Magnetic Beads Genomic DNA Extraction Kit (Plant) MP048/MP096

Magnetic Beads Genomic DNA Extraction Kit Plant
Magnetic Beads Genomic DNA Extraction Kit (Plant) MP048/MP096

The Magnetic Beads Genomic DNA Extraction Kit Plant was designed specifically for efficient genomic DNA purification from a variety of plant species. DNA is bound to the surface of the magnetic beads and released using a proprietary buffer system. The Magnetic Beads Genomic DNA Extraction Kit Plant can be easily adapted to automated magnetic bead separation instruments and workstations. The purified DNA can be used in qPCR and other downstream applications.

Advantages (Cat. # MP048, MP096)
  • High Yield: 1-20 µg of Genomic DNA
  • High Quality DNA: A260/A280 = 1.8-1.9
  • Magnetic Bead Concentration: 50 mg/ml
  • Magnetic Bead Size: ~ 5 µm
  • Easily adapted to automated magnetic bead separation instruments and workstations
  • Sample: 10-50 mg of fresh or frozen plant tissue
  • Manual or automated DNA isolation
  • Operation time: within 30 minutes (manual) 
  • Storage: dry at room temperature (15-25ºC)
Applications
 
qPCR, PCR, Real-time PCR, Automated Fluorescent DNA Sequencing, Southern Blotting, Next Generation Sequencing (NGS)
 
Components
  • MP1 Buffer
  • MP2 Buffer
  • RNase A
  • MW1 Buffer
  • MW2 Buffer
  • MP Magnetic Beads
  • Elution Buffer
Quality Control
 
The quality of the The Magnetic Beads Genomic DNA Extraction Kit Plant is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system by isolating genomic DNA from a 50 mg Arabidopsis sample.
 
Magnetic Beads Genomic DNA Extraction Kit (Plant) Functional Test Data 
Figure 1. Genomic DNA was extracted using the Magnetic Beads Genomic DNA Extraction Kit Plant. 5 µl of purified genomic DNA from 2 different plant leaves was analyzed on a 0.8% agarose gel.
M = Geneaid 1 Kb DNA Ladder
Lane 1-3 = Benthamiana nicotiana leaf
Lane 4-6 = Tomato leaf
 
Plant Species
Sample
DNA Conc.
260/280
Yield
Benthamiana nicotiana
50 mg leaf
30 ng/µl
1.7-1.8
3 µg
Tomato
50 mg leaf
65 ng/µl
1.7-1.8
6.5 µg
Table 2. Yield of purified DNA from 50 mg fresh leaf samples.

Figure 2. Genomic DNA was extracted using the Magnetic Beads Genomic DNA Extraction Kit Plant. 2 µl of purified genomic DNA from 3 different plant leaves was analyzed on a 0.8% agarose gel.
M = Geneaid 1 Kb DNA Ladder
Lane 1-2 = Arabidopsis leaf
Lane 3-4 = lemon leaf
Lane 5-6 = coffee leaf
Plant Species
Sample
DNA Conc.
260/280
Yield
Arabidopsis
50 mg leaf
20 ng/µl
1.8-1.9
2 µg
Lemon
50 mg leaf
150 ng/µl
1.8-1.9
15 µg
Coffee
50 mg leaf
90 ng/µl
1.7-1.8
9 µg
Table 2. Yield of purified DNA from 50 mg fresh leaf samples.
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