The Prestained Protein Ladder (245 kDa) is a three-color prestained protein standard which includes protein dye for convenient protein sample staining. The protein ladder consists of 12 prestained proteins covering a wide range of molecular weights (10 to 245 kDa). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The Prestained Protein Ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. The ladder is supplied in a gel loading buffer and is ready to use, without heating, diluting, or a reducing agent prior to loading.
Advantages (Cat. # PL0245)
Monitor protein migration/sample in SDS-PAGE, Monitor protein transfer onto membranes during Western Blotting, Sizing of proteins on SDS-PAGE and Western blots
The quality of the Prestained Protein Ladder (245 kDa) is tested on a lot-to-lot basis according to Geneaid's ISO certified quality management system. 5 μl of Prestained Protein Ladder (245 kDa) resolves 12 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to nitrocelluslose membrane.
Prestained Protein Ladder V Guide for Molecular Weight Estimation (kDa)
Figure 1. Migration patterns of various electrophoretic conditions (according to type and percentage of running buffer). The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoretic condition.
|Product Name||Volume||Catalogue Number|
Prestained Protein Ladder (245 kDa)
|2 M x 500 µl||
|2 ml, 5X||