Taq DNA Polymerase 1.2

Taq DNA Polymerase 10X PCR Buffer premixed with MgSO4

Taq DNA Polymerase 1.2 (10X PCR Buffer is premixed with MgSO₄) is a thermostable enzyme which catalyzes the polymerization of nucleotides in the 5' to 3' direction at 70-74°C and exhibits a half life of 30 minutes at 95°C . This enzyme has a 5'-3' DNA polymerase activity and a 5'-3' exonuclease activity. The amplified products are up to 8 kb with 3’ adenosine residues and are ready to use directly in TA cloning.

Advantages (Cat. # TQ052, TQ252, TQN052, TQN252)

10X PCR Buffer premixed with MgSO₄
Recombinant sourced Taq DNA Polymerase
Format: 500 U, 2500 U
Amplification: up to 8 kb
Storage: -20ºC for extended periods
Shipping: 2-8ºC (dry ice is not required)
3' to 5' Exonuclease Poofreading Abiliy: NO
5' to 3' Exonuclease Activity: YES

Applications

PCR, Screening, Primer Extension, Amplification, Terminal dA Tailing

Source

Taq DNA Polymerase is expressed and purified from an E. coli strain which carries the Taq DNA Polymerase gene from Thermus aquaticus.

Composition

Storage Buffer: 20 mM Tris-HCl pH8.0, 0.1 mM EDTA, 1 mM DTT, 1.0% Triton X-100, 50% Glycerol

10X PCR Buffer: 200 mM Tris-HCl pH8.8 at 25ºC, 100 mM KCl, 100 mM (NH₄)₂SO₄, 20 mM MgSO₄, 1.0% Triton X-100

dNTPs (10 mM each): provided with TQN052 and TQN252

Unit Definition

One unit incorporates 10 nmol of dNTP into acid insoluble material in 30 minutes at 74ºC.

Quality Control

The quality of Taq DNA Polymerase is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Quality control assays include activity test, PCR, endonuclease activity, exonuclease activity.