miRNA Isolation Kit (RMI050)

miRNA Isolation Kit
miRNA Isolation Kit (RMI050)
The miRNA Isolation Kit provides a quick and easy spin column system for purifying and enriching micro RNAs (miRNAs) and other small cellular RNAs from a wide variety of tissue and cells. Since miRNAs are vital for regulating gene expression, this kit is optimized for isolation of small RNA molecules while removing larger RNAs and minimizing genomic DNA contamination for improved sensitive downstream applications.

 

 

 

Advantages (Cat. # RMI050)

  • Purify miRNA within 30 minutes!
  • High yield of miRNA and other small RNA
  • Efficient removal of larger RNA for sensitive downstream applications
  • Sample: 100 mg of tissue, up to 1 x 106 cultured cells
  • Spin Columns: glass fiber membrane optimized for miRNA extraction
  • Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
  • Micropestle: certified RNase and DNase-free
  • Storage: dry at room temperature (15-25ºC) for up to 9 months

Applications

miRNA analysis, siRNA analysis, shRNA analysis and snRNA analysis, qPCR, microarray analysis

Components

  • Lysis Buffer
  • Mi Buffer
  • Wash Buffer
  • Release Buffer
  • RNA Columns
  • 2 ml Collection Tubes
  • Micropestles

Quality Control

The quality of the miRNA Isolation Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. 1/5 volume aliquots are analyzed on a polyacrylamide gel. The majority of RNA visible on the gel are <100 nt in size, with the major bands corresponding to tRNAs. The 5S and 5.8S rRNA species may also be visible. These tRNA and small rRNA bands should be clear and distinct. miRNA (21-22 nt) are typically not visible on the gel image.

miRNA Isolation Kit Functional Test Data

Figure 1. Total RNA from mouse tissue was isolated using a reagent system.10 µg of each RNA was subjected to miRNA isolation using the miRNA Isolation Kit. The purified miRNA was resolved in 50 µl of Release Buffer and a 1/10 volume aliquot (5 µl) was analyzed by electrophoresis on a 2% agarose gel.
Figure 2. Small RNAs were isolated from 4 human placental samples. Each lane contains 1/10 of the purified miRNA.

Total RNA Extraction Kits Specifications

Specifications
Cat. #
RB050
RB100
RB300
RT050
RT100
RT300
RP050
RP100
RP300
RBB050
RBB100
RBB300
RBY050
RBY100
RBY300
RMI050
GZX050
GZX100
GZX200
Format
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Tri Reagent Spin Column
Binding Capacity 50 µg 50 µg 50 µg 50 µg 50 µg 50 µg 50 µg
Sample
blood, cells
animal tissue plant tissue bacterial cells  yeast cells animal tissue, cells
blood,cells,
tissue, body fluids, bacteria
Micropestle N/A Yes N/A N/A N/A Yes N/A
Filter Column N/A Yes Yes N/A N/A N/A N/A
Enzyme
N/A
N/A
N/A
Lysozyme
N/A
N/A
N/A
Typical Yield
2-3 µg from
300 µl blood,
25-30 µg from
106 cells
5-30 µg
5-30 µg
40-50 µg from 1x109 bacterial cells
15-20 µg from 5x107
yeast cells
5-30 µg
5-30 µg 
Elution Volume 50 µl 50 µl 50 µl 50 µl 50 µl 50 µl 25-50 µl
Time <20 min. <15 min. <20 min. <20 min. <20 min. <30 min. <15 min.
Related RNA Extraction Products
Product Name                       RXNS              Catalogue Number
50, 100, 300
RB050/100/300, RBD050/100/300
50, 100, 300
RT050/100/300, RTD050/100/300
50, 100, 300
RP050/100/300, RPD050/100/300
50, 100, 300
RBB050/100/300, RBBD050/100/300
50, 100, 300
RBY050/100/300, RBYD050/100/300
50
RMI050
GENEzol™ TriRNA Pure Kit 50, 100, 200 GZX050/100/200, GZXD050/100/200
GENEzol™ 96 Well TriRNA Pure Kit 4 x 96, 10 x 96 96GZX04/10