High Fidelity (HiFi) Taq DNA Polymerase is an enzyme blend of recombinant Pfu and recombinant Taq DNA Polymerase with an efficient proofreading 3’ – 5’ exonuclease activity for improved PCR reactions. High Fidelity Taq DNA Polymerase is an efficient enzyme mixture that greatly increases fidelity and amplification of genomic targets up to 10 kb with high specificity, sensitivity, accuracy, and yield.
Advantages (Cat.# HT050)
- Proofreading 3’ – 5’ exonuclease activity
- High Fidelity
- Recombinant sourced Taq DNA Polymerase
- Format: 500 U
- Amplification: genomic targets up to 10 kb
- Storage: -20ºC for extended periods
- Shipping: 2-8ºC (dry ice is not required)
- 3' – 5' Exonuclease Proofreading Ability: YES
- 5' – 3' Exonuclease Activity: YES
Full-length Gene Amplification, Gene Mutation Detection, L-PCR, PCR-ELISA
HiFi Taq DNA Polymerase is expressed and purified from an E. coli strain which carries the Taq DNA Polymerase gene from Thermus aquaticus.
Storage Buffer: 20 mM Tris-HCl, pH8.8, 0.1 mM EDTA, 1 mM DTT, 1.0% Triton X-100, 50% Glycerol.
10X PCR Buffer: 100 mM KCl, 20 mM MgSO4•7H2O, 200 mM Tris-HCl, pH8.8, 1.0% Triton X-100, 100 mM (NH4)2SO4, 1 mg/ml BSA. KCl and (NH4)2SO4 are included in 10X PCR Buffer in order to both increase annealing temperature range and increase varying Mg2+ concentration tolerance.
One unit catalyzes the incorporation of 10 nmol of dNTP into acid insoluble form in 30 minutes at 70ºC.
The quality of HiFi Taq DNA Polymerase is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. PCR is used to ensure consistent amplification of genomic targets using HiFi Taq DNA Polymerase.