GENEzol™ Reagent (GZR050/GZR100/GZR200)

Cost Effective Trizol Alternative RNA Reagent
GENEzol™ Reagent (GZR050/GZR100/GZR200)

GENEzol™ Reagent is a phenol, chloroform and guanidine isothiocyanate based scalable solution for extracting high-quality total RNA as well as simultaneous extraction of RNA, DNA and protein from a wide variety of samples such as blood, buffy coat, plasma, serum, cultured cells and tissue. The extracted RNA can be used directly in a variety of downstream applications such as cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays and Northern Blotting. GENEzol™ Reagent is a cost effective alternative for efficient total RNA extraction.

 

 

Advantages (Cat. # GZR050, GZR100, GZR200)
  • Extract total RNA or simultaneous RNA, DNA and protein within 1 hour
  • Sample Volume: up to 300 μl of blood, buffy coat, serum, plasma, up to 5 x 106 cultured cells, 50-100 mg of tissue
  • A cost effective, scalable solution
  • Format: phenol, chlorform, guanidine isothiocyanate
  • Storage: dry at 2ºC to 25ºC
Applications
 
cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays, Northern Blotting
 
Components
  • GENEzol™ Reagent
Quality Control
 
GENEzol™ Reagent is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. RNA from a 1 ml human blood sample is extracted using GENEzol™ Reagent. 10 µl from a 50 µl eluate of RNA is analyzed by electrophoresis on a 0.8% agarose gel.
 
GENEzol™ Reagent Comparison Data
Figure 1. Bacterial RNA was extracted from 5 x 108 E. coli cells using our top competitor's TRI reagent and GENEzol™ Reagent. The E. coli cells were incubated with lysozyme for 5 minutes at room temperature. 1 ml of reagent was then added to the cell lysate. The RNA pellets were dissolved in 50 μl of RNase-free water.
TRI Reagent = 1, 2
GENEzol™ Reagent = 3, 4
Sample
ng/µl
260/280
260/230
Yield (µg)
1
241.3
1.77
0.66
12.1
2
260.2
1.83
0.85
13.0
3
315.6
1.78
0.75
15.8
4 293.4 1.81 0.80 14.7

GENEzol™ Reagent Functional Test Data


Figure 2. RNA from a 1 ml human blood sample was extracted using GENEzol™ Reagent. 10 µl from a 50 µl eluate of RNA was analyzed by electrophoresis on a 0.8% agarose gel. M = Geneaid 1 Kb DNA Ladder
Test RNA Conc. 260/280 Yield
1 119.9 µg/ml 1.82 6.0 µg
2 135.5 µg/ml 1.76 6.8 µg
3 176.1 µg/ml 1.77 8.8 µg
GENEzol™ Reagent real-time PCR Data
 
RNA was extracted from 300 µl of human blood (2.8 x 1010 copy/ml) using GENEzol™ Reagent.
 
RNA standard (107 copy/ml)
RNA standard (106 copy/ml)
RNA standard (105 copy/ml)
 
 
 
RNA standard (104 copy/ml)
 
 
 

Figure 3. Quantitative analysis of human beta globin mRNA extracted by GENEzol™ using Taqman probe 1-step qRT-PCR assay. The assay was run on a BioRad IQ5 thermal cycler.
 
Related RNA Extraction and Purification Products
Product Name RXNS Catalogue Number
50, 100, 200
GZR050/100/200
50, 100, 200
GZX050/100/200, GZXD050/100/200
50, 100
GZB050/100
50, 100, 200
TRP050/100/200, TRPD050/100/200
50, 100
PR050/100
GENEzol™ 96 Well TriRNA Pure Kit 4 x 96, 10 x 96 96GZX04/10