Blood DNA Isolation Kit (GEB100, GEB01K)

Genomic DNA Extraction Kit Blood Reagent
Blood DNA Isolation Kit (GEB100, GEB01K)

The Geneaid™ DNA Isolation Kit (Blood) offers a simple and gentle reagent DNA precipitation method for isolating high molecular weight genomic, mitochondrial or viral DNA suitable for archiving or sensitive downstream applications. This highly versatile solution based system can be scaled proportionately in order to satisfy larger sample volumes providing a convenient sample-storage procedure with minimal hands on time. Initially cells are lysed in the presence of detergents and a proprietary DNA stabilization solution followed by RNase A treatment. Once proteins and other contaminants are removed DNA is precipitated then rehydrated. The high quality extracted DNA is ready for use in a variety of downstream applications.

Advantages (Cat. # GEB100, GEB01K, GEB01K+)

  • Isolate high molecular weight DNA for archiving or sensitive downstream applications
  • High yield, High quality DNA (A260/A280 = 1.8-2.0)
  • Sample: 300 µl of whole blood, 3 ml of whole blood, 10 ml of whole blood, 10 ml of compromised whole blood
  • Easily scalable to satisfy larger blood sample volumes
  • Minimal hands on time
  • Convenient, simple and gentle reagent DNA precipitation method
  • Storage: dry at room temperature (15-25ºC), RNase A should be stored at 4ºC for extended periods

Applications

DNA archiving, PCR, AFLP, RFLP/PADP, Southern Blotting, Real-time PCR 

Components

  • RBC Lysis Buffer
  • Cell Lysis Buffer
  • Protein Removal Buffer 
  • DNA Hydration Buffer (10 mM Tris-HCL, 1 mM EDTA, pH8.0)
  • RNase A (10 mg/ml) - not included with GEB01K

Quality Control

The quality of the Geneaid™ DNA Isolation Kit (Blood) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Genomic DNA is isolated from 300 µl of fresh whole human blood. The isolated DNA (5-15 µg with an A260/A280 ratio of 1.8–2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.

Geneaid™ DNA Isolation Kit (Blood) Functional Test Data

Figure 1. High molecular weight genomic DNA (100-200 kb) was extracted using both the Geneaid™ DNA Isolation Kit (Blood) and the Gentra® Puregene® Blood Kit. Genomic DNA was extracted from 300 µl of whole human blood and resolved in 100 µl of DNA Hydration Buffer. 5 µl of the resolved genomic DNA was analyzed by electrophoresis on a 1% agarose gel.

Lane 1-3 =  Geneaid™ DNA Isolation Kit (Blood)
Lane 4-6 = Gentra® Puregene® Blood Kit

Reagent Genomic DNA Extraction Kits Specifications

Specifications
Cat. #
GEB100
GEB01K
GEB01K+
GEC150
GEC1.5K
GEC1.5K+
GET150
GET1.5K
GET1.5K+
GEE150
GEE1.5K
GEE1.5K+
GEY100
GEY300
GR100
GR200
RXNS 100-1,000 150-1,500 150-1,500 150-1,500 100-300 100-200
Format
Reagent System
Reagent System
Reagent System
Reagent System
Reagent System
Reagent System
Sample
300 µl-10 ml blood
up to 5x107 cultured cells
up to 200 mg tissue
up to 1.5x109 bacterial cells
up to 2x108 yeast cells
up to 1 g plant tissue
Elution Volume
100 µl-1 ml
50-200 µl
100-200 µl
100 µl
50-100 µl
50-100 µl
Operation Time
<40 minutes
<60 minutes
<70 minutes
<60 minutes
<40 minutes
<50 minutes

Related Reagent DNA Precipitation Products

Product Name RXNS Catalogue Number
150/1,500
GEE100/01K/01K+
100/1,000
GEB100/01K/01K+
150/1,500
GET150/1.5K/1.5K+
150/1,500
GEC150/1.5K/1.5K+
Geneaid™ DNA Isolation Kit (Yeast) 100, 300 GEY100/300
GENEzol™ DNA Reagent Plant 100, 200 GR100/200