ZEROprep™ DNA Reagent (ZDR050, ZDR100)

DNA For Direct PCR Without DNA Purification
ZEROprep™ DNA Reagent (ZDR050, ZDR100)
ZEROprep™ DNA Reagent is designed for efficient release of DNA for direct use in PCR reactions without purification. A wide variety of samples are effectivly homogenized in the reagent without any pre-treatment or subsequent bind, wash or elution steps. Simply place the sample in the reagent, follow the 2 step protocol and transfer the lysate to a PCR mix.

 

 

Advantages (Cat. # ZDR050, ZDR100)
  • Use DNA directly in PCR reactions
  • DNA purification is not required
  • 15 minute, 2 step protocol
  • Process a wide variety of sample types (tissue, blood, plant, bacteria, yeast/fungus, virus etc.)
  • Storage: dry at room temperature (15-25ºC)
Applications
 
PCR, Multiplex PCR, Real-time PCR
 
Components
  • ZEROprep™ DNA Reagent 
Quality Control
 
ZEROprep™ DNA Reagent is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. DNA from a 1 mg tissue sample is lysed in ZEROprep™ DNA Reagent. A 5 µl aliquot of lysate is added directly into a 50 µl PCR mix.
 
ZEROprep™ DNA Reagent PCR Data
Figure 1. Human blood samples were lysed in ZEROprep™ DNA reagent for 15 minutes at room temperature. Plant and bacteria samples were lysed for 15 minutes at 80ºC. 2 μl aliquots of lysate were added to 20 μl PCR mixtures. Lane 1-2: partial human ACTB gene (250 bp) was amplified from 10 μl of human blood. Lane 3-4: partial Actin-2 gene (750 bp) was amplified from 20 mg of Arabidopsis thaliana leaves. Lane 5: partial 16S ribosomal gene (200 bp) was amplified from 2 μl of cultured Escherichia coli broth. Lane 6: partial 16S ribosomal gene (200 bp) was amplified from 2 μl of cultured Bacillus subtilis broth.

Reagent Genomic DNA Extraction Kits Specifications

Specifications
Cat. #
GEB100
GEB01K
GEB01K+
GEC150
GEC1.5K
GEC1.5K+
GET150
GET1.5K
GET1.5K+
GEE150
GEE1.5K
GEE1.5K+
GBY100
GBY300
GR100
GR200
RXNS 100-1,000 150-1,500 150-1,500 150-1,500 100-300 100-200
Format
Reagent System
Reagent System
Reagent System
Reagent System
Reagent System
Reagent System
Sample
300 µl-10 ml blood
up to 5x107 cultured cells
up to 200 mg tissue
up to 1.5x109 bacterial cells
up to 2x108 yeast cells
up to 1 g plant tissue
Elution Volume
100 µl-1 ml
50-200 µl
100-200 µl
100 µl
50-100 µl
50-100 µl
Operation Time
<40 minutes
<60 minutes
<70 minutes
<60 minutes
<40 minutes
<50 minutes
Related Genomic DNA Extraction Products
Product Name RXNS Catalogue Number
150/1,500
GEE100/01K/01K+
100/1,000
GEB100/01K/01K+
150/1,500
GET150/1.5K/1.5K+
150/1,500
GEC150/1.5K/1.5K+
Geneaid™ DNA Isolation Kit (Yeast) 100, 300 GEY100/300
GENEzol™ DNA Reagent Plant 100, 200 GR100/200