The GENEzol™ TriRNA Pure Kit is a phenol and guanidine isothiocyanate plus spin column system for convenient purification of high-quality total RNA from a variety of samples. Initially, samples are homogenized in GENEzol™ Reagent without chloroform phase separation or isopropanol RNA precipitation. Following sample homogenization, simply bind, wash and elute the high-quality, total RNA in RNase-free Water and use in a variety of sensitive downstream applications.
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Figure 1. RNA was purified using the GENEzol™ TriRNA Pure Kit in parallel to the similar product from competitor Z. 5x105 HeLa cells were homogenized using GENEzol™ Reagent and competitor Z tri reagent. RNA was then purified using the corresponing kits spin column procedure. 10 µl from a 50 µl eluate of purified RNA was analyzed by electrophoresis on a 0.8% agarose gel.
Table 1. Total RNA purified using the GENEzol™ TriRNA Pure Kit and competitor Z.
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Table 2. RNA was purified using the GENEzol™ TriRNA Pure Kit. RNA from 200 µl of whole human blood (3 donors) was initally homogenized using GENEzol™ Reagent. RNA was then purified using a spin column procedure. 10 µl from each 35 µl eluate of purified RNA was analyzed by electrophoresis on a 0.8% agarose gel. |
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RNA was extracted from 200 µl of human blood (5.2 x 1010 copy/ml) using GENEzol™ TriRNA Pure Kit. | |
RNA standard (107 copy/ml)
RNA standard (106 copy/ml)
RNA standard (105 copy/ml)
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RNA standard (104 copy/ml)
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Figure 3. Quantitative analysis of human beta globin mRNA purified by GENEzol™ TriRNA Pure Kit using a Taqman probe 1-step qRT-PCR assay. The assay was run on a BioRad IQ5 thermal cycler.
Product Name | RXNS | Catalogue Number |
50, 100, 200 |
GZR050/100/200
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50, 100, 200 |
GZX050/100/200, GZXD050/100/200
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50, 100 |
GZB050/100
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50, 100, 200 |
TRP050/100/200, TRPD050/100/200
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50, 100 |
PR050/100
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GENEzol™ 96 Well TriRNA Pure Kit | 4 x 96, 10 x 96 | 96GZX04/10 |