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Blood/Cell DNA Maxi Kit (GDM010, GDM025)

Blood/Cell DNA Maxi Kit (GDM010, GDM025)
The Genomic DNA Maxi Kit (Blood/Cultured Cell) is optimized for DNA purification (including genomic, mitochondrial and viral DNA) from up to 10 ml of frozen blood or fresh blood and up to 1 x 108 cultured cells using an efficient maxiprep system. This DNA Extraction Kit uses Protease to reduce hemoglobin contamination while chaotropic salt and detergents are used to lyse cells and degrade protein, allowing the DNA to bind to the glass fiber matrix of the genomic DNA spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed in 1 hour without phenol/chloroform extraction or alcohol precipitation. Purified DNA, with approximately 20-30 kb, is suitable for use in PCR or other enzymatic reactions.

 

 

 

Advantages (Cat. # GDM010, GDM025)
  • High Yield: up to 200 μg from 10 ml of whole blood
  • Large Sample Size: up to 10 ml of frozen blood or fresh blood, cultured cells (up to 1 x 108)
  • Genomic glass fiber maxi spin column optimized for genomic DNA purification
  • Elution Volume: 500 µl ~ 2 ml
  • Storage: dry at room temperature (15-25°C)
  • Operation time: within 1 hour
Applications
 
PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR
 
Components
  • GB Buffer
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  • Protease
  • GD Maxi Columns
  • Collection Tubes
Quality Control
 
The quality of the Genomic DNA Maxi Kit (Blood/Cultured Cell) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Genomic DNA from a 10 ml of whole human blood is extracted using the Genomic DNA Maxi Kit (Blood/Cultured Cell). The purified DNA is quantified with a spectrophotometer and analyzed by electrophoresis.
 
Genomic DNA Maxi Kit Functional Test Data
Figure 1. Genomic DNA from 10 ml whole blood samples was extracted using the Genomic DNA Maxi Kit (Blood/Cultured Cell). 0.5 μl aliquots from 1 ml eluates of purified genomic DNA were analyzed by electrophoresis on a 0.8% agarose gel. M = Geneaid 1 Kb DNA Ladder
Sample DNA Conc. 260/280 260/230 Yield
1
180.6 ng/µl
1.84
2.28
180.6 µg
2
195.8 ng/µl
1.86
2.15
195.8 µg
3
187.5 ng/µl
1.84
2.21
187.5 µg

 
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