Plant Genomic DNA Maxi Kit (GPM010, GPM025)

Genomic DNA Extraction Maxiprep Kit Plant
Plant Genomic DNA Maxi Kit (GPM010, GPM025)

The Genomic DNA Maxi Kit (Plant) is a genomic DNA extraction maxiprep kit optimized for genomic DNA, mitochondrial DNA and chloroplast DNA purification from plant tissue. This DNA extraction kit processes plant samples by grinding in liquid nitrogen followed by lysis buffer incubation. The lysate is treated with RNase A then filtered to remove cell debris and salt precipitates. In the presence of the binding buffer, coupled with chaotropic salt, genomic DNA in the lysate binds to the glass fiber matrix of the maxiprep spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. DNA phenol extraction or alcohol precipitation is not required, and the entire procedure can be completed in less than 50 minutes.

 

 

 

Advantages (Cat. # GPM010, GPM025)

  • Purify genomic DNA in less than 50 minutes!
  • Binding Capacity: 500 µg
  • Sample Size: up to 1 g (fresh plant tissue), up to 250 mg (dry plant tissue)
  • Maxiprep Spin Columns: glass fiber membrane optimized for genomic DNA extraction
  • Elution Volume: 200 µl ~ 2 ml
  • Storage: dry at room temperature (15-25ºC)

Applications

PCR, Real-time PCR, Southern Blotting and RFLP

Components

  • GPX1 Buffer (optimized for samples with high polysaccharide content)
  • GP1, GP2, GP3 Buffers
  • RNase A
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  • Filter Column
  • GD Maxi Column
  • Collection Tube

Quality Control

The quality of the Genomic DNA Maxi Kit (Plant) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Genomic DNA is extracted from 500 mg fresh young leaf samples. The purified genomic DNA (>80 µg) is quantified with a spectrophotometer and analyzed by electrophoresis.

Genomic DNA Maxi Kit (Plant) Functional Test Data

Figure 1. Genomic DNA (approximately 30 kb) was extracted using the Genomic DNA Maxi Kit (Plant). 0.5 g of fresh lemon leaves and 0.5 g of fresh coffee leaves were ground to a fine powder followed by DNA extraction. 2 µl aliquots from a 1 ml eluate were analyzed by electrophoresis on a 1% agarose gel.  
M = Geneaid 1 Kb DNA ladder
Sample DNA Conc. 260/280 Yield
1. Lemon Leaf 80.6 ng/µl 1.81 80.6 µg
2. Lemon Leaf 82.2 ng/µl 1.80 82.2 µg
3. Coffee Leaf 37.3 ng/µl 1.79 37.3 µg
4. Coffee Leaf 36.4 ng/µl 1.75 36.4 µg
Genomic DNA Extraction Kits Specifications
Specifications
Genomic DNA Mini/Maxi Kit Plant
Cat. #
GS100
GS300
GB100
GB300
GT050
GT100
GT300
GP100
GPM010
GPM025
GBB100/101
GBB300/301
GBYB100
GBYB300
GMB100
GMB300
GSK100
GSK300
Format
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Reagent System
Spin Column Spin Column
Binding Capacity 50 µg 50 µg 50 µg

50 µg (Mini)

500 µg (Maxi)

50 µg N/A 50 µg 50 µg
Sample 200 µl blood 300 µl blood 30 mg animal tissue Mini: 100 mg plant tissue 1x109 bacterial cells 2x108 yeast cells 1-100 µl blood buccal swabs
25 mg animal tissue 200 µl buffy coat   Maxi: 1 g plant tissue 200 µl blood   FTA® Cards  
1x107 cultured cells 1x107 cultured cells     1 ml urine, plasma   1 ml urine  
hair
2x108 yeast/fungus         1-100 µl saliva  
Micropestle N/A N/A Yes N/A N/A N/A N/A N/A
Filter Column N/A N/A N/A Yes N/A N/A N/A Yes
Enzyme
Proteinase
K
N/A
Proteinase
K
N/A
Lysozyme Proteinase K
Lyticase or
Zymolase
Proteinase K
Proteinase K
Typical Yield
5 µg from
200 µl blood
4-10 µg from
300 µl blood
10-40 µg from
20 mg tissue
3-5 µg from 100 mg Arabidopsis leaf (Mini)
37 µg from 1x109
E. coli
10-15 µg from 2x108 S. cerevisiae
cells
300 ng from 6 mm
blood spots
2 µg per swab
10-40 µg from
20 mg tissue
 
 
80 µg from 500 mg Lemon leaf (Maxi)
10-15 µg from 1x109
Bacillus subtilis
     
Elution Volume 30-100 µl 30-200 µl 30-200 µl

30-200 µl (Mini)

1 ml (Maxi)

30-200 µl 50-100 µl 30-100 µl 50-100 µl
Operation Time <20 minutes <25 minutes 30 minutes <30 minutes <30 minutes <40 minutes <20 minutes <20 minutes
Related Genomic DNA Extraction Products
Product Name
RXNS
Catalogue Number
100, 300 
GS100/300
100, 300
GB100/300
10, 25
GPM010/25
10, 25
GDM10/25
50, 100, 300
GT050/100/300
100
GP100
100
GMB100/300
100
GSK100/300
100, 300
GBYB100/300
100, 300
GBB100/101/300/301
4 x 96, 10 x 96
96GBP04/10
Presto™ 96 Well Plant Genomic DNA Extraction Kit
4 x 96, 10 x 96
96GPP04/10