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Plant Total RNA Mini Kit (RP050, RP100, RP300)
The Total RNA Mini Kit (Plant) is a RNA Isolation Kit designed for total RNA purification from plant tissue and cells using an efficient RNA miniprep system. This RNA Kit will purify RNA from a wide variety of plant species within 15 minutes. Samples are initially ground in liquid nitrogen and then filtered to remove cell debris using the efficient RNA filter columns. In the presence of a binding buffer and chaotropic salt, total RNA in the lysate binds to the glass fiber matrix of the RNA spin column. Once any contaminants have been removed using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water. Phenol extraction or alcohol precipitation is not required and the purified total RNA is ready for use in RT-PCR, Real-time PCR and Northern Blotting. Each Geneaid RNA Kit includes individually packaged certified DNase and RNase-free spin columns and collection tubes.
Advantages (Cat. # RP050, RP100, RP300)
- Purify total RNA within 15 minutes!
- High Yield: 5-30 μg of pure RNA
- Sample Size: up to 100 mg (fresh plant tissue), up to 25 mg (dry plant tissue)
- Spin Columns: glass fiber membrane optimized for total RNA extraction
- Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
- Storage: dry at room temperature (15-25°C)
Applications
RT-PCR, Northern Blotting, Real-time PCR
Components
- RB Buffer
- PRB Buffer
- W1 Buffer
- Wash Buffer
- RNase-free Water
- Filter Columns
- RB Columns
- 2 ml Collection Tubes
Quality Control
The quality of the Total RNA Mini Kit (Plant) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Total RNA from a 50 mg fresh Thale Cress (Arabidopsis thaliana) leaves is extracted using the Total RNA Mini Kit (Plant). The purified RNA (27 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.
Total RNA Mini Kit (Plant) Functional Test Data
 | Figure 1. Total RNA from 50 mg Thale Cress (Arabidopsis thaliana) leaves was extracted using the Total RNA Mini Kit (Plant). The purified RNA was analyzed by electrophoresis on a 1% agarose gel. M = Geneaid 1 Kb DNA Ladder
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Total RNA Extraction Kits Specifications
Specifications | |||||||
| Cat. # | RB050 RB100 RB300 | RT050 RT100 RT300 | RP050 RP100 RP300 | RBB050 RBB100 RBB300 | RBY050 RBY100 RBY300 | RMI050 | GZX050 GZX100 GZX200 |
Format | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column | Tri Reagent Spin Column |
| Binding Capacity | 50 µg | 50 µg | 50 µg | 50 µg | 50 µg | 50 µg | 50 µg |
| Sample | blood, cells | animal tissue | plant tissue | bacterial cells | yeast cells | animal tissue, cells | blood,cells, tissue, body fluids, bacteria |
| Micropestle | N/A | Yes | N/A | N/A | N/A | Yes | N/A |
| Filter Column | N/A | Yes | Yes | N/A | N/A | N/A | N/A |
| Enzyme | N/A | N/A | N/A | Lysozyme | N/A | N/A | N/A |
Typical Yield | 2-3 µg from 300 µl blood, 25-30 µg from 106 cells | 5-30 µg | 5-30 µg | 40-50 µg from 1x109 bacterial cells | 15-20 µg from 5x107 yeast cells | 5-30 µg | 5-30 µg |
| Elution Volume | 50 µl | 50 µl | 50 µl | 50 µl | 50 µl | 50 µl | 25-50 µl |
| Time | <20 min. | <15 min. | <20 min. | <20 min. | <20 min. | <30 min. | <15 min. |
Related RNA Extraction Products
| Product Name | RXNS | Catalogue Number |
| 50, 100, 300 | RB050/100/300, RBD050/100/300 | |
| 50, 100, 300 | RT050/100/300, RTD050/100/300 | |
| 50, 100, 300 | RP050/100/300, RPD050/100/300 | |
| 50, 100, 300 | RBB050/100/300, RBBD050/100/300 | |
| 50, 100, 300 | RBY050/100/300, RBYD050/100/300 | |
| 50 | RMI050 | |
| GENEzol™ TriRNA Pure Kit | 50, 100, 200 | GZX050/100/200, GZXD050/100/200 |
| GENEzol™ 96 Well TriRNA Pure Kit | 4 x 96, 10 x 96 | 96GZX04/10 |