DNA Extraction > Genomic DNA Purification

Presto™ Buccal Swab gDNA Extraction Kit

Buccal Swab gDNA Extraction Kit
Presto™ Buccal Swab gDNA Extraction Kit

The Presto™ Buccal Swab gDNA Extraction Kit provides an efficient method for purifying DNA (including genomic, mitochondrial and viral DNA) from buccal cells. This DNA Extraction Kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. S3 Powder is included with the kit to improve the efficiency of DNA binding to the spin column membrane. Contaminants are removed using W1 Buffer and Wash Buffer (containing ethanol). The purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. High quality genomic DNA can be purified in 20 minutes without phenol/chloroform extraction or alcohol precipitation, with an average DNA yield of 2 μg per buccal swab. Purified DNA, with approximately 20-30 kb, is suitable for use in PCR or other enzymatic reactions.

Protocol download
Advantages (Cat. # GSK100, GSK300)
  • Purify genomic DNA from buccal cell swabs in 20 minutes!
  • High Yield: up to 2 μg of pure genomic DNA per buccal swab
  • High Purity gDNA: A260/A280 = 1.8-2.0
  • DNA Size: 20-30 kb
  • Genomic DNA glass fiber spin column (green membrane optimized for genomic DNA purification from buccal cells)
  • Elution volume: 50-100 µl
  • Storage: dry at room temperature (15-25°C) for up to 1 year
Applications
 
PCR, Restriction Endonuclease Digestions, Array, Southern Blots and various other enzymatic reactions
 
Components
  • S1 Buffer
  • S2 Buffer
  • S3 Powder
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
  • Proteinase K
  • GD Columns
  • Filter Columns
  • 2 ml Collection Tubes

Quality Control

The quality of the Presto™ Buccal Swab gDNA Extraction Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. The purified genomic DNA (2 µg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.
 
Presto™ Buccal Swab gDNA Extraction Kit Funtional Test Data
Figure 1. Genomic DNA from 3 individual cotton buccal swab samples was extracted using the Presto™ Buccal Swab gDNA Extraction Kit. The purified genomic DNA was analyzed by electrophoresis on a 0.8% agarose gel.

M = Geneaid 1 Kb DNA Ladder

Sample
DNA Conc.
260/280
Yield
Test 1
52.20 ng/µl
1.86
1.83 µg
Test 2
34.80 ng/µl
1.90
1.22 µg
Test 3
50.90 ng/µl
1.92
1.78 µg
Figure 2. The purified genomic DNA was used as a template for amplifying partial human 5S ribosomal DNA (91 bp) by PCR. The PCR products were analyzed on a 0.8% agarose gel.
 
M = Geneaid 100 bp DNA Ladder
Related Genomic DNA Extraction Products
Product Name                                                                          RXNS                          Catalogue Number
Presto™ Buccal Swab gDNA Extraction Kit
 100, 300 GSK100/300
100, 300 GMB100/300
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GBB100/300
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GB100/300
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GBP04/10
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