Magnetic Beads Genomic DNA Extraction Kit (Bacteria) ME048/ME096

Magnetic Beads Genomic DNA Extraction Kit Bacteria
Magnetic Beads Genomic DNA Extraction Kit (Bacteria) ME048/ME096

The Magnetic Beads Genomic DNA Extraction Kit Bacteria was designed specifically for efficient genomic DNA purification from Gram (-) negative and Gram (+) positve bacteria. DNA is bound to the surface of the magnetic beads and released using a proprietary buffer system. The kit can be easily adapted to automated magnetic bead separation instruments and workstations. The purified DNA can be used in qPCR and a variety of other downstream applications.
 

Advantages (Cat. # ME048, ME096)
  • High Yield: >26 µg of Genomic DNA
  • High Quality DNA: A260/A280 = 1.8-2.0
  • Magnetic Bead Concentration: 50 mg/ml
  • Magnetic Bead Size: ~ 5 µm
  • Easily adapted to automated magnetic bead separation instruments and workstations
  • Sample: 2 x 109 of bacteria cells (Gram + and Gram -)
  • Manual or automated DNA isolation
  • Operation time: within 30 minutes (manual) 
  • Storage: dry at room temperature (15-25ºC) for up to 1 year without showing any reduction in performance, Lysozyme is shipped at room temperature and should be stored at -20°C for up to 1 year
Applications
 
qPCR, PCR, Real-time PCR, Automated Fluorescent DNA Sequencing, Southern Blotting, Next Generation Sequencing (NGS)
 
Components
  • ME1 Buffer
  • ME2 Buffer
  • ME3 Buffer
  • ME4 Buffer
  • Lysozyme
  • Proteinase K
  • MW1 Buffer
  • MW2 Buffer
  • ME Magnetic Beads
  • Elution Buffer
Quality Control
 
The quality of the The Magnetic Beads Genomic DNA Extraction Kit Bacteria is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system by isolating genomic DNA from 1 x 109 cells of Escherichia coli and from  1 x 109 cells of Bacillus subtilis. 5 μl of extracted DNA product from a 100 μl eluate is analyzed on a 0.8% agarose gel.
 
Magnetic Beads Genomic DNA Extraction Kit (Bacteria) Functional Test Data 

Figure 1. Genomic DNA from 1 x 109 cells of Escherichia coli and from  1 x 109 cells of Bacillus subtilis was extracted in triplicate using the Magnetic Beads Genomic DNA Extraction Kit Bacteria. 5 μl aliquots of extracted DNA product from a 100 μl eluate were analyzed on a 0.8% agarose gel.
Lane 1-3: Escherichia coli
M = Geneaid 1 Kb DNA Ladder
Lane 4-6: Bacillus subtilis
Test
Sample Bacteria Strain
260/280
260/230
Yield
1
Escherichia coli
2.06
1.95
24.48 µg
2
Escherichia coli
2.06
1.90
22.49 µg
3
Escherichia coli
2.07
2.02
26.47 µg
4
Bacillus subtilis
2.06
2.28
26.27 µg
5
Bacillus subtilis
2.06
2.24
22.20 µg
6
Bacillus subtilis
2.05
2.25
26.46 µg
Table 1. Yield of Genomic DNA from 1 x 109 cells of Escherichia coli and from  1 x 109 cells of Bacillus subtilis extracted in triplicate using the Magnetic Beads Genomic DNA Extraction Kit Bacteria.
 

Figure 2. Extracted DNA products were used as a DNA template for amplifying 16S ribosomal RNA gene. 5 μl aliquots of PCR product were loaded in each well. A 1.5 kb 16S ribosomal gene fragment was successfully amplified from each DNA product.
Lane 1-3: Escherichia coli
M = Geneaid 1 Kb DNA Ladder
Lane 4-6: Bacillus subtilis
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