RNA Extraction > Total RNA Purification

Total RNA Mini Kit (Plant)

RNA Kit Plant
Total RNA Mini Kit (Plant)

The Total RNA Mini Kit (Plant) is a RNA Isolation Kit designed for total RNA purification from plant tissue and cells using an efficient RNA miniprep system. This RNA Kit will purify RNA from a wide variety of plant species within 15 minutes. Samples are initially ground in liquid nitrogen and then filtered to remove cell debris using the efficient RNA filter columns. In the presence of a binding buffer and chaotropic salt, total RNA in the lysate binds to the glass fiber matrix of the RNA spin column. Once any contaminants have been removed using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water. Phenol extraction or alcohol precipitation is not required and the purified total RNA is ready for use in RT-PCR, Real-time PCR and Northern Blotting. Each Geneaid RNA Kit includes individually packaged certified DNase and RNase-free spin columns and collection tubes.

Protocol download

 

 

 

Advantages (Cat. # RP050, RP100, RP300)
  • Purify total RNA within 15 minutes!
  • High Yield: 5-30 μg of pure RNA
  • Sample Size: up to 100 mg (fresh plant tissue), up to 25 mg (dry plant tissue)
  • Spin Columns: glass fiber membrane optimized for total RNA extraction
  • Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
  • Storage: dry at room temperature (15-25ºC) for up to 9 months

Applications

RT-PCR, Northern Blotting, Real-time PCR

Components

  • RB Buffer
  • PRB Buffer
  • W1 Buffer
  • Wash Buffer
  • RNase-free Water
  • Filter Columns
  • RB Columns
  • 2 ml Collection Tubes

Quality Control

The quality of the Total RNA Mini Kit (Plant) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Total RNA from a 50 mg fresh Thale Cress (Arabidopsis thaliana) leaves is extracted using the Total RNA Mini Kit (Plant). The purified RNA (27 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.

Total RNA Mini Kit (Plant) Functional Test Data

Figure 1. Total RNA from 50 mg Thale Cress (Arabidopsis thaliana) leaves was extracted using the Total RNA Mini Kit (Plant). The purified RNA was analyzed by electrophoresis on a 1% agarose gel. M = Geneaid 1 Kb DNA Ladder

Test Yield (μg) 260/280 260/230
Test 1 27.02 2.07 2.43
Test 2 27.68 2.10 2.40
Test 3 26.15 2.06 2.44

Total RNA Extraction Kits Specifications

Specifications
Cat. #
RB050
RB100
RB300
RT050
RT100
RT300
RP050
RP100
RP300
RBB050
RBB100
RBB300
RBY050
RBY100
RBY300
RMI050
GZX050
GZX100
GZX200
Format
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
 Tri Reagent Spin Column
Binding Capacity 50 µg 50 µg 50 µg 50 µg 50 µg 50 µg 50 µg
Sample 300 µl blood 25 mg animal tissue 100 mg plant tissue 1x109 bacterial cells 5x107 yeast cells 100 mg animal tissue
blood
cultured cells
animal tissue
5x106 cultured cells         1x106 cultured cells
body fluids
plant tissue
bacteria
Micropestle N/A Yes N/A N/A N/A Yes N/A
Filter Column N/A Yes Yes N/A N/A N/A N/A
Enzyme
N/A
N/A
N/A
Lysozyme
Lyticase or
Zymolase
N/A
N/A
Typical Yield
2-3 µg from
300 µl blood
5-30 µg
5-30 µg
40-50 µg from 1x109 bacterial cells
15-20 µg from 5x107
yeast cells
5-30 µg
5-30 µg
25-30 µg from
106 cells
 
 
 
 
    
Elution Volume 50 µl 50 µl 50 µl 50 µl 50 µl 50 µl 25-50 µl
Operation Time <20 minutes <15 minutes <20 minutes <20 minutes <20 minutes <30 minutes <15 minutes

Related RNA Extraction Products

Product Name RXNS Catalogue Number
50, 100, 300
RB050/100/300
50, 100, 300
RT050/100/300
50, 100, 300
RP050/100/300
50, 100, 300
RBB050/100/300
50, 100, 300
RBY050/100/300
4 x 96, 10 x 96
RBP04/10
50
RMI050
GENEzol™ TriRNA Pure Kit 50, 100, 200 GZX050/100/200

 

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