Total RNA Purification

Advantages (Cat. # RBY050, RBY100, RBY300)

• Purify total RNA within 20 minutes!
• High Yield: 5-30 μg of pure RNA
• Convenient: Includes premixed Sorbitol Buffer
• Sample: up to 5 x 10⁷ yeast or other fungus species
• RNA glass fiber spin column (yellow membrane optimized for total RNA purification from yeast)
• Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
• Storage: dry at room temperature (15-25°C) for up to 9 months

Applications

RT-PCR, Northern Blotting, Primer Extension, mRNA Selection, cDNA Synthesis

Components

• Sorbitol Buffer 
• RB Buffer
• W1 Buffer
• Wash Buffer
• RNase-free Water
• RB Columns
• 2 ml Collection Tubes

Quality Control

The quality of the Presto™ Mini RNA Yeast Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. The purified RNA  (A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.

Presto™ Mini RNA Yeast Kit Functional Test Data

Figure 1. Total RNA was extracted using the Presto™ Mini RNA Yeast Kit. Saccharomyces cerevisiae (5×107) was harvested by centrifugation at 5,000 x g for 10 minutes. A 5 µl aliquot of purified RNA from a 50 µl eluate was analyzed by electrophoresis on a 0.8% agarose gel. M = Geneaid 1 Kb DNA Ladder Test RNA Conc. 260/280 260/230 Yield 1 391.0 µg/ml 2.19 2.48 19.6 µg 2 389.9 µg/ml 2.19 2.51 19.5 µg 3 387.9 µg/ml 2.20 2.51 19.4 µg

Related RNA Purification Products

Advantages (Cat. # RBB050, RBB100, RBB300)

• Purify total RNA within 20 minutes!
• High Yield: up to 60 μg of pure RNA (1 x 10⁹ Escherichia coli: 40-45 µg, 1 x 10⁹ Bacillus subtilis: 50-55 µg)
• Wide Variety of Bacteria Sample Strains: Gram (+) Positive and Gram (-) Negative bacterial cells (up to 1 x 10⁹)
• Convenient: Includes Bacteria Lysis Buffer and Lysozyme
• RNA glass fiber spin column (yellow membrane optimized for total RNA purification from bacteria)
• Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
• Storage: dry at room temperature (15-25°C) for up to 9 months, Lysozyme should be stored at -20°C for extended periods

• Bacteria Lysis Buffer
• Lysozyme
• RB Buffer
• W1 Buffer
• Wash Buffer
• RNase-free Water
• RB Columns
• 2 ml Collection Tubes

Quality Control

The quality of the Presto™ Mini RNA  Bacteria Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. The purified RNA from Escherichia coli cells (up to 1 x 10⁹), (A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.

Presto™ Mini RNA Bacteria Kit Functional Test Data



Figure 1. RNA was extracted using the Presto™ Mini RNA Bacteria Kit. An Escherichia coli (1×109) culture (OD600=1.3, 1 ml) was harvested by centrifugation at 16,000 x g for 1 minute. 10 µl from a 50 µl eluate of purified RNA was analyzed by electrophoresis on a 0.8% agarose gel. Sample Yield 260/280 260/230 1 41.56 µg 2.14 2.35 2 40.87 µg 2.15 2.32

Related RNA Purification Products

Advantages (Cat. # RMI100)

• Purify miRNA within 30 minutes!
• High yield of miRNA and other small RNA
• Efficient removal of larger RNA for sensitive downstream applications
• Sample: 100 mg of tissue, up to 1 x 10⁶ cultured cells
• miRNA glass fiber spin columns (yellow membrane optimized for miRNA extraction)
• Individually packed miRNA spin columns and collection tubes, certified RNase and DNase-free
• Micropestle: certified RNase and DNase-free
• Storage: dry at room temperature (15-25ºC) for up to 9 months

Applications

miRNA analysis, siRNA analysis, shRNA analysis and snRNA analysis, qPCR, microarray analysis

Components

• Lysis Buffer
• Mi Buffer
• Wash Buffer
• Release Buffer
• RNA Columns
• 2 ml Collection Tubes
• Micropestles

Quality Control

The quality of the miRNA Isolation Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. 1/5 volume aliquots are analyzed on a polyacrylamide gel. The majority of RNA visible on the gel are <100 nt in size, with the major bands corresponding to tRNAs. The 5S and 5.8S rRNA species may also be visible. These tRNA and small rRNA bands should be clear and distinct. miRNA (21-22 nt) are typically not visible on the gel image.

miRNA Isolation Kit Functional Test Data

	Figure 1. Total RNA from mouse tissue was isolated using a reagent system.10 µg of each RNA was subjected to miRNA isolation using the miRNA Isolation Kit. The purified miRNA was resolved in 50 µl of Release Buffer and a 1/10 volume aliquot (5 µl) was analyzed by electrophoresis on a 2% agarose gel.
	Figure 2. Small RNAs were isolated from 4 human placental samples. Each lane contains 1/10 of the purified miRNA.

