Prestained Protein Ladder V

Prestained Protein Ladder 10 - 180 kDa
Prestained Protein Ladder V

The Prestained Protein Ladder V is a three-color prestained protein standard which includes protein dye for convenient protein sample staining. The protein ladder consists of 10 prestained proteins covering a wide range of molecular weights (10 to 180 kDa). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band) when separated on SDS-PAGE. The Ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. The ladder is supplied in a gel loading buffer and is ready to use, without heating, diluting, or a reducing agent prior to loading. 

Advantages (Cat. # PL005)
  • Wide Molecular Weight range: 10-180 kDa
  • Supplied buffer for direct gel loading
  • Sharp bands with 28 and 75 KDa reference bands (green/red dye)
  • Includes Protein Loading Dye (2 ml, 5X)
  • 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively
  • 2~3 μl per well for general Western transferring
  • Increase application amount for thicker or larger gel
  • Optional Dithiothreitol (DTT) procedure

Storage

  • 25ºC for 2 weeks
  • 4ºC for 3 months
  • -20ºC for 24 months

Applications

Monitor protein migration/sample in SDS-PAGE, Monitor protein transfer onto membranes during Western Blotting, Sizing of proteins on SDS-PAGE and Western blots

Components

  • Prestained Protein Ladder V (approximately 0.2~0.4 mg/ml of each protein in buffer {20 mM Trisphosphate pH7.5 at 25°C, 2% SDS, 1 mM 2-Mercaptoethanol, 3.6 M Urea and 15% (v/v) glycerol}.
  • Protein Loading Dye (2 ml, 5X)

Quality Control

The quality of the Prestained Protein Ladder V is tested on a lot-to-lot basis according to Geneaid's ISO certified quality management system. 5 μl of Prestained Protein Ladder V resolves 10 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane.

Prestained Protein Ladder V Guide for Molecular Weight Estimation (kDa)

 

 

 

Figure 1. Migration patterns of various electrophoretic conditions (according to type and percentage of running buffer). The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoretic condition.

 

 

 

 

Figure 2. Prestained Protein Ladder V functional tests of 1 µl - 10 µl loading volumes from left to right respectively.
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