High Yield: up to 5 μg of genomic DNA per well
  • High Purity gDNA: A260/A280 >1.9
  • DNA Size: 20-40 kb
  • Samples: Gram (+) Positive and Gram (-) Negative bacterial cells (up to 5 x 108)
  • 96 Well Binding Plates: glass fiber membrane optimized for genomic DNA extraction (sterilised to remove bacteria contamination)
  • Elution volume: 60-100 µl
  • Storage: dry at room temperature (15-25°C)
Applications
 
PCR, Restriction Endonuclease Digestions, Array, Southern Blots and various other enzymatic reactions
 
Components
  • GT Buffer
  • PR Buffer
  • GB Buffer
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
  • Presto™ gDNA 96 Well Binding Plates
  • Beadbeating Microtubes (Racked)
  • Beadbeating Microtubes (8-strip)
  • Caps for Microtubes (8-strip)
  • 96 Deep Well Plates
  • Adhesive Film
  • 0.35 ml Collection Plate

Quality Control

The quality of the Presto™ 96 Well DNA Bacteria Advanced Kit is tested on a lot-to-lot basis by isolating DNA from Escherichia coli (5×108) culture. 5 µl from a 100 µl eluate of purified DNA is analyzed by electrophoresis on a 1% agarose gel.
 
Presto™ 96 Well DNA Bacteria Advanced Kit Functional Test Data
Sample
ng/µl
260/280
Yield
Test 1
49.2
1.80
3.9 µg
Test 2
56.2
1.79
4.5 µg
Test 3
55.8
1.80
4.5 µg
Test 4 22.4 1.87 1.8 µg
Test 5 19.9 1.84 1.6 µg
Test 6 20.4 1.80 1.6 µg

Figure 1. Genomic DNA was extracted from 20 mg of Escherichia coli and Bacillus subtilis pellet respectively using the Presto™ 96 Well DNA Bacteria Advanced Kit. 5 µl aliquots of purified genomic DNA from a 80 µl eluate was analyzed by electrophoresis on a 0.8% agarose gel.
M = Geneaid™ 1 Kb DNA Ladder
1-4: 5 x 108 Escherichia coli cells, 5-8: 5 x 108 Bacillus subtilis cells    
   

Genomic DNA Extraction Kits Specifications
Specifications
Genomic DNA Mini/Maxi Kit Plant
Cat. #
GS100
GS300
GB100
GB300
GT050
GT100
GT300
GP100
GPM010
GPM025
GBB100/101
GBB300/301
GBYB100
GBYB300
GMB100
GMB300
GSK100
GSK300
Format
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Reagent System
Spin Column Spin Column
Binding Capacity 50 µg 50 µg 50 µg

50 µg (Mini)

500 µg (Maxi)

50 µg N/A 50 µg 50 µg
Sample 200 µl blood 300 µl blood 30 mg animal tissue Mini: 100 mg plant tissue 1x109 bacterial cells 2x108 yeast cells 1-100 µl blood buccal swabs
25 mg animal tissue 200 µl buffy coat   Maxi: 1 g plant tissue 200 µl blood   FTA® Cards  
1x107 cultured cells 1x107 cultured cells     1 ml urine, plasma   1 ml urine  
hair
2x108 yeast/fungus         1-100 µl saliva  
Micropestle N/A N/A Yes N/A N/A N/A N/A N/A
Filter Column N/A N/A N/A Yes N/A N/A N/A Yes
Enzyme
Proteinase
K
N/A
Proteinase
K
N/A
Lysozyme Proteinase K
Lyticase or
Zymolase
Proteinase K
Proteinase K
Typical Yield
5 µg from
200 µl blood
4-10 µg from
300 µl blood
10-40 µg from
20 mg tissue
3-5 µg from 100 mg Arabidopsis leaf (Mini)
37 µg from 1x109
E. coli
10-15 µg from 2x108 S. cerevisiae
cells
300 ng from 6 mm
blood spots
2 µg per swab
10-40 µg from
20 mg tissue
 
 
80 µg from 500 mg Lemon leaf (Maxi)
10-15 µg from 1x109
Bacillus subtilis
     
Elution Volume 30-100 µl 30-200 µl 30-200 µl

30-200 µl (Mini)

1 ml (Maxi)

30-200 µl 50-100 µl 30-100 µl 50-100 µl
Operation Time <20 minutes <25 minutes 30 minutes <30 minutes <30 minutes <40 minutes <20 minutes <20 minutes
Related Genomic DNA Extraction Products
Product Name
RXNS
Catalogue Number
100, 300 
GS100/300
100, 300
GB100/300
10, 25
GPM010/25
10, 25
GDM10/25
50, 100, 300
GT050/100/300
100
GP100
100
GMB100/300
100
GSK100/300
100, 300
GBYB100/300
100, 300
GBB100/101/300/301
4 x 96, 10 x 96
96GBP04/10
Presto™ 96 Well Plant Genomic DNA Extraction Kit
4 x 96, 10 x 96
96GPP04/10
4 x 96, 10 x 96
96GBB04/10