Tissue Total RNA Mini Kit (RT050, RT100, RT300)

RNA Mini Kit Tissue
Tissue Total RNA Mini Kit (RT050, RT100, RT300)
The Total RNA Mini Kit (Tissue) is a RNA Isolation Kit designed for total RNA purification from a variety of animal tissue using an efficient RNA miniprep system. This RNA Kit will purify RNA from tissue samples within 15 minutes. Tissue samples can be efficiently homogenized in a microcentrifuge tube using the provided DNase and RNase-free Micropestle. The Total RNA Mini Kit (Tissue) uses detergents and chaotropic salt to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound by the glass fiber matrix of the RNA spin column. Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water. Phenol extraction or alcohol precipitation is not required and the purified RNA is ready for use in a variety of downstream applications. Each Geneaid RNA Kit includes individually packaged certified DNase and RNase-free spin columns and collection tubes.

 

 

 

Advantages (Cat. # RT050, RT100, RT300)
  • Purify total RNA within 15 minutes!
  • Sample: up to 25 mg of tissue (tailsnips, liver, kidney, brain, spleen, earpunches etc.)
  • High Yield: 5-30 μg of pure RNA
  • RNase and DNase-free Micropestles included for simple tissue homogenization within the tube to reduce the risk of sample contamination
  • Spin Columns: glass fiber membrane optimized for total RNA extraction
  • Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
  • Storage: dry at room temperature (15-25°C)
Applications
 
RT-PCR, Northern Blotting, Primer Extension, mRNA Selection, cDNA Synthesis, RNase Protection Assay
 
Components
  • RB Buffer
  • W1 Buffer
  • Wash Buffer
  • RNase-free Water
  • RB Columns
  • Filter Columns
  • 2 ml Collection Tubes
  • Micropestles

Quality Control

The quality of the Total RNA Mini Kit (Tissue) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Total RNA from a 25 mg fresh tissue sample is extracted using the Total RNA Mini Kit (Tissue). The purified RNA (A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.
 
Total RNA Mini Kit (Tissue) Functional Test Data 
Figure 1. Total RNA was extracted from 10 mg of various mouse tissue using the Total RNA Mini Kit (Tissue). 5 μl aliquots of each sample was analyzed by electrophoresis on a 1% agarose gel.
 
1. Brain, 2. Lung, 3. Liver, 4. Spleen, 5. Kidney

Total RNA Extraction Kits Specifications

Specifications
Cat. #
RB050
RB100
RB300
RT050
RT100
RT300
RP050
RP100
RP300
RBB050
RBB100
RBB300
RBY050
RBY100
RBY300
RMI050
GZX050
GZX100
GZX200
Format
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Tri Reagent Spin Column
Binding Capacity 50 µg 50 µg 50 µg 50 µg 50 µg 50 µg 50 µg
Sample
blood, cells
animal tissue plant tissue bacterial cells  yeast cells animal tissue, cells
blood,cells,
tissue, body fluids, bacteria
Micropestle N/A Yes N/A N/A N/A Yes N/A
Filter Column N/A Yes Yes N/A N/A N/A N/A
Enzyme
N/A
N/A
N/A
Lysozyme
N/A
N/A
N/A
Typical Yield
2-3 µg from
300 µl blood,
25-30 µg from
106 cells
5-30 µg
5-30 µg
40-50 µg from 1x109 bacterial cells
15-20 µg from 5x107
yeast cells
5-30 µg
5-30 µg 
Elution Volume 50 µl 50 µl 50 µl 50 µl 50 µl 50 µl 25-50 µl
Time <20 min. <15 min. <20 min. <20 min. <20 min. <30 min. <15 min.
Related RNA Extraction Products
Product Name                       RXNS              Catalogue Number
50, 100, 300
RB050/100/300, RBD050/100/300
50, 100, 300
RT050/100/300, RTD050/100/300
50, 100, 300
RP050/100/300, RPD050/100/300
50, 100, 300
RBB050/100/300, RBBD050/100/300
50, 100, 300
RBY050/100/300, RBYD050/100/300
50
RMI050
GENEzol™ TriRNA Pure Kit 50, 100, 200 GZX050/100/200, GZXD050/100/200
GENEzol™ 96 Well TriRNA Pure Kit 4 x 96, 10 x 96 96GZX04/10