Blood/Cell DNA Mini Kit (GB100, GB300)

Genomic DNA Extraction Kit Blood and Cultured Cell
Blood/Cell DNA Mini Kit (GB100, GB300)

The Genomic DNA Mini Kit (Blood/Cultured Cell) is a genomic DNA extraction kit optimized for genomic, mitochondrial and virus DNA purification from whole blood, buffy coat and cultured cell samples. This Genomic DNA Extraction Kit uses RBC Lysis Buffer and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the genomic DNA spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. Phenol/chloroform extraction or alcohol precipitation is not required and the purified genomic DNA (approximately 20-30 kb) is suitable for use in PCR or various other enzymatic reactions. For RNA purification from blood and cultured cell samples, the Total RNA Mini Kit (Blood/CC) is also available.

 

 

 

Advantages (Cat. # GB100, GB300)
  • Purify genomic DNA within 25 minutes!
  • High Yield, High Quality Genomic DNA: 4-10 μg of pure genomic DNA (A260/A280 = 1.8-2.0) from 300 µl of whole blood
  • Variety of Samples: up to 300 µl of whole blood, up to 200 µl of buffy coat, up to 1 x 107 cultured cells, up to 2 x 108 yeast and other fungus species
  • Spin Columns: glass fiber membrane optimized for genomic DNA extraction
  • Elution Volume: 30-100 µl
  • Storage: dry at room temperature (15-25°C)
Applications
 
PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR
 
Components
  • RBC Lysis Buffer
  • GT Buffer
  • GB Buffer
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  • GD Columns
  • 2 ml Collection Tubes
Quality Control
 
The quality of the Genomic DNA Mini Kit (Blood/Cultured Cell) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Genomic DNA from 300 μl of whole human blood is extracted using the Genomic DNA Mini Kit (Blood/Cultured Cell). The purified DNA (4-10 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.

Genomic DNA Mini Kit (Blood/Cultured Cell) Functional Test Data

Figure 1. Genomic DNA (20-30 kb) was extracted from 300 µl of whole blood using the Genomic DNA Mini Kit (Blood/Cultured Cell). The purified DNA was eluted in 200 µl of Elution Buffer and 15 µl aliquots of the final sample were analyzed by electrophoresis on a 1% agarose gel. M = Geneaid 1 Kb DNA Ladder

Sample Yield 260/280
1. 300 µl 8.07 µg 1.87
2. 300 µl 8.18 µg 1.87
3. 300 µl 8.19 µg 1.87
Genomic DNA Extraction Kits Specifications
Specifications
Genomic DNA Mini/Maxi Kit Plant
Cat. #
GS100
GS300
GB100
GB300
GT050
GT100
GT300
GP100
GPM010
GPM025
GBB100/101
GBB300/301
GBY100
GBY300
GMB100
GMB300
GSK100
GSK300
Format
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Reagent System
Spin Column Spin Column
Binding Capacity 50 µg 50 µg 50 µg

50 µg (Mini)

500 µg (Maxi)

50 µg N/A 50 µg 50 µg
Sample 200 µl blood 300 µl blood 30 mg animal tissue Mini: 100 mg plant tissue 1x109 bacterial cells 2x108 yeast cells 1-100 µl blood buccal swabs
25 mg animal tissue 200 µl buffy coat   Maxi: 1 g plant tissue 200 µl blood   FTA® Cards  
1x107 cultured cells 1x107 cultured cells     1 ml urine, plasma   1 ml urine  
hair
2x108 yeast/fungus         1-100 µl saliva  
Micropestle N/A N/A Yes N/A N/A N/A N/A N/A
Filter Column N/A N/A N/A Yes N/A N/A N/A Yes
Enzyme
Proteinase
K
N/A
Proteinase
K
N/A
Lysozyme Proteinase K
Lyticase or
Zymolase
Proteinase K
Proteinase K
Typical Yield
5 µg from
200 µl blood
4-10 µg from
300 µl blood
10-40 µg from
20 mg tissue
3-5 µg from 100 mg Arabidopsis leaf (Mini)
37 µg from 1x109
E. coli
10-15 µg from 2x108 S. cerevisiae
cells
300 ng from 6 mm
blood spots
2 µg per swab
10-40 µg from
20 mg tissue
 
 
80 µg from 500 mg Lemon leaf (Maxi)
10-15 µg from 1x109
Bacillus subtilis
     
Elution Volume 30-100 µl 30-200 µl 30-200 µl

30-200 µl (Mini)

1 ml (Maxi)

30-200 µl 50-100 µl 30-100 µl 50-100 µl
Operation Time <20 minutes <25 minutes 30 minutes <30 minutes <30 minutes <40 minutes <20 minutes <20 minutes
Related Genomic DNA Extraction Products
Product Name
RXNS
Catalogue Number
100, 300 
GS100/300
100, 300
GB100/300
10, 25
GPM010/25
10, 25
GDM10/25
50, 100, 300
GT050/100/300
100
GP100
100
GMB100/300
100
GSK100/300
100, 300
GBY100/300
100, 300
GBB100/101/300/301
4 x 96, 10 x 96
96GBP04/10
Presto™ 96 Well Plant Genomic DNA Extraction Kit
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