Cell DNA Isolation Kit (GEC150, GEC01K)

The Geneaid™ DNA Isolation Kit (Cultured Cell) offers a simple and gentle reagent DNA precipitation method for isolating high molecular weight genomic, mitochondrial and viral DNA from cultured cells, suitable for archiving or sensitive downstream applications. This highly versatile solution based system can be scaled proportionately in order to satisfy larger sample volumes providing a convenient sample-storage procedure with minimal hands on time. Initially cells are lysed in the presence of detergents and a proprietary DNA stabilization solution followed by RNase A treatment. Once proteins and other contaminants are removed DNA is precipitated then rehydrated. The high quality extracted DNA is ready for use in a variety of downstream applications.

Protocol download

Advantages (Cat. # GEC150, GEC1.5K, GEC1.5K+)

Isolate high molecular weight DNA for archiving or sensitive downstream applications
High yield, High quality DNA (A260/A280 = 1.8-2.0)
Sample: 0.5-1.5 x 10⁹ cultured cells
Easily scalable to satisfy larger cultured cell sample volumes
Minimal hands on time
Convenient, simple and gentle reagent DNA precipitation method
Storage: dry at room temperature (15-25ºC) for up to 2 years, RNase A should be stored at -20ºC for extended periods

Applications

DNA archiving, PCR, AFLP, RFLP/PADP, Southern Blotting, Real-time PCR

Components

Cell Lysis Buffer
Protein Removal Buffer
DNA Hydration Buffer (10 mM Tris-HCL, 1 mM EDTA, pH8.0)
RNase A (10 mg/ml) - not included with GEC1.5K

Quality Control

The quality of the Geneaid™ DNA Isolation Kit (Cultured Cell) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Genomic DNA is isolated from 0.5-1.5 x 10⁹ cultured cells. The isolated DNA (5-15 µg with an A260/A280 ratio of 1.8–2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.

Reagent Genomic DNA Extraction Kits Specifications

Specifications	Geneaid™ DNA Isolation Kit Blood	Geneaid™ DNA Isolation Kit Cultured Cell	Geneaid™ DNA Isolation Kit Tissue	Geneaid™ DNA Isolation Kit Bacteria	Geneaid™ DNA Isolation Kit Yeast	GENEzol™ DNA Reagent Plant
Cat. #	GEB100 GEB01K GEB01K+	GEC150 GEC1.5K GEC1.5K+	GET150 GET1.5K GET1.5K+	GEE150 GEE1.5K GEE1.5K+	GEY100 GEY300	GR100 GR200
RXNS	100-1,000	150-1,500	150-1,500	150-1,500	100-300	100-200
Format	Reagent System	Reagent System	Reagent System	Reagent System	Reagent System	Reagent System
Sample	300 µl-10 ml blood	up to 5x10⁷cultured cells	up to 200 mg tissue	up to 1.5x10⁹ bacterial cells	up to 2x10⁸ yeast cells	up to 1 g plant tissue
Elution Volume	100 µl-1 ml	50-200 µl	100-200 µl	100 µl	50-100 µl	50-100 µl
Operation Time	<40 minutes	<60 minutes	<70 minutes	<60 minutes	<40 minutes	<50 minutes

Related Reagent DNA Precipitation Products

Product Name	RXNS	Catalogue Number
Geneaid™ DNA Isolation Kit (Bacteria)	150/1,500	GEE100/01K/01K+
Geneaid™ DNA Isolation Kit (Blood)	100/1,000	GEB100/01K/01K+
Geneaid™ DNA Isolation Kit (Tissue)	150/1,500	GET150/1.5K/1.5K+
Geneaid™ DNA Isolation Kit (Cultured Cell)	150/1,500	GEC150/1.5K/1.5K+
Geneaid™ DNA Isolation Kit (Yeast)	100, 300	GEY100/300
GENEzol™ DNA Reagent Plant	100, 200	GR100/200