Yeast DNA Isolation Kit (GEY100, GEY300)

DNA Isolation Yeast Kit
Yeast DNA Isolation Kit (GEY100, GEY300)

The Geneaid™ DNA Isolation Kit (Yeast) offers a simple and gentle reagent DNA precipitation method for isolating high molecular weight genomic, mitochondrial or viral DNA from Saccharomyces cerevisiae and a variety of other yeast and fungus species. This highly versatile solution based system offers a convenient procedure with minimal hands on time. The provided Sorbitol Buffer, when combined with zymolase or lyticase, will efficiently lyse yeast and other fungus species cell walls consisting of chitin and polysaccharides. Purified DNA (A260/A280 = 1.8-2.0), is suitable for use in PCR or other enzymatic reactions.

Advantages (Cat. # GEY100, GEY300)
  • Purify genomic DNA from (up to 2 x 108) yeast and other fungus species using a simple and gentle 3 buffer system
  • Simple and gentle reagent DNA precipitation method
  • Convenient premixed Sorbitol Buffer!
  • Time: 40 minutes
  • Elution volume: 50-100 µl 
  • Storage: dry at room temperature (15-25°C)

Applications

DNA archiving, PCR, AFLP, RFLP/PADP, Southern Blotting, Real-time PCR

Components

  • Sorbitol Buffer 
  • Cell Lysis Buffer
  • Protein Removal Buffer
  • DNA Hydration Buffer

Quality Control

The quality of the Presto™ Mini gDNA Yeast Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. The purified genomic DNA (A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.

Geneaid™ DNA Isolation Kit (Yeast) Funtional Test Data

Figure 1. Genomic DNA (approximately 30 kb) was extracted using the Geneaid™ DNA Isolation Kit (Yeast). Saccharomyces cerevisiae (2×108) was harvested by centrifugation at 5,000 x g for 10 minutes. A 15 µl aliquot of purified genomic DNA from a 100 µl eluate was analyzed by electrophoresis on a 1% agarose gel. M = Geneaid 1 Kb DNA Ladder

Test DNA Conc. 260/280 260/230 Yield
1 115.5 µg/ml 1.91 1.87 11.6 µg
2 142.9 µg/ml 1.92 2.01 14.3 µg
3 137.1 µg/ml 1.91 1.97 13.7 µg
Reagent Genomic DNA Extraction Kits Specifications
Specifications
Geneaid™ DNA Isolation Kit Yeast
Cat. #
GEB100
GEB01K
GEB01K+
GEC150
GEC1.5K
GEC1.5K+
GET150
GET1.5K
GET1.5K+
GEE150
GEE1.5K
GEE1.5K+
GEY100
GEY300
GR100
GR200
RXNS 100-1,000 150-1,500 150-1,500 150-1,500 100-300 100-200
Format
Reagent System
Reagent System
Reagent System
Reagent System
Reagent System
Reagent System
Sample
300 µl-10 ml blood
up to 5x107 cultured cells
up to 200 mg tissue
up to 1.5x109 bacterial cells
up to 2x108 yeast cells
up to 1 g plant tissue
Elution Volume
100 µl-1 ml
50-200 µl
100-200 µl
100 µl
50-100 µl
50-100 µl
Operation Time
<40 minutes
<60 minutes
<70 minutes
<60 minutes
<40 minutes
<50 minutes
Related Genomic DNA Extraction Products
Product Name RXNS Catalogue Number
150/1,500
GEE100/01K/01K+
100/1,000
GEB100/01K/01K+
150/1,500
GET150/1.5K/1.5K+
150/1,500
GEC150/1.5K/1.5K+
Geneaid™ DNA Isolation Kit (Yeast) 100, 300 GEY100/300
GENEzol™ DNA Reagent Plant
100, 200
GR100/200