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Blood DNA Midi Kit (GDI100)
The Genomic DNA Midi Kit Blood is optimized for DNA purification (including genomic, mitochondrial and viral DNA) from up to 3 ml of fresh blood using an efficient mini spin column and column extension tube system. This DNA Extraction Kit uses Protease to reduce hemoglobin contamination while chaotropic salt and detergents are used to lyse cells and degrade protein, allowing the DNA to bind to the glass fiber matrix of the genomic DNA spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed 30 minutes without phenol/chloroform extraction or alcohol precipitation. Purified DNA, with approximately 20-30 kb, is suitable for use in PCR or other enzymatic reactions.
Advantages (Cat. # GDI100)
  • Binding Capacity: up to 50 μg
  • Large Sample Size: up to 3 ml of blood compared to 200 µl using the mini kit
  • Genomic glass fiber mini spin column and Column Extension Tubes for increased sample volume and yield
  • Elution Volume: 30-100 µl
  • Storage: dry at room temperature (15-25°C)
  • Operation time: within 30 minutes
Applications
 
PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR
 
Components
  • GSB Buffer
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  • Protease
  • GS Columns and Column Extension Tubes
  • 2 ml Collection Tubes
Quality Control
 
The quality of the Genomic DNA Midi Kit (Blood) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Genomic DNA from 3 ml of whole human blood is extracted using the Genomic DNA Midi Kit (Blood). The purified DNA is quantified with a spectrophotometer and analyzed by electrophoresis.