Reverse Protein Stain Kit

Reverse Protein Stain Kit
Reverse Protein Stain Kit

The Reverse Protein Stain Kit uses imidazole and zinc salts for protein detection as low as 1 ng in electrophoresis gels. The method is based on selective precipitation of a white imidazole–zinc complex in the gel, except in those zones where proteins are located. These zones remain transparent. When the gel is placed on a dark background, the negative gel image can be converted to a positive image with black bands, spots, and a white background. This stain is as sensitive as most silver stains and requires only 10 minutes to complete. In addition, fixation of proteins to the gel is not needed, there is no interaction of the stain with the protein, and complete destaining of the matrix can be achieved. Imidazole-zinc reverse stain uses only two staining solutions, which can be easily diluted from stock solutions. For the imidazole-zinc negative staining, no fixation is necessary. Gels are typically stained immediately after electrophoresis. Imidazole-zinc reverse stain is fully compatible with down-stream applications, such as mass spectrometry, Edman sequencing, electroelution, and membrane blotting techniques.

Advantages (Cat. # PS500, PS505)
  • Protein detection as low as 1 ng in electrophoresis gels
  • Fixation of proteins to the gel is not needed so will not affect protein samples
  • Complete destaining of the matrix can be achieved
  • Rapid Staining: < 10 minutes compared to conventional staining methods requiring up to 24 hours
  • Non toxic (no organic solvents)
  • Environmentally friendly
  • Alternative staining methods can be used direclty following this procedure
  • Storage: room temperature for up to 2 years
Applications
 
Imidazole-zinc reverse stain is fully compatible to down-stream applications, such as Mass spectrometry, Edman sequencing, Electroelution, and Membrane blotting techniques
 
Components
  • Protein Stain Buffer I
  • Protein Stain Buffer II
  • Black Stain Box

Quality Control

The quality of the Reverse Protein Stain Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system.

Figure 1. The Reverse Protein Stain Kit uses imidazole and zinc salts for protein detection as low as 1 ng in electrophoresis gels. The method is based on selective precipitation of a white imidazole–zinc complex in the gel, except in those zones where proteins are located.
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Reverse Protein Stain Kit
 500 ml
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