Advantages (Cat. # TQ052, TQ252, TQN052, TQN252)
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10X PCR Buffer premixed with MgSO4
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Recombinant sourced Taq DNA Polymerase
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Format: 500 U, 2500 U
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Amplification: up to 8 kb
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Storage: -20ºC for extended periods
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Shipping: 2-8ºC (dry ice is not required)
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3' to 5' Exonuclease Poofreading Abiliy: NO
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5' to 3' Exonuclease Activity: YES
Applications
PCR, Screening, Primer Extension, Amplification, Terminal dA Tailing
Source
Taq DNA Polymerase is expressed and purified from an E. coli strain which carries the Taq DNA Polymerase gene from Thermus aquaticus.
Composition
Storage Buffer: 20 mM Tris-HCl pH8.0, 0.1 mM EDTA, 1 mM DTT, 1.0% Triton X-100, 50% Glycerol
10X PCR Buffer: 200 mM Tris-HCl pH8.8 at 25ºC, 100 mM KCl, 100 mM (NH4)2SO4, 20 mM MgSO4, 1.0% Triton X-100
dNTPs (10 mM each): provided with TQN052 and TQN252
Unit Definition
One unit incorporates 10 nmol of dNTP into acid insoluble material in 30 minutes at 74ºC.
Quality Control
The quality of Taq DNA Polymerase is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Quality control assays include activity test, PCR, endonuclease activity, exonuclease activity.