Applications
RT-PCR, Northern Blotting, Real-time PCR
Components
Quality Control
The quality of the Total RNA Mini Kit (Plant) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Total RNA from a 50 mg fresh Thale Cress (Arabidopsis thaliana) leaves is extracted using the Total RNA Mini Kit (Plant). The purified RNA (27 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.
Total RNA Mini Kit (Plant) Functional Test Data
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Figure 1. Total RNA from 50 mg Thale Cress (Arabidopsis thaliana) leaves was extracted using the Total RNA Mini Kit (Plant). The purified RNA was analyzed by electrophoresis on a 1% agarose gel. M = Geneaid 1 Kb DNA Ladder
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Total RNA Extraction Kits Specifications
Specifications
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Cat. # |
RB050
RB100
RB300
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RT050
RT100
RT300
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RP050
RP100
RP300
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RBB050
RBB100
RBB300
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RBY050
RBY100
RBY300
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RMI050
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GZX050
GZX100
GZX200
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Format
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Spin Column
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Spin Column
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Spin Column
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Spin Column
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Spin Column
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Spin Column
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Tri Reagent Spin Column |
Binding Capacity | 50 µg | 50 µg | 50 µg | 50 µg | 50 µg | 50 µg | 50 µg |
Sample |
blood, cells
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animal tissue | plant tissue | bacterial cells | yeast cells | animal tissue, cells |
blood,cells,
tissue, body fluids, bacteria
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Micropestle | N/A | Yes | N/A | N/A | N/A | Yes | N/A |
Filter Column | N/A | Yes | Yes | N/A | N/A | N/A | N/A |
Enzyme |
N/A
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N/A
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N/A
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Lysozyme
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N/A
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N/A
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N/A
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Typical Yield
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2-3 µg from
300 µl blood,
25-30 µg from
106 cells
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5-30 µg
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5-30 µg
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40-50 µg from 1x109 bacterial cells
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15-20 µg from 5x107
yeast cells
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5-30 µg
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5-30 µg
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Elution Volume | 50 µl | 50 µl | 50 µl | 50 µl | 50 µl | 50 µl | 25-50 µl |
Time | <20 min. | <15 min. | <20 min. | <20 min. | <20 min. | <30 min. | <15 min. |
Product Name | RXNS | Catalogue Number |
50, 100, 300 |
RB050/100/300, RBD050/100/300
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50, 100, 300 |
RT050/100/300, RTD050/100/300
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50, 100, 300 |
RP050/100/300, RPD050/100/300
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50, 100, 300 |
RBB050/100/300, RBBD050/100/300
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50, 100, 300 |
RBY050/100/300, RBYD050/100/300
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50 |
RMI050
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GENEzol™ TriRNA Pure Kit | 50, 100, 200 | GZX050/100/200, GZXD050/100/200 |
GENEzol™ 96 Well TriRNA Pure Kit | 4 x 96, 10 x 96 | 96GZX04/10 |