Yeast Total RNA Mini Kit (RBY050, RBY100, RBY300)

Yeast RNA Kit
Yeast Total RNA Mini Kit (RBY050, RBY100, RBY300)
The Presto™ Mini RNA Yeast Kit was designed for total RNA purification from yeast and a wide variety of other fungus species using an efficient RNA miniprep system. This RNA Kit includes Sorbitol Buffer to reduce sample preparation time and minimize hands on time.  The Presto™ Mini RNA Yeast Kit uses detergents and chaotropic salt to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound by the glass fiber matrix of the spin column and once any contaminants have been removed, using the Wash Buffer, the purified total RNA is eluted by RNase-free Water. High quality total RNA can be purified within 20 minutes and is ready for use in RT-PCR, Northern Blotting, Primer Extension, mRNA Selection and cDNA Synthesis. Each Geneaid RNA Kit includes individually packaged certified DNase and RNase-free spin columns and collection tubes.

 

 

 

Advantages (Cat. # RBY050, RBY100, RBY300)

  • Purify total RNA within 20 minutes!
  • High Yield: 5-30 μg of pure RNA
  • Convenient: Includes premixed Sorbitol Buffer
  • Sample: up to 5 x 107 yeast or other fungus species
  • Spin Columns: glass fiber membrane optimized for total RNA extraction
  • Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
  • Storage: dry at room temperature (15-25°C)

Applications

RT-PCR, Northern Blotting, Primer Extension, mRNA Selection, cDNA Synthesis

Components

  • Sorbitol Buffer 
  • RB Buffer
  • W1 Buffer
  • Wash Buffer
  • RNase-free Water
  • RB Columns
  • 2 ml Collection Tubes

Quality Control

The quality of the Presto™ Mini RNA Yeast Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. The purified RNA  (A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.

Presto™ Mini RNA Yeast Kit Functional Test Data

Figure 1. Total RNA was extracted using the Presto™ Mini RNA Yeast Kit. Saccharomyces cerevisiae (5×107) was harvested by centrifugation at 5,000 x g for 10 minutes. A 5 µl aliquot of purified RNA from a 50 µl eluate was analyzed by electrophoresis on a 0.8% agarose gel.
M = Geneaid 1 Kb DNA Ladder
Test RNA Conc. 260/280 260/230 Yield
1 391.0 µg/ml 2.19 2.48 19.6 µg
2 389.9 µg/ml 2.19 2.51 19.5 µg
3 387.9 µg/ml 2.20 2.51 19.4 µg

Total RNA Extraction Kits Specifications

Specifications
Cat. #
RB050
RB100
RB300
RT050
RT100
RT300
RP050
RP100
RP300
RBB050
RBB100
RBB300
RBY050
RBY100
RBY300
RMI050
GZX050
GZX100
GZX200
Format
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Tri Reagent Spin Column
Binding Capacity 50 µg 50 µg 50 µg 50 µg 50 µg 50 µg 50 µg
Sample
blood, cells
animal tissue plant tissue bacterial cells  yeast cells animal tissue, cells
blood,cells,
tissue, body fluids, bacteria
Micropestle N/A Yes N/A N/A N/A Yes N/A
Filter Column N/A Yes Yes N/A N/A N/A N/A
Enzyme
N/A
N/A
N/A
Lysozyme
N/A
N/A
N/A
Typical Yield
2-3 µg from
300 µl blood,
25-30 µg from
106 cells
5-30 µg
5-30 µg
40-50 µg from 1x109 bacterial cells
15-20 µg from 5x107
yeast cells
5-30 µg
5-30 µg 
Elution Volume 50 µl 50 µl 50 µl 50 µl 50 µl 50 µl 25-50 µl
Time <20 min. <15 min. <20 min. <20 min. <20 min. <30 min. <15 min.
Related RNA Extraction Products
Product Name                       RXNS              Catalogue Number
50, 100, 300
RB050/100/300, RBD050/100/300
50, 100, 300
RT050/100/300, RTD050/100/300
50, 100, 300
RP050/100/300, RPD050/100/300
50, 100, 300
RBB050/100/300, RBBD050/100/300
50, 100, 300
RBY050/100/300, RBYD050/100/300
50
RMI050
GENEzol™ TriRNA Pure Kit 50, 100, 200 GZX050/100/200, GZXD050/100/200
GENEzol™ 96 Well TriRNA Pure Kit 4 x 96, 10 x 96 96GZX04/10