DNA Extraction > Genomic DNA Purification

gSYNC™ DNA Extraction Kit

gSYNC™ DNA Extraction Kit
gSYNC™ DNA Extraction Kit

The gSYNC™ DNA Extraction Kit is a genomic DNA extraction kit optimized for genomic, mitochondrial and virus DNA purification from whole blood (fresh blood and frozen blood), serum, plasma, buffy coat, body fluids, cultured cells, tissue, rodent tails, ear punches, formalin-fixed paraffin-embedded tissue (FFPE), amniotic fluid, hair and insects in one convenient kit. This DNA extraction kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed in less than 20 minutes without phenol/chloroform extraction or alcohol precipitation. Purified DNA, with approximately 20-30 kb, is suitable for use in PCR or other enzymatic reactions.

 

Protocol download

 

 

 

Advantages (Cat. # GS100, GS300)

  • Purify genomic DNA within 20 minutes!
  • Variety of Samples: 50-200 μl of whole blood, plasma serum buffy coat, up to 25 mg of tissue, rodent tails, ear punches, insects, up to 1 x 107 cultured cells, up to 25 mg of formalin-fixed-paraffin-embedded tissue, up to 15 ml of amniotic fluid, hair
  • High Yield: up to 5 µg from whole blood
  • High Quality: A260/A280 = 1.8-2.0
  • NO manual homogenization of tissue samples required
  • Genomic DNA glass fiber spin columns (green membrane optimized for genomic DNA extraction)
  • Storage: dry at room temperature (15-25°C) for up to 1 year

Applications

PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR

Components

  • GST Buffer
  • GSB Buffer
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  • Proteinase K
  • GD Columns
  • 2 ml Collection Tubes

Quality Control

The quality of the gSYNC™ DNA Extraction Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Genomic DNA from 200 μl of whole human blood is extracted using the gSYNC™ DNA Extraction Kit. The purified DNA (5 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.

gSYNC™ DNA Extraction Kit Functional Test Data
Figure 1. Genomic DNA from 50, 100 and 200 µl whole blood samples was extracted using the gSYNC™ DNA Extraction Kit. 10 µl from 100 µl eluates of purified genomic DNA was analyzed by electrophoresis on a 0.8% agarose gel.
1-3 = 50 µl whole blood sample, 4-6 = 100 µl whole blood sample, 7-9 = 200 µl whole blood sample
M = Geneaid 1 Kb DNA Ladder

 
Sample Yield 260/280
50 µl 1.54 µg 1.85
100 µl 2.70 µg 1.87
200 µl 5.56 µg 1.90

gSYNC™ DNA Extraction Kit Interactive Protocol Procedure
Video 1. A step by step demonstrational video of the gSYNC™ DNA Extraction Kit. Genomic DNA from 200 μl of whole human blood is extracted using the gSYNC™ DNA Extraction Kit. The purified DNA (5 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.

Related Genomic DNA Extraction Products

Product Name RXNS Catalogue Number
gSYNC™ DNA Extraction Kit (Tissue/Blood) 100, 300  GS100/300
100, 300
GB100/300
25
GDI25
10, 25
GDM10/25
50, 100, 300
GT050/100/300
100
GP100
10, 25
GPM10/25
Geneius™ Micro gDNA Extraction Kit 100 GMB100/300
Presto™ Buccal Swab gDNA Extraction Kit 100 GSK100/300
100, 300
GBY100/300
100, 300
GBB100/300
4 x 96, 10 x 96
GBP04/10
4 x 96, 10 x 96
GPP04/10

 

                                 

 

 

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