Magnetic Beads Micro Genomic DNA Extraction Kit MM048/MM096

Magnetic Beads Micro Genomic DNA Extraction Kit MM048/MM096

The Magnetic Beads Micro gDNA Extraction Kit was designed specifically for efficient genomic DNA purification from Whatman® FTA® Cards (dried blood spots). DNA is bound to the surface of the magnetic beads and released using a proprietary buffer system. The Magnetic Beads Micro gDNA Extraction Kit can be easily adapted to automated magnetic bead separation instruments and workstations. The purified DNA can be used in qPCR and a variety of other downstream applications.

Advantages (Cat. # MM048, MM096)
  • High Yield: 300 ng of Genomic DNA
  • High Quality DNA: A260/A280 = 1.8-2.0
  • Magnetic Bead Concentration: 50 mg/ml
  • Magnetic Bead Size: ~ 5 µm
  • Easily adapted to automated magnetic bead separation instruments and workstations
  • Sample: Whatman® FTA® Cards (dried blood spots)
  • Manual or automated DNA isolation
  • Operation time: within 50 minutes (manual) 
  • Storage: dry at room temperature (15-25ºC)
Applications
 
qPCR, PCR, Real-time PCR, Automated Fluorescent DNA Sequencing, Southern Blotting, Next Generation Sequencing (NGS)
 
Components
  • MM1 Buffer
  • MM2 Buffer
  • MM3 Buffer
  • Proteinase K
  • Carrier RNA
  • MW1 Buffer
  • MW2 Buffer
  • MM Magnetic Beads
  • Elution Buffer
Quality Control
 
The quality of the The Magnetic Beads Micro gDNA Extraction Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system by isolating genomic DNA from a 6 mm diamter circle cut out from Whatman® FTA® Cards (dried blood spots).
 
Magnetic Beads Micro gDNA Extraction Kit Functional Test Data 

Sample (Blood)
Volume
260/280
Yield
1 (Whatman® FTA® Card)
6.6 ng/µl
1.81
330 ng
2 (Whatman® FTA® Card)
5.5 ng/µl
1.89
275 ng
3 (Whatman® FTA® Card)
6.8 ng/µl
1.91
340 ng

Table 1. 300 ng of pure genomic DNA can be purified from a 6 mm diameter circle cut out from a Whatman® FTA® Card (dried blood spot).

Figure 1. Whole blood genomic DNA was extracted from 6 mm diameter circles cut out from Whatman® FTA® Cards (3 replications) using the Magnetic Beads Micro gDNA Extraction Kit. The purified DNA was eluted in 50 μl of Elution Buffer. A Real-time PCR assay was then performed with a 4 μl aliquot of purified DNA as template using the StepOnePlus™ Real-Time PCR system (Applied Biosystems).
Related Magnetic Beads Purification Products
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Magnetic Beads Genomic DNA Extraction Kit (Blood) 48, 96 MB048/96
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Magnetic Beads Micro gDNA Extraction Kit 48, 96 MM048/96
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