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Presto™ Sperm DNA Extraction Kit (GSP100/GSP300)
This miniprep DNA extraction kit was designed for sperm DNA purification from up to 100 µl of semen. Proteinase K and a unique sperm lysis buffer are used to lyse sperm cells and degrade protein, allowing DNA to efficiently bind to the glass fiber matrix of the spin column. Contaminants are removed using a wash buffer and the purified DNA is eluted by a low salt elution buffer, TE or water. Following cell lysis, the procedure can be completed in as little as 10 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA is suitable for use in PCR or a variety of other enzymatic reactions. 

Advantages (Cat. # GSP100, GSP300)

  • Samples: up to 100 μl of animal semen
  • Binding Capacity: 50 μg
  • Includes Sperm Lysis Buffer for efficient sperm cell lysis
  • Elution Volume: 30-200 μl
  • Storage: dry at room temperature (15-25°C)

Applications

PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR

Components

  • Sperm Lysis Buffer
  • GSB Buffer
  • Proteinase K
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer
  • GS Columns
  • 2 ml Collection Tubes

Quality Control

The quality of the Presto™ Sperm DNA Extraction Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Total DNA is isolated from 100 µl of animal semen samples, quantified with a spectrophotometer and analyzed by electrophoresis.