Tissue Genomic DNA Mini Kit (GT050, GT100, GT300)

Genomic DNA Extraction Kit Tissue
Tissue Genomic DNA Mini Kit (GT050, GT100, GT300)

The Genomic DNA Mini Kit (Tissue) is a genomic DNA Extraction Kit optimized for genomic, mitochondrial and virus DNA purification from a variety of animal tissue and insects. This genomic DNA extraction kit includes Micropestles to ensure efficient homogenization of tissue samples and to shorten the time in the Lysis Step. Proteinase K and chaotropic salt are used to lyse cells and degrade protein, allowing DNA to be easily bound by the glass fiber matrix of the genomic DNA spin column. Once any contaminants have been removed, using a Wash Buffer (containing ethanol), the purified DNA is eluted by a low salt Elution Buffer, TE or water and is ready for use in a variety of downstream applications such as PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR. For RNA extraction from tissue samples, the Total RNA Mini Kit (Tissue) is also available.

 

 

 

Advantages (Cat. # GT050, GT100, GT300)
  • Purify genomic DNA from tissue samples in 30 minutes!
    High Yield: 10-20 µg (0.5 cm mouse tail), 20-50 µg (20 mg mouse kidney)
  • Sample Size: up to 30 mg of tissue (tailsnips, liver, kidney, brain, earpunches, insects etc.)
  • Spin Columns: glass fiber membrane optimized for genomic DNA extraction
  • Elution Volume: 30-100 µl
  • Provided Micropestles are certified DNase and RNase-free
  • Storage: dry at room temperature (15-25°C)
Applications
 
PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR

Components

  • Micropestle
  • GT Buffer
  • GBT Buffer
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  • Proteinase K
  • GS Columns
  • 2 ml Collection Tubes

Quality Control

The quality of the Genomic DNA Mini Kit (Tissue) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Genomic DNA from a 20 mg mouse liver sample is extracted using the Genomic DNA Mini Kit (Tissue). The purified DNA (10 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.

Genomic DNA Mini Kit (Tissue) Functional Test Data

Figure 1. Genomic DNA from a variety of tissue samples was extracted using the Genomic DNA Mini Kit (Tissue). The purified genomic DNA (20-30 kb) was EcoRI digested and analyzed by electrophoresis on a 1% agarose gel.
M =  Geneaid 1 Kb DNA Ladder
Mouse Tissue Tissue Size Total Yield
Tail 0.5 cm 10-20 µg
Liver 20 mg 10-20 µg
Brain 20 mg 10-20 µg
Lung 20 mg 5-10 µg
Muscle 20 mg 5-10 µg
Kidney 20 mg 20-50 µg
Genomic DNA Extraction Kit Specifications
Specifications
Genomic DNA Mini/Maxi Kit Plant
Cat. #
GS100
GS300
GB100
GB300
GT050
GT100
GT300
GP100
GPM010
GPM025
GBB100/101
GBB300/301
GBYB100
GBYB300
GMB100
GMB300
GSK100
GSK300
Format
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column Spin Column
Binding Capacity 50 µg 50 µg 50 µg

50 µg (Mini)

500 µg (Maxi)

50 µg N/A 50 µg 50 µg
Sample 200 µl blood 300 µl blood 30 mg animal tissue Mini: 100 mg plant tissue 1x109 bacterial cells 2x108 yeast cells 1-100 µl blood buccal swabs
25 mg animal tissue 200 µl buffy coat   Maxi: 1 g plant tissue 200 µl blood   FTA® Cards  
1x107 cultured cells 1x107 cultured cells     1 ml urine, plasma   1 ml urine  
hair
2x108 yeast/fungus         1-100 µl saliva  
Micropestle N/A N/A Yes N/A N/A N/A N/A N/A
Filter Column N/A N/A N/A Yes N/A N/A N/A Yes
Enzyme
Proteinase
K
N/A
Proteinase
K
N/A
Lysozyme Proteinase K
N/A
Proteinase K
Proteinase K
Typical Yield
5 µg from
200 µl blood
4-10 µg from
300 µl blood
10-40 µg from
20 mg tissue
3-5 µg from 100 mg Arabidopsis leaf (Mini)
37 µg from 1x109
E. coli
10 µg from 2x108 S. cerevisiae
cells
300 ng from 6 mm
blood spots
2 µg per swab
10-40 µg from
20 mg tissue
 
 
80 µg from 500 mg Lemon leaf (Maxi)
10-15 µg from 1x109
Bacillus subtilis
     
Elution Volume 30-100 µl 30-200 µl 30-200 µl

30-200 µl (Mini)

1 ml (Maxi)

30-200 µl 30-200 µl 30-100 µl 50-100 µl
Operation Time <20 minutes <25 minutes 30 minutes <30 minutes <30 minutes <40 minutes <20 minutes <20 minutes
Related Genomic DNA Extraction Products
Product Name
RXNS
Catalogue Number
100, 300 
GS100/300
100, 300
GB100/300
10, 25
GPM010/25
10, 25
GDM10/25
50, 100, 300
GT050/100/300
100
GP100
100
GMB100/300
100
GSK100/300
100, 300
GBYB100/300
100, 300
GBB100/101/300/301
4 x 96, 10 x 96
96GBP04/10
Presto™ 96 Well Plant Genomic DNA Extraction Kit
4 x 96, 10 x 96
96GPP04/10