Presto™ Buccal Swab gDNA Extraction Kit

Buccal Swab Genomic DNA Extraction Kit
Presto™ Buccal Swab gDNA Extraction Kit

The Presto™ Buccal Swab gDNA Extraction Kit provides an efficient method for purifying DNA (including genomic, mitochondrial and viral DNA) from buccal cells. This DNA Extraction Kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Carrier RNA is included with the kit to improve the efficiency of DNA binding to the spin column membrane. Contaminants are removed using W1 Buffer and Wash Buffer (containing ethanol). The purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. High quality genomic DNA can be purified in 20 minutes without phenol/chloroform extraction or alcohol precipitation, with an average DNA yield of 2 μg per buccal swab. Purified DNA, with approximately 20-30 kb, is suitable for use in PCR or other enzymatic reactions.

Protocol download
Advantages (Cat. # GSK100, GSK300)
  • Purify genomic DNA from buccal cell swabs in 20 minutes!
  • High Yield: up to 2 μg of pure genomic DNA per buccal swab
  • High Purity gDNA: A260/A280 = 1.8-2.0
  • DNA Size: 20-30 kb
  • Spin Columns: glass fiber membrane optimized for genomic DNA extraction
  • Elution volume: 50-100 µl
  • Storage: dry at room temperature (15-25°C) for up to 1 year, Proteinase K should be stored at 2-8°C and Carrier RNA should be stored at -20°C for extended periods
Applications
 
PCR, Restriction Endonuclease Digestions, Array, Southern Blots and various other enzymatic reactions
 
Components
  • S1 Buffer
  • S2 Buffer
  • Carrier RNA
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
  • Proteinase K
  • GD Columns
  • Filter Columns
  • 2 ml Collection Tubes

Quality Control

The quality of the Presto™ Buccal Swab gDNA Extraction Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. The purified genomic DNA (2 µg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.
 
Presto™ Buccal Swab gDNA Extraction Kit Funtional Test Data
Figure 1. Genomic DNA from 3 individual cotton buccal swab samples was extracted using the Presto™ Buccal Swab gDNA Extraction Kit. The purified genomic DNA was analyzed by electrophoresis on a 0.8% agarose gel.

M = Geneaid 1 Kb DNA Ladder

Sample
DNA Conc.
260/280
Yield
Test 1
52.20 ng/µl
1.86
1.83 µg
Test 2
34.80 ng/µl
1.90
1.22 µg
Test 3
50.90 ng/µl
1.92
1.78 µg
Figure 2. The purified genomic DNA was used as a template for amplifying partial human 5S ribosomal DNA (91 bp) by PCR. The PCR products were analyzed on a 0.8% agarose gel.
 
M = Geneaid 100 bp DNA Ladder
Genomic DNA Extraction Kits Specifications
Specifications
Cat. #
GS100
GS300
GB100
GB300
GT050
GT100
GT300
GP100
GBB100/101
GBB300/301
GBY100
GBY300
GMB100
GMB300
GSK100
GSK300
Format
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Reagent System
Spin Column Spin Column
Binding Capacity 50 µg 50 µg 50 µg 50 µg 50 µg N/A 50 µg 50 µg
Sample 200 µl blood 300 µl blood 30 mg animal tissue 100 mg plant tissue 1x109 bacterial cells 2x108 yeast cells 1-100 µl blood buccal swabs
25 mg animal tissue 200 µl buffy coat     200 µl blood   FTA® Cards  
1x107 cultured cells 1x107 cultured cells     1 ml urine, plasma   1 ml urine  
hair
2x108 yeast/fungus         1-100 µl saliva  
Micropestle N/A N/A Yes N/A N/A N/A N/A N/A
Filter Column N/A N/A N/A Yes N/A N/A N/A Yes
Enzyme
Proteinase
K
N/A
Proteinase
K
N/A
Lysozyme
Lyticase or
Zymolase
Proteinase K
Proteinase K
Typical Yield
5 µg from
200 µl blood
4-6 µg from
200 µl blood
10-40 µg from
20 mg tissue
3-5 µg from 100 mg Arabidopsis leaf
37 µg from 1x109
E. coli
10-15 µg from 2x108 S. cerevisiae
cells
300 ng from 6 mm
blood spots
2 µg per swab
10-40 µg from
20 mg tissue
 
 
20-25 µg from 100 mg Nicotiana leaf
10-15 µg from 1x109
Bacillus subtilis
     
Elution Volume 30-100 µl 30-200 µl 30-200 µl 30-200 µl 30-200 µl 50-100 µl 30-100 µl 50-100 µl
Operation Time <20 minutes <25 minutes 30 minutes <30 minutes <30 minutes <40 minutes <20 minutes <20 minutes
Related Genomic DNA Extraction Products
Product Name
RXNS
Catalogue Number
100, 300
GS100/300
100, 300
GB100/300
25
GDI25
10, 25
GDM10/25
50, 100, 300
GT050/100/300
100
GP100
100
GMB100/300
100
GSK100/300
100, 300
GBY100/300
100, 300
GBB100/101/300/301
Presto™ 96 Well Genomic DNA Extraction Kit
4 x 96, 10 x 96
96GB004/10
Presto™ 96 Well Genomic DNA Extraction Kit (Plant)
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