Geneius™ Micro gDNA Extraction Kit

Micro Genomic DNA Extraction Kit
Geneius™ Micro gDNA Extraction Kit

The Geneius™ Micro gDNA Extraction Kit provides an efficient method for purifying DNA (including genomic and mitochondrial DNA) from small volumes of whole blood, dried blood spots and urine. This DNA Extraction Kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. S3 Powder is included with this DNA extraction kit to improve the efficiency of DNA binding to the spin column membrane. Contaminants are removed using W1 Buffer and Wash Buffer (containing ethanol). The purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed in as little as 20 minutes without phenol/chloroform extraction or alcohol precipitation, with an average DNA yield of 300 ng from 6 mm dried blood spots. Purified DNA, with approximately 20-30 kb, is suitable for use in PCR or other enzymatic reactions.
 

Protocol download
Advantages (Cat. # GMB100, GMB300)
  • Purify genomic DNA from small volume blood samples, dried blood spots and urine
  • Time: in as little as 20 minutes
  • High Yield: 300 ng of pure genomic DNA from 6 mm blood spots
  • High Purity gDNA: A260/A280 = 1.8-2.0
  • DNA Size: 20-30 kb
  • Spin Columns: glass fiber membrane optimized for genomic DNA extraction
  • Elution volume: 50-100 µl
  • Storage: dry at room temperature (15-25°C) for up to 1 year
Applications
 
PCR, Restriction Endonuclease Digestions, Array, Southern Blots and various other enzymatic reactions
 
Components
  • S1 Buffer
  • S2 Buffer
  • S3 Powder
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
  • Proteinase K
  • GD Columns
  • 2 ml Collection Tubes

Quality Control

The quality of the Geneius™ Micro gDNA Extraction Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. The purified genomic DNA (300 ng with an A260/A280 ratio of 1.8-2.0) from 10 µl blood samples  is quantified with a spectrophotometer and checked by electrophoresis.
 
Genomic DNA Extraction Kits Specifications
Specifications
Cat. #
GS100
GS300
GB100
GB300
GT050
GT100
GT300
GP100
GBB100/101
GBB300/301
GBY100
GBY300
GMB100
GMB300
GSK100
GSK300
Format
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Reagent System
Spin Column Spin Column
Binding Capacity 50 µg 50 µg 50 µg 50 µg 50 µg N/A 50 µg 50 µg
Sample 200 µl blood 300 µl blood 30 mg animal tissue 100 mg plant tissue 1x109 bacterial cells 2x108 yeast cells 1-100 µl blood buccal swabs
25 mg animal tissue 200 µl buffy coat         dried blood spots  
1x107 cultured cells 1x107 cultured cells         1 ml urine  
hair
2x108 yeast/fungus            
Micropestle N/A N/A Yes N/A N/A N/A N/A N/A
Filter Column N/A N/A N/A Yes N/A N/A N/A Yes
Enzyme
Proteinase
K
N/A
Proteinase
K
N/A
Lysozyme Proteinase K
Lyticase or
Zymolase
Proteinase K
Proteinase K
Typical Yield
5 µg from
200 µl blood
4-6 µg from
200 µl blood
10-40 µg from
20 mg tissue
3-5 µg from 100 mg Arabidopsis leaf
37 µg from 1x109
E. coli
10-15 µg from 2x108 S. cerevisiae
cells
300 ng from 6 mm
blood spots
2 µg per swab
10-40 µg from
20 mg tissue
 
 
20-25 µg from 100 mg Nicotiana leaf
10-15 µg from 1x109
Bacillus subtilis
     
Elution Volume 30-100 µl 30-200 µl 30-200 µl 30-200 µl 30-200 µl 50-100 µl 50-100 µl 50-100 µl
Operation Time <20 minutes <25 minutes 30 minutes <30 minutes <30 minutes <40 minutes <20 minutes <20 minutes
Related Genomic DNA Extraction Products
Product Name
RXNS
Catalogue Number
100, 300
GS100/300
100, 300
GB100/300
25
GDI25
10, 25
GDM10/25
50, 100, 300
GT050/100/300
100
GP100
100
GMB100/300
100
GSK100/300
100, 300
GBY100/300
100, 300
GBB100/101/300/301
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