GENEzol™ Bacteria Kit (GZBD050/GZBD100/GZBD200) with DNase

Bacteria RNA Kit with DNase I Set
GENEzol™ Bacteria Kit (GZBD050/GZBD100/GZBD200) with DNase
The GENEzol™ TriRNA Bacteria Kit is a phenol and guanidine isothiocyanate plus spin column system for convenient purification of high-quality total RNA from bacteria samples. Bacterial cell walls are initially lysed using Lysozyme. The sample is then homogenized in GENEzol™ Reagent without chloroform phase separation or isopropanol RNA precipitation. Following sample homogenization, simply bind, wash and elute the high-quality, total RNA in RNase-free Water and use in a variety of sensitive downstream applications.
 

 

 

Advantages (Cat. # GZBD050, GZBD100, GZBD200)
  • Purify total RNA within 20 minutes without chloroform phase separation or isopropanol RNA precipitation
  • Sample Volume: bacteria cells up to 1 x 109
  • Format: phenol, chlorform, guanidine isothiocyanate plus spin column system
  • High quality RNA: A260/A280 >1.8, A260/A230 >1.8
  • Storage: GENEzol™ Reagent should be stored at 2ºC to 25ºC, DNase I and Lysozyme can be shipped at room temperature but should be stored at -20ºC for extended periods
Applications
 
cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays, Northern Blotting
 
Components
  • GENEzol™ Reagent
  • Pre-Wash Buffer
  • Lysozyme
  • Bacteria Lysis Buffer
  • DNase I
  • DNase Reaction Buffer
  • Wash Buffer
  • RNase-free Water
  • RB Columns
  • 2 ml Collection Tubes
Quality Control
 
The GENEzol™ TriRNA Bacteria Kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. 5 µl from a 50 µl eluate of purified RNA is analyzed by electrophoresis on a 1% agarose gel.
 
GENEzol™ TriRNA Bacteria Kit Functional Test Data

Figure 1. RNA was extracted using the GENEzol™ TriRNA Bacteria Kit. An Escherichia coli (1×109) culture (OD600=2, 1 ml) was harvested by centrifugation at 16,000 x g for 2 minutes, followed by RNA extraction. 5 µl from a 50 µl eluate of RNA was analyzed by electrophoresis on a 1% agarose gel.
M = Geneaid 1 Kb DNA Ladder
Test RNA Concentration 260/280 260/230 Yield
1 502.6 µg/ml 2.06 2.18 25.13 µg
2 518.3 µg/ml 2.07 2.21 25.92 µg
3 506.0 µg/ml 2.08 2.24 25.30 µg
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