Taq DNA Polymerase

Taq DNA Polymerase 10X PCR Buffer premixed with MgCl2
Taq DNA Polymerase

Taq DNA Polymerase (10X PCR Buffer is premixed with MgCl2is a thermostable enzyme which catalyzes the polymerization of nucleotides in the 5' to 3' direction at 70-74°C and exhibits a half life of 30 minutes at 95°C. This enzyme has a 5'-3' DNA polymerase activity and a 5'-3' exonuclease activity. The amplified products are up to 8 kb with 3’ adenosine residues and are ready to use directly in TA cloning.

Advantages (Cat. # TQ050, TQ250, TQN050, TQN250) 
  • 10X PCR Buffer is premixed with MgCl2 (20 mM)
  • Recombinant sourced Taq DNA Polymerase
  • Amplification: up to 8 kb
  • Storage: -20ºC for extended periods
  • Shipping: 2-8ºC (dry ice is not required)
  • 3' to 5' Exonuclease Poofreading Abiliy: NO
  • 5' to 3' Exonuclease Activity: YES
Applications
 
PCR, Screening, Primer Extension, Amplification, Terminal dA Tailing
 
Source

Taq DNA Polymerase is expressed and purified from an E. coli strain which carries the Taq DNA Polymerase gene from Thermus aquaticus.

Composition

Storage Buffer: 20 mM Tris-HCl, pH8.0, 0.1 mM EDTA, 1 mM DTT, 1.0% Triton X-100, 50% Glycerol

10X PCR Buffer: 150 mM Tris-HCl, pH8.75 at 25ºC, 500 mM KCl, 20 mM MgCl2, 1.0% Triton X-100

dNTPs (10 mM each): provided with TQN050 and TQN250

Unit Definition

One unit incorporates 10 nmol of dNTP into acid insoluble material in 30 minutes at 74ºC.

Quality Control

The quality of Taq DNA Polymerase is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Quality control assays include activity test, PCR, endonuclease activity, exonuclease activity.