Maxi Plasmid DNA Kit (PM010, PM025)

Maxi Plasmid Kit
Maxi Plasmid DNA Kit (PM010, PM025)

The Geneaid™ Maxiprep Plasmid Kit was designed for plasmid DNA purification using pre-packed anion-exchange resin maxiprep plasmid columns to purify plasmid DNA from 200-1600 ml of cultured bacterial cells. TrueBlue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. This plasmid kit uses a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. Using an efficient gravity-flow procedure, plasmid DNA in the crude lysate is bound to the plasmid maxi column and the contaminants are removed with PW Buffer. The purified plasmid DNA is eluted by a high salt buffer and then precipitated with Isopropanol for desalting. The entire procedure can be completed in 2.5 hours without ultracentrifuges, HPLC or other toxic reagents and the purified plasmid DNA is suitable for Transfection, Sequencing Reactions, Ligation, PCR, and In-vitro Transcription, Microinjection, Restriction Enzyme Digestion and Gene Gun.

 

 

 

Advantages (Cat. # PM010, PM025)

  • High Yield: 1.5 mg of pure transfection grade plasmid DNA from 400 ml cultured bacterial cells
  • Large Sample Volume: 200-1600 ml of cultured bacterial cells
  • Format: plasmid maxi anion-exchange resin columns
  • Elution Volume: 500 µl - 2 ml
  • Operation time: within 2.5 hours
  • Storage: dry at room temperature (15-25ºC)

Applications

Transfection, Sequencing, Ligation, PCR, In-vitro Transcription Microinjection, Restriction Enzyme Digestion, Gene Gun

Compnents

  • PM1, PM2, PM3 Buffer
  • TrueBlue Lysis Buffer
  • PEQ Buffer
  • PW Buffer
  • PEL Buffer
  • RNase A
  • Plasmid Maxi Columns

Quality Control

The quality of the Geneaid™ Plasmid Maxi Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Plasmid DNA from a 400 ml overnight E. coli (DH5α) culture, containing plasmid pBluescript (A600 > 2 U/ml) is purified using the Geneaid™ Maxi Plasmid Kit. Following the purification process, a yield of more than 900 μg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.

Plasmid Maxi Kit Funtional Test Data

Figure 1. Plasmid DNA was extracted using the Geneaid™ Maxi Maxi Kit. The purified supercoiled plasmid DNA [400 ml overnight E. coli (DH5α) culture, containing a 3 kb plasmid pBluescript (A600 > 2 U/ml, OD600 = 4.0)], was used in EcoRI digestion and analyzed by electrophoresis on a 1% agarose gel. M = Geneaid 1 Kb DNA Ladder
Sample
DNA Conc.
260/280
260/230
Total Yield
Test 1 1550.10 µg/ml 1.87 2.25 1.55 mg
Test 2 1540.10 µg/ml 1.87 2.29 1.54 mg 
Plasmid DNA Midi and Maxi Kits Specifications
Specifications
Cat. # PIF025 PIFE25 PI025 PIE25 PM010/25 PME10/25
Format
Anion-Exchange
Gravity Flow
Anion-
Exchange
Gravity Flow
Anion-Exchange
Gravity Flow
Anion-
Exchange
Gravity Flow
Anion-Exchange
Gravity Flow
Anion-
Exchange
Gravity Flow
Binding Capacity 500 µg 500 µg 800 µg 800 µg 1.5 mg 1.5 mg
Filter Column Yes Yes N/A N/A N/A N/A
Endotoxin Level <0.15 EU/µg <0.10 EU/µg <0.15 EU/µg <0.10 EU/µg <0.15 EU/µg <0.10 EU/µg
Culture Input 50-150 ml 50-150 ml 50-300 ml 50-300 ml 200-1600 ml 200-1600 ml
Plasmid Size 1-20 kb 1-20 kb 1-20 kb 1-20 kb 1-20 kb 1-20 kb
Typical Yield 200-500 µg 200-500 µg 200-800 µg 200-800 µg 1-1.5 mg 1-1.5 mg
Elution Volume 500 µl-2 ml 500 µl-2 ml 500 µl-2 ml 500 µl-2 ml 500 µl-2 ml 500 µl-2 ml
Operation Time <80 minutes <80 minutes <120 minutes <120 minutes <180 minutes <180 minutes
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