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Dry Blood Spot DNA Kit (GMB100, GMB300)

The Geneius™ Micro gDNA Extraction Kit provides an efficient method for purifying DNA (including genomic and mitochondrial DNA) from small volumes of whole blood, dried blood spots (Whatman® FTA® Cards), urine and saliva. This DNA Extraction Kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Carrier RNA is included with this DNA extraction kit to improve the efficiency of DNA binding to the spin column membrane. Contaminants are removed using W1 Buffer and Wash Buffer (containing ethanol). The purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed in as little as 20 minutes without phenol/chloroform extraction or alcohol precipitation, with an average DNA yield of 150-200 ng from 6 mm dried blood spots. Purified DNA, with approximately 20-30 kb, is suitable for use in PCR or other enzymatic reactions.
 

Advantages (Cat. # GMB100, GMB300)
  • Purify genomic DNA from small volume blood samples (1 µl), dried blood spots (Whatman® FTA® Cards), urine, saliva
  • Time: in as little as 20 minutes
  • High Yield: Up to 300 ng of pure genomic DNA per 6 mm diameter punch from Whatman® FTA® Cards (dried blood spots)
  • High Purity gDNA: A260/A280 = 1.8-2.0
  • DNA Size: 20-30 kb
  • Spin Columns: glass fiber membrane optimized for genomic DNA extraction
  • Elution volume: 30-100 µl
  • Storage: dry at room temperature (15-25°C)  
Applications
 
PCR, Restriction Endonuclease Digestions, Array, Southern Blots and various other enzymatic reactions
 
Components
  • S1 Buffer
  • S2 Buffer
  • Carrier RNA
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
  • Proteinase K
  • GD Columns
  • 2 ml Collection Tubes

Quality Control

The quality of the Geneius™ Micro gDNA Extraction Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. The purified genomic DNA (150-200 ng with an A260/A280 ratio of 1.8-2.0) from 10 µl blood samples is quantified with a spectrophotometer and analyzed by electrophoresis.
 
Real-Time PCR Data
Figure 1. Genomic DNA was extracted from blood samples (1–64 µl) using the Geneius™ Micro gDNA Extraction Kit. The purified DNA was eluted in 30 µl of Elution Buffer followed by a Real-time PCR assay performed with a 3 μl aliquot as template, primers (designed to amplify the ACTB gene), and Fast SYBR Green PCR Master Mix using the StepOnePlus™ Real-Time PCR system (Applied Biosystems). The results confirm the efficiency of DNA extraction from as low as 1 µl blood samples.
 
Genomic DNA Extraction Kits Specifications
Specifications
Genomic DNA Mini/Maxi Kit Plant
Cat. #
GS100
GS300
GB100
GB300
GT050
GT100
GT300
GP100
GPM010
GPM025
GBB100/101
GBB300/301
GBYB100
GBYB300
GMB100
GMB300
GSK100
GSK300
Format
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Spin ColumnSpin Column
Binding Capacity50 µg50 µg50 µg

50 µg (Mini)

500 µg (Maxi)

50 µgN/A50 µg50 µg
Sample200 µl blood300 µl blood30 mg animal tissue Mini: 100 mg plant tissue 1x109 bacterial cells2x108 yeast cells1-100 µl bloodbuccal swabs
25 mg animal tissue 200 µl buffy coat Maxi: 1 g plant tissue200 µl blood FTA® Cards 
1x107 cultured cells1x107 cultured cells  1 ml urine, plasma 1 ml urine 
hair
2x108 yeast/fungus    1-100 µl saliva 
MicropestleN/AN/AYesN/AN/AN/AN/AN/A
Filter ColumnN/AN/AN/AYesN/AN/AN/AYes
Enzyme
Proteinase
K
N/A
Proteinase
K
N/A
Lysozyme Proteinase K
N/A
Proteinase K
Proteinase K
Typical Yield
5 µg from
200 µl blood
4-10 µg from
300 µl blood
10-40 µg from
20 mg tissue
3-5 µg from 100 mg Arabidopsis leaf (Mini)
37 µg from 1x109
E. coli
10 µg from 2x108 S. cerevisiae
cells
300 ng from 6 mm
blood spots
2 µg per swab
10-40 µg from
20 mg tissue
 
 
80 µg from 500 mg Lemon leaf (Maxi)
10-15 µg from 1x109
Bacillus subtilis
   
Elution Volume30-100 µl30-200 µl30-200 µl

30-200 µl (Mini)

1 ml (Maxi)

30-200 µl30-200 µl30-100 µl50-100 µl
Operation Time<20 minutes<25 minutes30 minutes<30 minutes<30 minutes<40 minutes<20 minutes<20 minutes
Related Genomic DNA Extraction Products
Product Name
RXNS
Catalogue Number
100, 300 
GS100/300
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GB100/300
10, 25
GPM010/25
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GDM10/25
50, 100, 300
GT050/100/300
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GP100
100
GMB100/300
100
GSK100/300
100, 300
GBYB100/300
100, 300
GBB100/101/300/301
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96GBP04/10
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