Related RNA Purification Products

Advantages (Cat. # RBP04, RBP10)

• Purify 96 total RNA samples within 30 minutes!
• High Yield: up to 30 μg/well of pure RNA
• Sample: cultured animal cells (up to 5 × 10⁶)
• Format: 96-well plates using either vacuum or centrifuge
• Storage: room temperature for up to 2 years

Applications

RT-PCR, Real-time PCR, Northern Blotting, mRNA Selection, Microarrays, cDNA Synthesis

Components

• RB Buffer
• W1 Buffer
• Wash Buffer
• RNase-free Water
• RNA Binding Plates
• 0.35 ml Collection Plates
• Adhesive Film

Quality Control

The quality of the 96-Well Total RNA Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Total RNA from cultured animal cells (up to 5 × 10⁶) is extracted using the Total RNA Mini Kit (Blood/Cultured Cell). The purified RNA is quantified with a spectrophotometer and checked by electrophoresis.

Advantages (Cat. # RPM10, RPM25)

• Large Sample Size: up to 1 g (fresh plant tissue)
• High Yield: 50-300 μg of pure RNA
• RNA glass fiber maxi spin column (white membrane optimized for RNA purification from large samples)
• Storage: room temperature for up to 2 years
• Operation time: within 1 hour

Applications

RT-PCR, Northern Blotting, Real-time PCR

Components

• RB Buffer
• PRB Buffer
• W1 Buffer
• Wash Buffer
• RNase-free Water
• Filter Columns
• RB Maxi Columns

Quality Control

The quality of the Total RNA Maxi Kit (Plant) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. 500 mg of fresh young leaf samples are purified using the Total RNA Maxi Kit (Plant). The Purified RNA is quantified with a spectrophotometer and checked by electrophoresis.

Related RNA Purification Products

• Purify total RNA within 15 minutes!
• High Yield: 5-30 μg of pure RNA
• Sample Size: up to 100 mg (fresh plant tissue), up to 25 mg (dry plant tissue)
• RNA glass fiber spin columns (yellow membrane optimized for total RNA extraction)
• Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
• Storage: dry at room temperature (15-25ºC) for up to 9 months

Applications

RT-PCR, Northern Blotting, Real-time PCR

Components

• RB Buffer
• PRB Buffer
• W1 Buffer
• Wash Buffer
• RNase-free Water
• Filter Columns
• RB Columns
• 2 ml Collection Tubes

Quality Control

The quality of the Total RNA Mini Kit (Plant) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Total RNA from a 50 mg fresh Thale Cress (Arabidopsis thaliana) leaves is extracted using the Total RNA Mini Kit (Plant). The purified RNA (27 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.

• Large Sample Size: up to 200 mg of tissue
• High Yield: 50-300 μg of pure RNA
• RNA glass fiber maxi spin column (white membrane optimized for RNA purification from large samples)
• Operation time: within 1 hour
• Storage: room temperature for up to 2 years

• RB Buffer
• W1 Buffer
• Wash Buffer
• RNase-free Water
• Filter Columns
• RB Maxi Columns

• Purify total RNA within 15 minutes!
• Sample: up to 25 mg of tissue, up to 25 mg of paraffin-embedded tissue
• High Yield: 5-30 μg of pure RNA
• RNase and DNase-free Micropestles included for simple tissue homogenization within the tube to reduce the risk of sample contamination
• Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
• RNA glass fiber spin columns (yellow membrane optimized for total RNA extraction)
• Storage: dry at room temperature (15-25ºC) for up to 9 months

• Micropestles
• RB Buffer
• W1 Buffer
• Wash Buffer
• RNase-free Water
• RB Columns
• Filter Columns
• 2 ml Collection Tubes

Quality Control

Advantages (Cat. # RBM10, RBM25)

• Large Sample Volume: up to 5 ml of whole blood, cultured cells (up to 1 x 10⁸), bacterial cells (up to 1 x 10¹⁰)
• High Yield: 50-300 μg of pure RNA
• RNA glass fiber maxi spin column (white membrane optimized for RNA purification from large samples)
• Operation time: within 1 hour

Components

• RBC Lysis Buffer
• RB Buffer
• RT Buffer
• W1 Buffer
• Wash Buffer
• RNase-free Water
• RB Maxi Columns

Quality Control

The quality of the Total RNA Maxi Kit (Blood/Cultured Cell) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. RNA from fresh whole blood and cultured cell samples are purified using the Total RNA Maxi Kit (Blood/Cultured Cell). The Purified RNA is quantified with a spectrophotometer and checked by electrophoresis.

• Purify total RNA within 20 minutes!
• High Yield: up to 2 µg from whole blood, up to 30 µg from 1x10⁶ 293T cells
• Sample Volume: up to 300 µl of whole blood, cultured cells (up to 5 x 10⁶)
• RNA glass fiber spin columns (yellow membrane optimized for total RNA extraction)
• Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
• Storage: dry at room temperature (15-25ºC) for up to 9 months

• RBC Lysis Buffer
• RB Buffer
• W1 Buffer
• Wash Buffer
• RNase-free Water
• RB Columns
• 2 ml Collection Tubes

Quality Control