Plant DNA Isolation Kit (GR100, GR200)

Genomic DNA Reagent Kit Plant
Plant DNA Isolation Kit (GR100, GR200)

GENEzol™ DNA Reagent Plant provides a quick and easy 3 step CTAB and chloroform based method to isolate total DNA (including genomic, mitochondrial and chloroplast DNA) from a variety of plant species (including algae and cyanobacteria). This unique reagent is able to lyse most common plant samples and plant samples with high a polysaccharide content. The extracted DNA is suitable for routine PCR screening, Real-Time PCR, Southern Blotting, Mapping and RFLP without phenol extraction.
 

 

 

Advantages (Cat. # GR100, GR200)
  • Extract high molecular weight genomic DNA from a variety of plant species
  • Sample Volume: up to 1 g of fresh plant tissue and up to 0.5 g of dry plant tissue
  • A cost effective, scalable solution
  • Format: simple and gentle CTAB and chloroform based DNA precipitation method
  • Storage: dry at room temperature (15-25ºC) for up to 2 years, RNase should be stored at 4ºC for extended periods
Applications
 
PCR, Real-Time PCR, Southern Blotting, Mapping, RFLP
 
Components
  • GENEzol™ DNA Reagent Plant
  • RNase A (50 mg/ml)
Quality Control
 
GENEzol™ DNA Reagent Plant is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. 50 mg of fresh Arabidopsis leaves are initially ground in GENEzol™ DNA Reagent Plant. A 15 µl aliquot of extracted genomic DNA from a 100 µl eluate is analyzed by electrophoresis on a 1% agarose gel.
 
GENEzol™  DNA Reagent Plant Functional Test Data

Figure 1. Genomic DNA (approximately 30 kb) was extracted using GENEzol™ DNA Reagent Plant. 50 mg of fresh Arabidopsis leaves were initially ground in GENEzol™ DNA Reagent Plant. A 15 µl aliquot of extracted genomic DNA from a 50 µl eluate was analyzed by electrophoresis on a 1% agarose gel. M = Geneaid 1 Kb DNA Ladder
Test DNA Conc. 260/280 260/230 Yield
1 380.7 µg/ml 1.97 1.88 19.04 µg
2 316.9 µg/ml 1.96 1.89 15.85 µg

Figure 1. Genomic DNA (approximately 30 kb) was extracted using GENEzol™ DNA Reagent Plant. 50 mg of fresh rose leaves were initially ground in GENEzol™ DNA Reagent Plant. A 5 µl aliquot of extracted genomic DNA from a 100 µl eluate was analyzed by electrophoresis on a 1% agarose gel. M = Geneaid 1 Kb DNA Ladder

Test DNA Conc. 260/280 260/230 Yield
1 109.5 µg/ml 1.82 1.71 11.0 µg
2 106.0 µg/ml 1.82 1.73 10.6 µg
3 99.4 µg/ml 1.81 1.71 9.9 µg
Reagent Genomic DNA Extraction Kits Specifications
Specifications
Cat. #
GEB100
GEB01K
GEB01K+
GEC150
GEC1.5K
GEC1.5K+
GET150
GET1.5K
GET1.5K+
GEE150
GEE1.5K
GEE1.5K+
GBY100
GBY300
GR100
GR200
RXNS 100-1,000 150-1,500 150-1,500 150-1,500 100-300 100-200
Format
Reagent System
Reagent System
Reagent System
Reagent System
Reagent System
Reagent System
Sample
300 µl-10 ml blood
up to 5x107 cultured cells
up to 200 mg tissue
up to 1.5x109 bacterial cells
up to 2x108 yeast cells
up to 1 g plant tissue
Elution Volume
100 µl-1 ml
50-200 µl
100-200 µl
100 µl
50-100 µl
50-100 µl
Operation Time
<40 minutes
<60 minutes
<70 minutes
<60 minutes
<40 minutes
<50 minutes
Related Genomic DNA Extraction Products
Product Name RXNS Catalogue Number
150/1,500
GEE100/01K/01K+
100/1,000
GEB100/01K/01K+
150/1,500
GET150/1.5K/1.5K+
150/1,500
GEC150/1.5K/1.5K+
Geneaid™ DNA Isolation Kit (Yeast) 100, 300 GEY100/300
GENEzol™ DNA Reagent Plant 100, 200 GR100/200