Bacteria DNA Isolation Kit (GEE150, GEE1.5K)

Genomic DNA Isolation Kit Bacteria
Bacteria DNA Isolation Kit (GEE150, GEE1.5K)

The Geneaid™ DNA Isolation Kit (Bacteria) offers a simple and gentle reagent DNA precipitation method for isolating high molecular weight genomic and viral DNA from Gram (+) positive and Gram (-) negative bacteria cells, suitable for archiving or sensitive downstream applications. This highly versatile solution based system can be scaled proportionately in order to satisfy larger sample volumes providing a convenient sample-storage procedure with minimal hands on time. Initially cells are lysed in the presence of detergents and a proprietary DNA stabilization solution followed by RNase A treatment. Once proteins and other contaminants are removed DNA is precipitated then rehydrated. The high quality extracted DNA is ready for use in a variety of downstream applications.

Advantages (Cat. # GEE150, GEE1.5K, GEE1.5K+)

  • Isolate high molecular weight DNA for archiving or sensitive downstream applications
  • High yield, High quality DNA (A260/A280 = 1.8-2.0)
  • Sample: 0.5-1.5 x 109 bacteria cells
  • Easily scalable to satisfy larger bacteria sample volumes
  • Minimal hands on time
  • Convenient, simple and gentle reagent DNA precipitation method
  • Storage: dry at room temperature (15-25°C) for up to 2 years, Lysozyme is shipped at room temperature and should be stored at -20°C for up to 1 year

Applications

DNA archiving, PCR, AFLP, RFLP/PADP, Southern Blotting, Real-time PCR 

Components

  • Cell Lysis Buffer
  • Gram+ Buffer
  • Protein Removal Buffer 
  • DNA Hydration Buffer (10 mM Tris-HCL, 1 mM EDTA, pH8.0)
  • RNase A (10 mg/ml) - not included with GEE1.5K
  • Lysozyme - not included with GEE1.5K

Quality Control

The quality of the Geneaid™ DNA Isolation Kit (Bacteria) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Genomic DNA is isolated from 0.5-1.5 x 109 bacteria cells. The isolated DNA (5-15 µg with an A260/A280 ratio of 1.8–2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.

Reagent Genomic DNA Extraction Kits Specifications

Specifications
Cat. #
GEB100
GEB01K
GEB01K+
GEC150
GEC1.5K
GEC1.5K+
GET150
GET1.5K
GET1.5K+
GEE150
GEE1.5K
GEE1.5K+
GEY100
GEY300
GR100
GR200
RXNS 100-1,000 150-1,500 150-1,500 150-1,500 100-300 100-200
Format
Reagent System
Reagent System
Reagent System
Reagent System
Reagent System
Reagent System
Sample
300 µl-10 ml blood
up to 5x107 cultured cells
up to 200 mg tissue
up to 1.5x109 bacterial cells
up to 2x108 yeast cells
up to 1 g plant tissue
Elution Volume
100 µl-1 ml
50-200 µl
100-200 µl
100 µl
50-100 µl
50-100 µl
Operation Time
<40 minutes
<60 minutes
<70 minutes
<60 minutes
<40 minutes
<50 minutes

Related Reagent DNA Precipitation Products

Product Name RXNS Catalogue Number
150/1,500
GEE100/01K/01K+
100/1,000
GEB100/01K/01K+
150/1,500
GET150/1.5K/1.5K+
150/1,500
GEC150/1.5K/1.5K+
Geneaid™ DNA Isolation Kit (Yeast) 100, 300 GEY100/300
GENEzol™ DNA Reagent Plant 100, 200 GR100/200