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Cell DNA Isolation Kit (GEC150, GEC01K)

Genomic DNA Isolation Kit Cultured Cell
Cell DNA Isolation Kit (GEC150, GEC01K)

The Geneaid™ DNA Isolation Kit (Cultured Cell) offers a simple and gentle reagent DNA precipitation method for isolating high molecular weight genomic, mitochondrial and viral DNA from cultured cells, suitable for archiving or sensitive downstream applications. This highly versatile solution based system can be scaled proportionately in order to satisfy larger sample volumes providing a convenient sample-storage procedure with minimal hands on time. Initially cells are lysed in the presence of detergents and a proprietary DNA stabilization solution followed by RNase A treatment. Once proteins and other contaminants are removed DNA is precipitated then rehydrated. The high quality extracted DNA is ready for use in a variety of downstream applications.

Advantages (Cat. # GEC150, GEC1.5K, GEC1.5K+)

  • Isolate high molecular weight DNA for archiving or sensitive downstream applications
  • High yield, High quality DNA (A260/A280 = 1.8-2.0)
  • Sample: 0.5-1.5 x 109 cultured cells
  • Easily scalable to satisfy larger cultured cell sample volumes
  • Minimal hands on time
  • Convenient, simple and gentle reagent DNA precipitation method
  • Storage: dry at room temperature (15-25ºC) for up to 2 years, RNase A should be stored at -20ºC for extended periods

Applications

DNA archiving, PCR, AFLP, RFLP/PADP, Southern Blotting, Real-time PCR 

Components

  • Cell Lysis Buffer
  • Protein Removal Buffer 
  • DNA Hydration Buffer (10 mM Tris-HCL, 1 mM EDTA, pH8.0)
  • RNase A (10 mg/ml) - not included with GEC1.5K

Quality Control

The quality of the Geneaid™ DNA Isolation Kit (Cultured Cell) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Genomic DNA is isolated from 0.5-1.5 x 109 cultured cells. The isolated DNA (5-15 µg with an A260/A280 ratio of 1.8–2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.

Reagent Genomic DNA Extraction Kits Specifications

Specifications
Cat. #
GEB100
GEB01K
GEB01K+
GEC150
GEC1.5K
GEC1.5K+
GET150
GET1.5K
GET1.5K+
GEE150
GEE1.5K
GEE1.5K+
GEY100
GEY300
GR100
GR200
RXNS 100-1,000 150-1,500 150-1,500 150-1,500 100-300 100-200
Format
Reagent System
Reagent System
Reagent System
Reagent System
Reagent System
Reagent System
Sample
300 µl-10 ml blood
up to 5x107 cultured cells
up to 200 mg tissue
up to 1.5x109 bacterial cells
up to 2x108 yeast cells
up to 1 g plant tissue
Elution Volume
100 µl-1 ml
50-200 µl
100-200 µl
100 µl
50-100 µl
50-100 µl
Operation Time
<40 minutes
<60 minutes
<70 minutes
<60 minutes
<40 minutes
<50 minutes

Related Reagent DNA Precipitation Products

Product Name RXNS Catalogue Number
150/1,500
GEE100/01K/01K+
100/1,000
GEB100/01K/01K+
150/1,500
GET150/1.5K/1.5K+
150/1,500
GEC150/1.5K/1.5K+
Geneaid™ DNA Isolation Kit (Yeast) 100, 300 GEY100/300
GENEzol™ DNA Reagent Plant 100, 200 GR100/200
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