Yeast Genomic DNA Kit (GBYB100, GBYB300)

Yeast DNA Extraction Kit | Saccharomyces cerevisiae DNA Extraction Kit
Yeast Genomic DNA Kit (GBYB100, GBYB300)
The Presto™ Mini gDNA Yeast Kit was designed for rapid isolation of genomic DNA from cultured yeast and other fungus species. The yeast/fungus cells are directly added into beadbeating tubes containing ceramic beads and lysis buffer. Samples are efficiently lysed without the use of degestive enzymes (such as Lyticase or Zymolase) using a bead beating instrument or a standard vortex. The cell lysate is then mixed with Binding Buffer and the genomic DNA is bound by the GD Column. The column is then washed and the DNA is eluted with Elution Buffer. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 40 minutes. The purified genomic DNA is ready for use in PCR, restriction enzyme digestion, and sequencing reactions. 
Advantages (Cat. # GBYB100, GBYB300)
  • Purify genomic DNA from (up to 2 x 108) yeast and other fungus species
  • Genomic DNA Yield: up to 10 µg of genomic DNA from 2 x 10Saccharomyces cerevisiae 
  • Format: beadbeating tubes and spin columns
  • Elution volume: 30-200 µl 
  • Time: 40 minutes
  • Storage: dry at room temperature (15-25°C)

Applications

PCR, restricion enzyme digestion and sequencing reactions

Components

  • GT Buffer 
  • PR Buffer
  • GB Buffer
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer
  • RNase A (50 mg/m)
  • GD Columns
  • Beadbeating Tubes
  • 2 ml Collection Tubes

Quality Control

The quality of the Presto™ Mini gDNA Yeast DNA  Kit is tested on a lot-to-lot basis by isolating genomic DNA from 2 x108 Saccharomyces cerevisiae cells. Following the purification process, greater than 8 μg of genomic DNA is obtained and the A260/A280 ratio is between 1.7-2.0. The purified genomic DNA is analyzed by electrophoresis.

Geneaid™ Yeast DNA Extraction Kit Funtional Test Data

Figure 1. Genomic DNA was extracted using the Presto™ Mini gDNA Yeast DNA Extraction Kit. Saccharomyces cerevisiae (50 mg, 100 mg, 200 mg) was harvested by centrifugation at 5,000 x g for 10 minutes. 5 µl aliquots of purified genomic DNA from a 100 µl eluate were analyzed by electrophoresis on a 1% agarose gel. 
M = Geneaid 1 Kb DNA Ladder
Test DNA Conc. 260/280 260/230 Yield
1
21.2 ng/µl
1.89
2.09
2.1 µg
2
22.2 ng/µl
1.90
2.05
2.2 µg
3
37.5 ng/µl
1.91
2.18
3.8 µg
4
40.7 ng/µl
1.90
2.05
4.1 µg
5
75.3 ng/µl
1.86
2.07
7.5 µg
6
84.9 ng/µl
1.87
2.11
8.5 µg
Genomic DNA Extraction Kits Specifications
Specifications
Genomic DNA Mini/Maxi Kit Plant
Presto™ Mini gDNA Yeast Kit
Cat. #
GS100
GS300
GB100
GB300
GT050
GT100
GT300
GP100
GPM010
GPM025
GBB100/101
GBB300/301
GBYB100
GBYB300
GMB100
GMB300
GSK100
GSK300
Format
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column Spin Column
Binding Capacity 50 µg 50 µg 50 µg

50 µg (Mini)

500 µg (Maxi)

50 µg 50 µg 50 µg 50 µg
Sample 200 µl blood 300 µl blood 30 mg animal tissue Mini: 100 mg plant tissue 1x109 bacterial cells 2x108 yeast cells 1-100 µl blood buccal swabs
25 mg animal tissue 200 µl buffy coat   Maxi: 1 g plant tissue 200 µl blood   FTA® Cards  
1x107 cultured cells 1x107 cultured cells     1 ml urine, plasma   1 ml urine  
hair
2x108 yeast/fungus         1-100 µl saliva  
Micropestle N/A N/A Yes N/A N/A N/A N/A N/A
Filter Column N/A N/A N/A Yes N/A N/A N/A Yes
Enzyme
Proteinase
K
N/A
Proteinase
K
N/A
Lysozyme Proteinase K
N/A
Proteinase K
Proteinase K
Typical Yield
5 µg from
200 µl blood
4-10 µg from
300 µl blood
10-40 µg from
20 mg tissue
3-5 µg from 100 mg Arabidopsis leaf (Mini)
37 µg from 1x109
E. coli
10 µg from 2x108 S. cerevisiae
cells
300 ng from 6 mm
blood spots
2 µg per swab
10-40 µg from
20 mg tissue
 
 
80 µg from 500 mg Lemon leaf (Maxi)
10-15 µg from 1x109
Bacillus subtilis
     
Elution Volume 30-100 µl 30-200 µl 30-200 µl

30-200 µl (Mini)

1 ml (Maxi)

30-200 µl 50-100 µl 30-100 µl 50-100 µl
Operation Time <20 minutes <25 minutes 30 minutes <30 minutes <40 minutes <40 minutes <20 minutes <20 minutes
Related Genomic DNA Extraction Products
Product Name
RXNS
Catalogue Number
100, 300 
GS100/300
100, 300
GB100/300
10, 25
GPM010/25
10, 25
GDM10/25
50, 100, 300
GT050/100/300
100
GP100
100
GMB100/300
100
GSK100/300
Presto™ Mini gDNA Yeast Kit
100, 300
GBYB100/300
100, 300
GBB100/101/300/301
4 x 96, 10 x 96
96GBP04/10
Presto™ 96 Well Plant Genomic DNA Extraction Kit
4 x 96, 10 x 96
96GPP04/10