Magnetic Beads Soil Genomic DNA Extraction Kit MS048/MS096

Soil DNA Extraction Kit
Magnetic Beads Soil Genomic DNA Extraction Kit MS048/MS096

The Magnetic Beads Soil DNA Extraction Kit was designed specifically for rapid isolation of genomic DNA from microorganisms such as bacteria, archaea, fungi, and algae in soil samples. The soil sample is homogenized using a lysis buffer combined with ceramic beads. Insoluble particles, proteins and PCR inhibitors such as humic acid are then precipitated using a unique inhibitor removal buffer. Residual PCR inhibitors are further removed by passing through a specialized PCR inhibitor removal column. DNA is bound to the surface of the magnetic beads and released using a proprietary buffer system. The Magnetic Beads Soil Genomic DNA Extraction Kit can be easily adapted to automated magnetic bead separation instruments and workstations. The purified DNA can be used in qPCR and a variety of other downstream applications.

Advantages (Cat. # MS048, MS096)
  • High Quality DNA: up to 5 µg of Genomic DNA, A260/A280 = 1.8-2.0
  • Sample: 250-400 mg soil
  • Easily adapted to automated magnetic bead separation instruments and workstations
  • Magnetic Bead Size: ~ 5 µm
  • Manual or automated DNA isolation
  • Operation time: within 40 minutes (manual) 
  • Storage: dry at room temperature (15-25ºC)
Applications
 
Fluorescent and Radioactive Sequencing, PCR, Restriction Enzyme Digestion, DNA Labeling and Ligation, Microarray, Transfection
 
Components
  • MS1, MS2, MS3 Buffer
  • BeadBeating Tubes
  • 2 ml Collection Tubes
  • Inhibitor Removal Columns
  • MW1 Buffer
  • MW2 Buffer
  • MS Magnetic Beads
  • Elution Buffer
Quality Control
 
The quality of the The Magnetic Beads gDNA Kit (Soil) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. DNA is purified from 250 mg soil samples and analyzed by electrophoresis.