96 Well Bacteria DNA Kit (96GBB04, 96GBB10)

96 Well Bacteria Genomic DNA Extraction Kit
96 Well Bacteria DNA Kit (96GBB04, 96GBB10)
The Presto™ 96 Well gDNA Bacteria Kit is optimized for high-throughput genomic and viral DNA purification from Gram (-) negative and Gram (+) positive bacterial cells. Gram+ Buffer, when combined with Lysozyme, will efficiently lyse bacterial cell walls consisting of the peptidoglycan layer. Proteinase K and chaotropic salt are used to further lyse cells and degrade protein, allowing DNA to easily bind to the glass fiber matrix of the binding plate. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. Phenol/chloroform extraction or alcohol precipitation is not required and the purified genomic DNA is ready for use in a variety of downstream applications. 
Advantages (Cat. # 96GBB04, 96GBB10)
  • High-throughput bacteria DNA extraction
  • High Yield: up to 5 μg of genomic DNA per well
  • High Purity gDNA: A260/A280 >1.9
  • DNA Size: 20-40 kb
  • Samples: Gram (+) Positive and Gram (-) Negative bacterial cells (up to 5 x 108)
  • Convenient: Includes Gram+ Buffer for preparing Lysozyme solutions to speed up sample preparation when purifying DNA from Gram (+) positive bacteria
  • 96 Well Binding Plates: glass fiber membrane optimized for genomic DNA extraction (sterilised to remove bacteria contamination)
  • Elution volume: 100-200 µl
  • Storage: dry at room temperature (15-25°C), Lysozyme is shipped at room temperature and should be stored at -20°C for extended periods
Applications
 
PCR, Restriction Endonuclease Digestions, Array, Southern Blots and various other enzymatic reactions
 
Components
  • Proteinase K
  • Gram+ Buffer
  • GT Buffer
  • GB Buffer
  • W1 Buffer
  • Wash Buffer
  • Lysozyme
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
  • Presto™ gDNA 96 Well Binding Plates
  • Microtubes (Racked)
  • Microtubes (8-strip)
  • Caps for Microtubes (8-strip)
  • 96 Deep Well Plates
  • Adhesive Film

Quality Control

The quality of the Presto™ 96 Well gDNA Bacteria Kit is tested on a lot-to-lot basis by isolating DNA from Escherichia coli (5×108) culture. 5 µl from a 100 µl eluate of purified DNA is analyzed by electrophoresis on a 1% agarose gel.
 
Presto™ 96 Well gDNA Bacteria Kit Functional Test Data (Gram (-) Negative Bacteria
Figure 1. Genomic DNA was extracted from bacteria cells using the Presto™ 96 Well gDNA Bacteria Kit. The purified genomic DNA was eluted in 100 µl of Elution Buffer and 10 µl aliquots of the final sample (chosen from 6 random wells) were analyzed by electrophoresis on a 1% agarose gel. 
M = Geneaid™ 1 Kb DNA Ladder
1-3: 5 x 108 Escherichia coli cells, 4-6: 5 x 108 Bacillus subtilis cells    
Sample
ng/µl
260/280
Yield
Test 1
49.2
1.80
3.9 µg
Test 2
56.2
1.79
4.5 µg
Test 3
55.8
1.80
4.5 µg
Test 4 22.4 1.87 1.8 µg
Test 5 19.9 1.84 1.6 µg
Test 6 20.4 1.80 1.6 µg
Genomic DNA Extraction Kits Specifications
Specifications
Genomic DNA Mini/Maxi Kit Plant
Cat. #
GS100
GS300
GB100
GB300
GT050
GT100
GT300
GP100
GPM010
GPM025
GBB100/101
GBB300/301
GBYB100
GBYB300
GMB100
GMB300
GSK100
GSK300
Format
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Reagent System
Spin Column Spin Column
Binding Capacity 50 µg 50 µg 50 µg

50 µg (Mini)

500 µg (Maxi)

50 µg N/A 50 µg 50 µg
Sample 200 µl blood 300 µl blood 30 mg animal tissue Mini: 100 mg plant tissue 1x109 bacterial cells 2x108 yeast cells 1-100 µl blood buccal swabs
25 mg animal tissue 200 µl buffy coat   Maxi: 1 g plant tissue 200 µl blood   FTA® Cards  
1x107 cultured cells 1x107 cultured cells     1 ml urine, plasma   1 ml urine  
hair
2x108 yeast/fungus         1-100 µl saliva  
Micropestle N/A N/A Yes N/A N/A N/A N/A N/A
Filter Column N/A N/A N/A Yes N/A N/A N/A Yes
Enzyme
Proteinase
K
N/A
Proteinase
K
N/A
Lysozyme Proteinase K
Lyticase or
Zymolase
Proteinase K
Proteinase K
Typical Yield
5 µg from
200 µl blood
4-10 µg from
300 µl blood
10-40 µg from
20 mg tissue
3-5 µg from 100 mg Arabidopsis leaf (Mini)
37 µg from 1x109
E. coli
10-15 µg from 2x108 S. cerevisiae
cells
300 ng from 6 mm
blood spots
2 µg per swab
10-40 µg from
20 mg tissue
 
 
80 µg from 500 mg Lemon leaf (Maxi)
10-15 µg from 1x109
Bacillus subtilis
     
Elution Volume 30-100 µl 30-200 µl 30-200 µl

30-200 µl (Mini)

1 ml (Maxi)

30-200 µl 50-100 µl 30-100 µl 50-100 µl
Operation Time <20 minutes <25 minutes 30 minutes <30 minutes <30 minutes <40 minutes <20 minutes <20 minutes
Related Genomic DNA Extraction Products
Product Name
RXNS
Catalogue Number
100, 300 
GS100/300
100, 300
GB100/300
10, 25
GPM010/25
10, 25
GDM10/25
50, 100, 300
GT050/100/300
100
GP100
100
GMB100/300
100
GSK100/300
100, 300
GBYB100/300
100, 300
GBB100/101/300/301
4 x 96, 10 x 96
96GBP04/10
Presto™ 96 Well Plant Genomic DNA Extraction Kit
4 x 96, 10 x 96
96GPP04/10
4 x 96, 10 x 96
96GBB04/10