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Ultra-Pure Taq DNA Polymerase 1.2

Taq DNA Polymerase 10X PCR Buffer premixed with MgSO4
Ultra-Pure Taq DNA Polymerase 1.2

Ultra-Pure Taq DNA Polymerase 1.2 (10X PCR Buffer premixed with MgSO4) is a thermostable enzyme which is purified to reduce levels of contaminating DNA, making it well suited for PCR and sensitive experiments using bacterial templates or random primers. The high purity of this Taq DNA Polymerase makes it ideal in detecting and identifying bacterial DNA, and is a more accurate method for mutation scanning techniques while preventing the amplification of undesired DNA sequences. Ultra-Pure Taq DNA Polymerase is suitable for work in bacterial genomics due to the reduced probability of contamination leading to non-specific amplification or artifacts during PCR reactions. The amplified products are up to 8 kb with 3’ adenosine residues and are ready to use directly in TA cloning.

Advantages (Cat. # UT052, UT252, UTN052, UTN252) 
  • 10X PCR Buffer premixed with MgSO4
  • Recombinant sourced Taq DNA Polymerase
  • Excellent for PCR and other sensitive experiments
  • Accurate method for mutation scanning techniques while preventing the amplification of undesired DNA sequences
  • Ideal in detecting and identifying bacterial DNA
  • Minimized levels of DNA contamination
  • Format: 500 U, 2500 U 
  • Amplification: up to 8 kb
  • Storage: -20ºC for extended periods
  • Shipping: 2-8ºC (dry ice is not required)
  • 3' to 5' Exonuclease Poofreading Abiliy: NO
  • 5' to 3' Exonuclease Activity: YES
Applications
 
PCR, Screening, Primer Extension, Amplification, Terminal dA Tailing
 
Source

Ultra-Pure Taq DNA Polymerase is expressed and purified from an E. coli strain which carries the Taq DNA Polymerase gene from Thermus aquaticus.

Composition

Storage Buffer: 20 mM Tris-HCl pH8.0, 0.1 mM EDTA, 1 mM DTT, 1.0% Triton X-100, 50% Glycerol

10X PCR Buffer: 200 mM Tris-HCl pH8.8 at 25ºC, 100 mM KCl, 100 mM (NH4)2SO4, 20 mM MgSO4, 1.0% Triton X-100

dNTPs (10 mM each): provided with UTN052 and UTN252

Unit Definition

One unit incorporates 10 nmol of dNTP into acid insoluble material in 30 minutes at 74ºC.

Quality Control

The quality of Ultra-Pure Taq DNA Polymerase is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Quality control assays include activity test, PCR, endonuclease activity, exonuclease activity.
 
Ultra-Pure Taq DNA Polymerase Functional Test

Figure 1. Following 30 PCR cycles a 950 bp bacterial 16S ribosomal DNA fragment was successfully amplified in the lane 1 PCR reaction using Ultra-Pure Taq DNA Polymerse + bacterial genomic DNA template (5 µl of DNA product was loaded into a 1% agarose gel and checked by electrophoresis).

Lane M: Geneaid 1 Kb DNA Ladder
Lane 1: Ultra-Pure Taq DNA Polymerase + Bacterial gDNA Template
Lane 2: Conventional Taq DNA Polymerase (no bacterial gDNA template)
Lane 3: Ultra-Pure Taq DNA Polymerase (no bacterial gDNA template)

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