Cell DNA Isolation Kit (GEC150, GEC01K)
The Geneaid™ DNA Isolation Kit (Cultured Cell) offers a simple and gentle reagent DNA precipitation method for isolating high molecular weight genomic, mitochondrial and viral DNA from cultured cells, suitable for archiving or sensitive downstream applications. This highly versatile solution based system can be scaled proportionately in order to satisfy larger sample volumes providing a convenient sample-storage procedure with minimal hands on time. Initially cells are lysed in the presence of detergents and a proprietary DNA stabilization solution followed by RNase A treatment. Once proteins and other contaminants are removed DNA is precipitated then rehydrated. The high quality extracted DNA is ready for use in a variety of downstream applications.
Advantages (Cat. # GEC150, GEC1.5K, GEC1.5K+)
- Isolate high molecular weight DNA for archiving or sensitive downstream applications
- High yield, High quality DNA (A260/A280 = 1.8-2.0)
- Sample: 0.5-1.5 x 109 cultured cells
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Easily scalable to satisfy larger cultured cell sample volumes
- Minimal hands on time
- Convenient, simple and gentle reagent DNA precipitation method
- Storage: dry at room temperature (15-25ºC) for up to 2 years, RNase A should be stored at -20ºC for extended periods
Applications
DNA archiving, PCR, AFLP, RFLP/PADP, Southern Blotting, Real-time PCR
Components
- Cell Lysis Buffer
- Protein Removal Buffer
- DNA Hydration Buffer (10 mM Tris-HCL, 1 mM EDTA, pH8.0)
- RNase A (10 mg/ml) - not included with GEC1.5K
Quality Control
The quality of the Geneaid™ DNA Isolation Kit (Cultured Cell) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Genomic DNA is isolated from 0.5-1.5 x 109 cultured cells. The isolated DNA (5-15 µg with an A260/A280 ratio of 1.8–2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.
Reagent Genomic DNA Extraction Kits Specifications
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Specifications
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Cat. #
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GEB100
GEB01K
GEB01K+
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GEC150
GEC1.5K
GEC1.5K+
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GET150
GET1.5K
GET1.5K+
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GEE150
GEE1.5K
GEE1.5K+
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GEY100
GEY300
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GR100
GR200
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| RXNS | 100-1,000 | 150-1,500 | 150-1,500 | 150-1,500 | 100-300 | 100-200 |
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Format
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Reagent System
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Reagent System
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Reagent System
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Reagent System
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Reagent System
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Reagent System
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Sample
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300 µl-10 ml blood
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up to 5x107 cultured cells
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up to 200 mg tissue
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up to 1.5x109 bacterial cells
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up to 2x108 yeast cells
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up to 1 g plant tissue
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Elution Volume
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100 µl-1 ml
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50-200 µl
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100-200 µl
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100 µl
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50-100 µl
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50-100 µl
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Operation Time
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<40 minutes
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<60 minutes
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<70 minutes
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<60 minutes
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<40 minutes
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<50 minutes
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Related Reagent DNA Precipitation Products
| Product Name | RXNS | Catalogue Number |
| 150/1,500 |
GEE100/01K/01K+
|
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| 100/1,000 |
GEB100/01K/01K+
|
|
| 150/1,500 |
GET150/1.5K/1.5K+
|
|
| 150/1,500 |
GEC150/1.5K/1.5K+
|
|
| Geneaid™ DNA Isolation Kit (Yeast) | 100, 300 | GEY100/300 |
| GENEzol™ DNA Reagent Plant | 100, 200 | GR100/200 |
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