RNA Extraction > Total RNA Purification

Presto™ Mini RNA Yeast Kit

Presto™ Mini RNA Yeast Kit
Presto™ Mini RNA Yeast Kit

The Presto™ Mini RNA Yeast Kit was designed for total RNA purification from yeast and a wide variety of other fungi using an efficient RNA miniprep system. This RNA Kit includes Sorbitol Buffer to reduce sample preparation time and minimize hands on time.  The Presto™ Mini RNA Yeast Kit uses detergents and chaotropic salt to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound by the glass fiber matrix of the spin column and once any contaminants have been removed, using the Wash Buffer, the purified total RNA is eluted by RNase-free Water. High quality total RNA can be purified within 20 minutes and is ready for use in RT-PCR, Northern Blotting, Primer Extension, mRNA Selection and cDNA Synthesis. Each Geneaid RNA Kit includes individually packaged certified DNase and RNase-free spin columns and collection tubes.

Protocol download

 

 

 

Advantages (Cat. # RBY050, RBY100, RBY300)

  • Purify total RNA within 20 minutes!
  • High Yield: 5-30 μg of pure RNA
  • Convenient: Includes premixed Sorbitol Buffer
  • Sample: up to 5 x 107 yeast or other fungus species
  • RNA glass fiber spin column (yellow membrane optimized for total RNA purification from yeast)
  • Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
  • Storage: dry at room temperature (15-25°C) for up to 9 months

Applications

RT-PCR, Northern Blotting, Primer Extension, mRNA Selection, cDNA Synthesis

Components

  • Sorbitol Buffer 
  • RB Buffer
  • W1 Buffer
  • Wash Buffer
  • RNase-free Water
  • RB Columns
  • 2 ml Collection Tubes

Quality Control

The quality of the Presto™ Mini RNA Yeast Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. The purified RNA  (A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.

Presto™ Mini RNA Yeast Kit Functional Test Data

Figure 1. Total RNA was extracted using the Presto™ Mini RNA Yeast Kit. Saccharomyces cerevisiae (5×107) was harvested by centrifugation at 5,000 x g for 10 minutes. A 5 µl aliquot of purified RNA from a 50 µl eluate was analyzed by electrophoresis on a 0.8% agarose gel.
M = Geneaid 1 Kb DNA Ladder
Test RNA Conc. 260/280 260/230 Yield
1 391.0 µg/ml 2.19 2.48 19.6 µg
2 389.9 µg/ml 2.19 2.51 19.5 µg
3 387.9 µg/ml 2.20 2.51 19.4 µg

Related RNA Purification Products

Product Name                                                                  
RXNS           
Catalogue  Number
Presto™ Mini RNA Yeast Kit
 50, 100, 300
RBY050/100/300
50, 100, 300
RT050/100/300
50, 100, 300
RP050/100/300
50, 100, 300
RB050/100/300
10, 25
RTM10/25
10, 25
RPM10/25
10, 25
RBM10/25
50, 100, 300
RBB050/100/300
4 x 96, 10 x 96
RBP04/10
50
RMI050

 

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