Magnetic Beads PCR Cleanup Kit MC048/MC096

Magnetic Beads PCR Cleanup Kit
Magnetic Beads PCR Cleanup Kit MC048/MC096

The Magnetic Beads PCR Cleanup Kit was designed to recover or concentrate DNA fragments from PCR or other enzymatic reactions. DNA is bound to the surface of the magnetic beads and released using a proprietary buffer system. The Magnetic Beads PCR Cleanup Kit can be easily adapted to automated magnetic bead separation instruments and workstations. The purified DNA can be used in a variety of downstream applications.

Advantages (Cat. # MC048, MC096)
  • High Yield: >70% recovery
  • High Quality DNA: A260/A280 = 1.8-2.0
  • Magnetic Bead Concentration: 50 mg/ml
  • Magnetic Bead Size: ~ 5 µm
  • Broad Fragment Size Range: 100 bp–10 kb
  • Easily adapted to automated magnetic bead separation instruments and workstations
  • Sample: up to 100 µl of PCR products
  • Manual or automated DNA isolation
  • Operation time: within 20 minutes (manual) 
  • Storage: dry at room temperature (15-25ºC) for up to 1 year without showing any reduction in performance
Applications
 
Fluorescent and Radioactive Sequencing, PCR, Restriction Enzyme Digestion, DNA Labeling and Ligation, Microarray, Transfection
 
Components
  • MC Buffer
  • MW1 Buffer
  • MW2 Buffer
  • MC Magnetic Beads
  • Elution Buffer
Quality Control
 
The quality of the The Magnetic Beads PCR Cleanup Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. DNA fragments are purified from PCR products and analyzed by electrophoresis.
 
Magnetic Beads PCR Cleanup Kit Functional Test Data 

Figure 1. PCR product DNA fragments ranging from 250 bp-10 kb were extracted using the Magnetic Beads PCR Cleanup Kit (lane 1, 2, 3). The purified DNA from a 50 µl eluate was confirmed by spectrophotometer and analyzed by electrophoresis on a 1% agarose gel. M = Geneaid 1 Kb DNA Ladder (control, total DNA = 3 µg)
Test
DNA Conc.
260/280
Yield
Recovery
1
43.0 ng/µl
1.81
2.15
71.7%
2
42.2 ng/µl
1.81
2.11
70.3%
3
42.6 ng/µl
1.82
2.13
71.0%
Table 1. Recovery of purified DNA fragments from 100 µl of PCR product.
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