GENEzol™ 96 Well RNA Kit (96GZX04/96GZX10)
The GENEzol™ 96 Well TriRNA Pure Kit is a phenol and guanidine isothiocyanate plus 96 well RNA binding plate system for high-throughput purification of high-quality total RNA from a variety of samples. Initially, samples are homogenized in GENEzol™ Reagent without chloroform phase separation or isopropanol RNA precipitation. Following sample homogenization, simply bind, wash and elute the high-quality, total RNA in RNase-free Water and use in a variety of sensitive downstream applications.
Advantages (Cat. # 96GZX04, 96GZX10)
- Purify 96 wells of total RNA within 1 hour
- A cost effective phenol, guanidine isothiocyanate solution plus RNA binding plate system
- No chlorophorm phase separation
- No isopropanol RNA precipitation
- No phenol carryover
- Sample Volume: up to 150 μl of blood, buffy coat, serum, plasma, up to 2 x 106 cultured cells, 10-20 mg of tissue, up to 1 x 109 bacterial cells, 10-20 mg of plant tissue
- High quality RNA: A260/A280 >1.8, A260/A230 >1.8
- Binding Capacity: 50 µg RNA per well
- Binding Plates: glass fiber membrane optimized for total RNA extraction (certified RNase and DNase-free)
- Elution Volume: 60-80 µl (dead volume: 20-25 µl)
- Storage: GENEzol™ Reagent should be stored at 2ºC to 25ºC, other components should be stored at room temperature (15-25ºC)
Applications
cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays, Northern Blotting
Components
- GENEzol™ Reagent
- Pre-Wash Buffer
- Wash Buffer
- RNase-free Water
- Presto™ RNA 96 Well Binding Plates
- Microtubes (Racked)
- Microtubes (8-strip)
- Caps for Microtubes (8-strip)
- 96 Deep Well Plates
- 0.35 ml Collection Plates
- Adhesive Film
Quality Control
The GENEzol™ 96 Well TriRNA Pure Kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system.
GENEzol™ 96 Well TriRNA Pure Kit Functional Test Data
![]() | Figure 1. RNA was purified using the GENEzol™ 96 Well TriRNA Pure Kit in parallel to the similar product from competitor Z. 5x105 HeLa cells were homogenized using GENEzol™ Reagent and competitor Z tri reagent. RNA was then purified using the corresponing kits spin column procedure. 10 µl from a 50 µl eluate of purified RNA was analyzed by electrophoresis on a 0.8% agarose gel.
Table 1. Total RNA purified using the GENEzol™ 96 Well TriRNA Pure Kit and competitor Z. | |||||||||||||||||||||||||
Table 2. RNA was purified using the GENEzol™ 96 Well TriRNA Pure Kit. RNA from 200 µl of whole human blood (3 donors) was initally homogenized using GENEzol™ Reagent. RNA was then purified using a spin column procedure. 10 µl from each 35 µl eluate of purified RNA was analyzed by electrophoresis on a 0.8% agarose gel. |
GENEzol™ TriRNA Pure Kit real-time PCR Data
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RNA was extracted from 200 µl of human blood (5.2 x 1010 copy/ml) using GENEzol™ 96 Well TriRNA Pure Kit. | |
RNA standard (107 copy/ml) RNA standard (106 copy/ml) RNA standard (105 copy/ml) | |
RNA standard (104 copy/ml) | |
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Related RNA Extraction and Purification Products
Product Name | RXNS | Catalogue Number |
50, 100, 200 | GZR050/100/200 | |
50, 100, 200 | GZX050/100/200, GZXD050/100/200 | |
50, 100 | GZB050/100 | |
50, 100, 200 | TRP050/100/200, TRPD050/100/200 | |
50, 100 | PR050/100 | |
GENEzol™ 96 Well TriRNA Pure Kit | 4 x 96, 10 x 96 | 96GZX04/10 |