The Magnetic Beads Virus DNA/RNA Extraction Kit was designed specifically for efficient purification of viral DNA and viral RNA from cell-free samples such as serum, plasma, body fluids and the supernatant of viral infected cell cultures. Viral DNA/RNA is bound to the surface of the magnetic beads and released using a proprietary buffer system. The Magnetic Beads Viral DNA/RNA Kit can be easily adapted to automated magnetic bead separation instruments and workstations. The purified DNA/RNA can be used in qPCR and qRT-PCR assays.
Figure 1. Virus RNA was purified from 10E1-10E4 copy number of Red Spotted Grouper Nervous Necrosis Virus (RGNNV) using the Geneaid Magnetic Beads Virus DNA/RNA Extraction Kit (2 replications of each copy number). The purified RNA was eluted with 50 μl RNase-free Water. cDNA synthesis was carried out with a 10 μl aliquot of purified RNA using a Transcriptor First Strand cDNA Synthesis Kit (Roche) in a final volume of 20 μl. A Real-time PCR assay was then performed with 5 μl of synthesized cDNA as template, primers (designed to amplify the T4 region on the RNA2 segment), and Fast SYBR Green PCR Master Mix using the StepOnePlusTM Real-Time PCR system (Applied Biosystems). The results confirmed that virus RNA can be successfully extracted and detected from as low as 10E1 copy number of RGNNV.
|Product Name||RXNS||Catalogue Number|
|Magnetic Beads Genomic DNA Extraction Kit (Blood)||48, 96||MB048/96|
|Magnetic Beads Viral DNA/RNA Extraction Kit||48, 96||MV048/96|
|Magnetic Beads Genomic DNA Extraction Kit (Bacteria)||48, 96||ME048/96|
|Magnetic Beads PCR Cleanup Kit||48, 96||MC048/96|
|Magnetic Beads Micro gDNA Extraction Kit||48, 96||MM048/96|
|Magnetic Beads Genomic DNA Extraction Kit (Plant)||48, 96||MP048/96|