Tissue/Blood DNA Mini Kit (GS100, GS300)

Blood and Tissue DNA Extraction Kit
Tissue/Blood DNA Mini Kit (GS100, GS300)

The gSYNC™ DNA Extraction Kit is a genomic DNA extraction kit optimized for genomic, mitochondrial and virus DNA purification from whole blood (fresh blood and frozen blood), serum, plasma, buffy coat, body fluids, cultured cells, tissue, rodent tails, ear punches, formalin-fixed paraffin-embedded tissue (FFPE), amniotic fluid, hair, insects and sperm in one convenient kit. This DNA extraction kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed in less than 20 minutes without phenol/chloroform extraction or alcohol precipitation. Purified DNA, with approximately 20-30 kb, is suitable for use in PCR or other enzymatic reactions.

 

 

 

 

Advantages (Cat. # GS100, GS300)

  • Purify genomic DNA within 20 minutes!
  • Variety of Samples: 50-200 μl of whole blood, plasma, serum, buffy coat, up to 25 mg of tissue, rodent tails, ear punches, insects, up to 1 x 107 cultured cells, up to 25 mg of formalin-fixed-paraffin-embedded tissue, up to 15 ml of amniotic fluid, hair, up to 100 µl of sperm
  • High Yield: µg from 200 µl whole blood
  • High Quality DNA: A260/A280 = 1.8-2.0
  • Spin Columns: glass fiber membrane optimized for genomic DNA extraction
  • Elution Volume: 30-100 µl
  • Storage: dry at room temperature (15-25°C)

Applications

PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR

Components

  • GST Buffer
  • GSB Buffer
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  • Proteinase K
  • GS Columns
  • 2 ml Collection Tubes

Quality Control

The quality of the gSYNC™ DNA Extraction Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Genomic DNA from 200 μl of whole human blood is extracted using the gSYNC™ DNA Extraction Kit. The purified DNA (5 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.

gSYNC™ DNA Extraction Kit Functional Test Data
Figure 1. Genomic DNA from 50, 100 and 200 µl whole blood samples was extracted using the gSYNC™ DNA Extraction Kit. 10 µl from 100 µl eluates of purified genomic DNA was analyzed by electrophoresis on a 0.8% agarose gel.
1-3 = 50 µl whole blood sample, 4-6 = 100 µl whole blood sample, 7-9 = 200 µl whole blood sample
M = Geneaid 1 Kb DNA Ladder
Sample Yield 260/280
50 µl 1.54 µg 1.85
100 µl 2.70 µg 1.87
200 µl 5.56 µg 1.90

gSYNC™ DNA Extraction Kit Interactive Protocol Procedure

  Video 1. A step by step demonstrational video of the gSYNC™ DNA Extraction Kit. Genomic DNA from 200 μl of whole human blood is extracted using the gSYNC™ DNA Extraction Kit. The purified DNA (5 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.

Genomic DNA Extraction Kits Specifications

Specifications
Genomic DNA Mini/Maxi Kit Plant
Cat. #
GS100
GS300
GB100
GB300
GT050
GT100
GT300
GP100
GPM010
GPM025
GBB100/101
GBB300/301
GBY100
GBYB300
GMB100
GMB300
GSK100
GSK300
Format
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column Spin Column
Binding Capacity 50 µg 50 µg 50 µg

50 µg (Mini)

500 µg (Maxi)

50 µg 50 µg 50 µg 50 µg
Sample 200 µl blood 300 µl blood 30 mg animal tissue Mini: 100 mg plant tissue 1x109 bacterial cells 2x108 yeast cells 1-100 µl blood buccal swabs
25 mg animal tissue 200 µl buffy coat   Maxi: 1 g plant tissue 200 µl blood   FTA® Cards  
1x107 cultured cells 1x107 cultured cells     1 ml urine, plasma   1 ml urine  
hair
2x108 yeast/fungus         1-100 µl saliva  
Micropestle N/A N/A Yes N/A N/A N/A N/A N/A
Filter Column N/A N/A N/A Yes N/A N/A N/A Yes
Enzyme
Proteinase
K
N/A
Proteinase
K
N/A
Lysozyme Proteinase K
N/A
Proteinase K
Proteinase K
Typical Yield
5 µg from
200 µl blood
4-10 µg from
300 µl blood
10-40 µg from
20 mg tissue
3-5 µg from 100 mg Arabidopsis leaf (Mini)
37 µg from 1x109
E. coli
10 µg from 2x108 S. cerevisiae
cells
300 ng from 6 mm
blood spots
2 µg per swab
10-40 µg from
20 mg tissue
 
 
80 µg from 500 mg Lemon leaf (Maxi)
10-15 µg from 1x109
Bacillus subtilis
     
Elution Volume 30-100 µl 30-200 µl 30-200 µl

30-200 µl (Mini)

1 ml (Maxi)

30-200 µl 30-200 µl 30-100 µl 50-100 µl
Operation Time <20 minutes <25 minutes 30 minutes <30 minutes <30 minutes <40 minutes <20 minutes <20 minutes

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