Bacteria Genomic DNA Kit (GBB100/101, GBB300/301)

Bacteria Genomic DNA Extraction Kit
Bacteria Genomic DNA Kit (GBB100/101, GBB300/301)
The Presto™ Mini gDNA Bacteria Kit is a Bacteria genomic DNA extraction kit optimized for genomic and viral DNA purification from Gram negative bacterial cells and Gram positive bacterial cells. This Bacteria Genomic DNA Extraction Kit includes Gram+ Buffer and Lysozyme and when combined, will efficiently lyse bacterial cell walls consisting of the peptidoglycan layer. Chaotropic salt is used to lyse cells and degrade protein, allowing DNA to easily bind to the glass fiber matrix of the genomic DNA spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed within 30 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA, with approximately 20-40 kb, is suitable for use in PCR or other enzymatic reactions
Advantages (Cat. # GBB100, GBB101, GBB300, GBB301)
  • Purify genomic DNA within 30 minutes!
  • High Yield: up to 40 μg (1 x 109 Escherichia coli) and 15 µg (1 x 109 Bacillus subtilis)
  • High Purity gDNA: A260/A280 >1.9
  • DNA Size: 20-40 kb
  • Samples: Gram (+) Positive and Gram (-) Negative bacterial cells (up to 1 x 109), 200 µl of blood and biological fluids such as plasma and urine
  • Convenient: Includes Gram+ Buffer for preparing Lysozyme solutions to speed up sample preparation when purifying DNA from Gram (+) positive bacteria
  • Spin Columns: glass fiber membrane optimized for genomic DNA extraction (sterilised to remove bacteria contamination)
  • Elution volume: 30-200 µl
  • Storage: dry at room temperature (15-25°C)
Applications
 
PCR, Restriction Endonuclease Digestions, Array, Southern Blots and various other enzymatic reactions
 
Components
  • Proteinase K
  • Gram+ Buffer
  • GT Buffer
  • GB Buffer
  • W1 Buffer
  • Wash Buffer
  • Lysozyme
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
  • GD Columns
  • 2 ml Collection Tubes

Quality Control

The quality of the Presto™ Mini gDNA Bacteria Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. The purified genomic DNA from Escherichia coli cells (up to 1 x 109), (up to 40 µg with an A260/A280 ratio of >2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.
 
Presto™ Mini gDNA Bacteria Kit Functional Test Data Gram (-) Negative Bacteria
Figure 1. Genomic DNA (approximately 30 kb) was extracted using the Presto™ Mini Bacteria Kit. An Escherichia coli (1×109) culture (OD600=2, 1 ml) was harvested by centrifugation at 16,000 x g for 1 minute. A 5 µl aliquot of purified genomic DNA from a 100 µl eluate was analyzed by electrophoresis on a 1% agarose gel. M = Geneaid 1 Kb DNA Ladder
Sample
Yield
260/280
260/230
Test 1
38.16 µg
2.05
2.26
Test 2
33.88 µg
2.05
2.27
Test 3
39.02 µg
2.07
2.29
Presto™ Mini gDNA Bacteria Kit Functional Test Data Gram (+) Positive Bacteria 
Figure 2. Genomic DNA (approximately 30 kb) was extracted using the Presto™ Mini Bacteria Kit. A Bacillus subtilis (1×109) culture (OD600=2 1 ml) was harvested by centrifugation at 16,000 x g for 1 minute. A 5 µl aliquot of purified genomic DNA from a 100 µl eluate was analyzed by electrophoresis on a 1% agarose gel. M = Geneaid 1 Kb DNA Ladder
Sample
Yield
260/280
260/230
Test 1
12.28 µg
1.96
2.28
Test 2
12.08 µg
1.97
2.30
Test 3
12.56 µg
1.96
2.29
Genomic DNA Extraction Kits Specifications
Specifications
Genomic DNA Mini/Maxi Kit Plant
Cat. #
GS100
GS300
GB100
GB300
GT050
GT100
GT300
GP100
GPM010
GPM025
GBB100/101
GBB300/301
GBYB100
GBYB300
GMB100
GMB300
GSK100
GSK300
Format
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column Spin Column Spin Column
Binding Capacity 50 µg 50 µg 50 µg

50 µg (Mini)

500 µg (Maxi)

50 µg N/A 50 µg 50 µg
Sample 200 µl blood 300 µl blood 30 mg animal tissue Mini: 100 mg plant tissue 1x109 bacterial cells 2x108 yeast cells 1-100 µl blood buccal swabs
25 mg animal tissue 200 µl buffy coat   Maxi: 1 g plant tissue 200 µl blood   FTA® Cards  
1x107 cultured cells 1x107 cultured cells     1 ml urine, plasma   1 ml urine  
hair
2x108 yeast/fungus         1-100 µl saliva  
Micropestle N/A N/A Yes N/A N/A N/A N/A N/A
Filter Column N/A N/A N/A Yes N/A N/A N/A Yes
Enzyme
Proteinase
K
N/A
Proteinase
K
N/A
Lysozyme Proteinase K
N/A
Proteinase K
Proteinase K
Typical Yield
5 µg from
200 µl blood
4-10 µg from
300 µl blood
10-40 µg from
20 mg tissue
3-5 µg from 100 mg Arabidopsis leaf (Mini)
37 µg from 1x109
E. coli
10 µg from 2x108 S. cerevisiae
cells
300 ng from 6 mm
blood spots
2 µg per swab
10-40 µg from
20 mg tissue
 
 
80 µg from 500 mg Lemon leaf (Maxi)
10-15 µg from 1x109
Bacillus subtilis
     
Elution Volume 30-100 µl 30-200 µl 30-200 µl

30-200 µl (Mini)

1 ml (Maxi)

30-200 µl 30-200 µl 30-100 µl 50-100 µl
Operation Time <20 minutes <25 minutes 30 minutes <30 minutes <30 minutes <40 minutes <20 minutes <20 minutes
Related Genomic DNA Extraction Products
Product Name
RXNS
Catalogue Number
100, 300 
GS100/300
100, 300
GB100/300
10, 25
GPM010/25
10, 25
GDM10/25
50, 100, 300
GT050/100/300
100
GP100
100
GMB100/300
100
GSK100/300
100, 300
GBYB100/300
100, 300
GBB100/101/300/301
4 x 96, 10 x 96
96GBP04/10
Presto™ 96 Well Plant Genomic DNA Extraction Kit
4 x 96, 10 x 96
96GPP04/10