Yeast Genomic DNA Kit (GBY100, GBY300)

Yeast and Fungus DNA Extraction Kit
Yeast Genomic DNA Kit (GBY100, GBY300)
The Presto™ Mini gDNA Yeast Kit offers a simple and gentle reagent DNA precipitation method for isolating high molecular weight genomic, mitochondrial or viral DNA from Saccharomyces cerevisiae and a variety of other yeast and fungus species. This highly versatile solution based system offers a convenient procedure with minimal hands on time. The provided Sorbitol Buffer, when combined with zymolase or lyticase, will efficiently lyse yeast and other fungus species cell walls consisting of chitin and polysaccharides. Purified DNA (A260/A280 = 1.8-2.0), is suitable for use in PCR or other enzymatic reactions.
Advantages (Cat. # GBY100, GBY300)
  • Purify genomic DNA from (up to 2 x 108) yeast and other fungus species using a simple and gentle 3 buffer system
  • Simple and gentle reagent DNA precipitation method
  • Convenient premixed Sorbitol Buffer!
  • Elution volume: 50-100 µl 
  • Time: 40 minutes
  • Storage: dry at room temperature (15-25°C) for up to 2 years without showing any reduction in performance

Applications

DNA archiving, PCR, AFLP, RFLP/PADP, Southern Blotting, Real-time PCR

Components

  • Sorbitol Buffer 
  • Cell Lysis Buffer
  • Protein Removal Buffer
  • DNA Hydration Buffer

Quality Control

The quality of the Presto™ Mini gDNA Yeast Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. The purified genomic DNA (A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.

Presto™ Mini gDNA Yeast Kit Funtional Test Data

Figure 1. Genomic DNA (approximately 30 kb) was extracted using the Presto™ Mini gDNA Yeast Kit. Saccharomyces cerevisiae (2×108) was harvested by centrifugation at 5,000 x g for 10 minutes. A 15 µl aliquot of purified genomic DNA from a 100 µl eluate was analyzed by electrophoresis on a 1% agarose gel. M = Geneaid 1 Kb DNA Ladder

Test DNA Conc. 260/280 260/230 Yield
1 115.5 µg/ml 1.91 1.87 11.6 µg
2 142.9 µg/ml 1.92 2.01 14.3 µg
3 137.1 µg/ml 1.91 1.97 13.7 µg
Genomic DNA Extraction Kits Specifications
Specifications
Genomic DNA Mini/Maxi Kit Plant
Cat. #
GS100
GS300
GB100
GB300
GT050
GT100
GT300
GP100
GPM010
GPM025
GBB100/101
GBB300/301
GBY100
GBY300
GMB100
GMB300
GSK100
GSK300
Format
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Reagent System
Spin Column Spin Column
Binding Capacity 50 µg 50 µg 50 µg

50 µg (Mini)

500 µg (Maxi)

50 µg N/A 50 µg 50 µg
Sample 200 µl blood 300 µl blood 30 mg animal tissue Mini: 100 mg plant tissue 1x109 bacterial cells 2x108 yeast cells 1-100 µl blood buccal swabs
25 mg animal tissue 200 µl buffy coat   Maxi: 1 g plant tissue 200 µl blood   FTA® Cards  
1x107 cultured cells 1x107 cultured cells     1 ml urine, plasma   1 ml urine  
hair
2x108 yeast/fungus         1-100 µl saliva  
Micropestle N/A N/A Yes N/A N/A N/A N/A N/A
Filter Column N/A N/A N/A Yes N/A N/A N/A Yes
Enzyme
Proteinase
K
N/A
Proteinase
K
N/A
Lysozyme Proteinase K
Lyticase or
Zymolase
Proteinase K
Proteinase K
Typical Yield
5 µg from
200 µl blood
4-10 µg from
300 µl blood
10-40 µg from
20 mg tissue
3-5 µg from 100 mg Arabidopsis leaf (Mini)
37 µg from 1x109
E. coli
10-15 µg from 2x108 S. cerevisiae
cells
300 ng from 6 mm
blood spots
2 µg per swab
10-40 µg from
20 mg tissue
 
 
80 µg from 500 mg Lemon leaf (Maxi)
10-15 µg from 1x109
Bacillus subtilis
     
Elution Volume 30-100 µl 30-200 µl 30-200 µl

30-200 µl (Mini)

1 ml (Maxi)

30-200 µl 50-100 µl 30-100 µl 50-100 µl
Operation Time <20 minutes <25 minutes 30 minutes <30 minutes <30 minutes <40 minutes <20 minutes <20 minutes
Related Genomic DNA Extraction Products
Product Name
RXNS
Catalogue Number
100, 300 
GS100/300
100, 300
GB100/300
10, 25
GPM010/25
10, 25
GDM10/25
50, 100, 300
GT050/100/300
100
GP100
100
GMB100/300
100
GSK100/300
100, 300
GBY100/300
100, 300
GBB100/101/300/301
4 x 96, 10 x 96
96GBP04/10
Presto™ 96 Well Plant Genomic DNA Extraction Kit
4 x 96, 10 x 96
96GPP04/10